BIOL 2460 Final Exam Study ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
Guide exam with correct answers ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
Degerming
microbial numbers are significantly reduced by gently ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
scrubbing living tissue (skin) w/ soap to avoid ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
transmission of pathogenic microbes ||\\||\\ ||\\||\\ ||\\||\\
Soap Characteristics ||\\||\\
- salts of long-chain fatty acids
||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
- includes both polar & nonpolar regions
||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
- interacts w/ nonpolar oils & grease to create emulsions
||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
Soaps _____ _____ kill or inhibit microbial growth and are
||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
NOT considered antiseptics or disinfectants
||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
DO NOT ||\\||\\
Purpose of Aseptic Technique ||\\||\\ ||\\||\\ ||\\||\\
used in clinical & research settings to prevent
||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
contamination of sterile surfaces ||\\||\\ ||\\||\\ ||\\||\\
Sterile Surfaces ||\\||\\
, - human tissue
||\\||\\ ||\\||\\
- fomites
||\\||\\
- medical devices
||\\||\\ ||\\||\\
Aseptic Technique ||\\||\\
involves a combination of protocols that collectively ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
maintain sterility (asepsis) to prevent contamination of a ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
patient or surface w/ infectious agents ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
Sterile Field ||\\||\\
medical procedures that are performed in, a designated ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
area that is kept free of all vegetative microbes,
||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
endospores, & viruses ||\\||\\ ||\\||\\
Bunsen Burner ||\\||\\
metal apparatus that creates a flame that can be used to
||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
sterilize pieces of equipment ||\\||\\ ||\\||\\ ||\\||\\
BSL-2
- PPE (gloves, coat, & eye protection)
||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
- Sinks & eye wash stations
||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
- restricted access to the lab for authorized persons only
||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
- autoclaves or alternative method(s) of decontamination
||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
BSL-1
Guide exam with correct answers ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
Degerming
microbial numbers are significantly reduced by gently ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
scrubbing living tissue (skin) w/ soap to avoid ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
transmission of pathogenic microbes ||\\||\\ ||\\||\\ ||\\||\\
Soap Characteristics ||\\||\\
- salts of long-chain fatty acids
||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
- includes both polar & nonpolar regions
||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
- interacts w/ nonpolar oils & grease to create emulsions
||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
Soaps _____ _____ kill or inhibit microbial growth and are
||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
NOT considered antiseptics or disinfectants
||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
DO NOT ||\\||\\
Purpose of Aseptic Technique ||\\||\\ ||\\||\\ ||\\||\\
used in clinical & research settings to prevent
||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
contamination of sterile surfaces ||\\||\\ ||\\||\\ ||\\||\\
Sterile Surfaces ||\\||\\
, - human tissue
||\\||\\ ||\\||\\
- fomites
||\\||\\
- medical devices
||\\||\\ ||\\||\\
Aseptic Technique ||\\||\\
involves a combination of protocols that collectively ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
maintain sterility (asepsis) to prevent contamination of a ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
patient or surface w/ infectious agents ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
Sterile Field ||\\||\\
medical procedures that are performed in, a designated ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
area that is kept free of all vegetative microbes,
||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
endospores, & viruses ||\\||\\ ||\\||\\
Bunsen Burner ||\\||\\
metal apparatus that creates a flame that can be used to
||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
sterilize pieces of equipment ||\\||\\ ||\\||\\ ||\\||\\
BSL-2
- PPE (gloves, coat, & eye protection)
||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
- Sinks & eye wash stations
||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
- restricted access to the lab for authorized persons only
||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
- autoclaves or alternative method(s) of decontamination
||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\ ||\\||\\
BSL-1
