PAPER 2026 FULL ANSWER GUIDE
◉ Carbon (food) source for bacteria
Answer: what does the glucose in the medium do
◉ Differentiates between Bacteria that ferment glucose in the
presence of high salt (ex. Enterococci)
Bacteria that don't
Answer: what does the salt in the medium do
◉ pH indicator
Answer: what does the Phenol red in the medium do
◉ glucose fermentation occurs
Answer: what does it mean if the reaction is yellow
◉ glucose fermentation does not occur
Answer: what does it mean if the reaction is red
◉ Enterococcus and Streptococcus pyogenes
,Answer: what bacteria do we use in a salt tolerance test
◉ Enterococcus
Answer: which bacteria ferments glucose in the presence of salt
◉ Streptococcus pyogenes
Answer: which bacteria does not ferment glucose in the presence of
salt
◉ Enterococcus
Answer: which bacteria will give us the reaction on the left
◉ S. pyogenes
Answer: which bacteria will give us the reaction on the right
◉ Transfer one heavy loop of microbe into broth
Loosen caps before incubating
Answer: what is the procedure for a salt tolerance test
◉ MacConkey agar
Hektoen agar
Indole broth
, Citrate agar
Answer: what procedures used enteric (gram negative) bacteria
◉ gram negative
rod-shaped
facultative anaerobes
Answer: what are characteristics of enteric bacteria
◉ glucose to acid
Answer: what do enteric bacteria ferment
◉ occuring in the intestines
Answer: what does enteric mean
◉ human intestines
Answer: where does E. coli commonly inhabit
◉ fecal contaminated water
Answer: where does Shigella commonly inhabit
◉ undercooked chicken/eggs
Answer: where does Salmonella commonly inhabit