Introduction & Microscopy
August 15th 2024
a mount
Making
1) put cells or tissue onto microscope
slip
2) slide in a drop water
of
cover
~ I
3) lower cover slip
. .be I
ice drop of water
4) avoids creating bubbles
Staining
to help make colorless & white structures visible
-
stain-pigmentthat binds to certain chemical
!!!!
Measuring sizes using eyepiece
graticule
Graticule -
graduated scale placed in eyepiece microscope
of
like ruler to measure the length
Image
Size
actual
Imm 1000 =
mm Magnification
size
always give answer in same unit that
the
question is given in
1um 1000
=
nm
Organelle -
a subcellar structure that
has one or more specific
job to perform in a cell
, The two types Microscope
of
prical (light) microscope
+use to form Image
light
+limits resolution
+
wavelength visible 500-650
light
maximum resolution
+
is 200mm or x1500
used for
-
eukaryotic cells
can't
I
be used to observe smaller organelles
Electron Microscope
use electrons form an
image
+
to
+
More detailedImage
can distinguish somethings really together
+
close
+
maximum of 0.2 mm (1000 greater than optical microscope) 1500,000
x
I can absorb ribsomes
+ two types electron
of microscopes:
Transmission electron microscopes Scanning electron microscopes
(TEM) (SEM)
*
use electromagnet to focus a beam of Scans
-
beam of electron
electron across the specimen
transmit through
+
specimen Beam
+
bounce off surface of
specimen forming an image
denserparts absorb more electrons
+
darker than other parts
- 3D of surface
shows image
+
Advantage Disadv Advantages disadv
High resolution only allows thin specimen low resolution
↑
+
+
can live specimen
I
internal structure
+
no
+
live speciment be usedon no
-
Within cell lengthy treatments 3D specimen thick
+
or
+
no colour image specimen
+
no colour
Image
+ 31 can be observed
August 15th 2024
a mount
Making
1) put cells or tissue onto microscope
slip
2) slide in a drop water
of
cover
~ I
3) lower cover slip
. .be I
ice drop of water
4) avoids creating bubbles
Staining
to help make colorless & white structures visible
-
stain-pigmentthat binds to certain chemical
!!!!
Measuring sizes using eyepiece
graticule
Graticule -
graduated scale placed in eyepiece microscope
of
like ruler to measure the length
Image
Size
actual
Imm 1000 =
mm Magnification
size
always give answer in same unit that
the
question is given in
1um 1000
=
nm
Organelle -
a subcellar structure that
has one or more specific
job to perform in a cell
, The two types Microscope
of
prical (light) microscope
+use to form Image
light
+limits resolution
+
wavelength visible 500-650
light
maximum resolution
+
is 200mm or x1500
used for
-
eukaryotic cells
can't
I
be used to observe smaller organelles
Electron Microscope
use electrons form an
image
+
to
+
More detailedImage
can distinguish somethings really together
+
close
+
maximum of 0.2 mm (1000 greater than optical microscope) 1500,000
x
I can absorb ribsomes
+ two types electron
of microscopes:
Transmission electron microscopes Scanning electron microscopes
(TEM) (SEM)
*
use electromagnet to focus a beam of Scans
-
beam of electron
electron across the specimen
transmit through
+
specimen Beam
+
bounce off surface of
specimen forming an image
denserparts absorb more electrons
+
darker than other parts
- 3D of surface
shows image
+
Advantage Disadv Advantages disadv
High resolution only allows thin specimen low resolution
↑
+
+
can live specimen
I
internal structure
+
no
+
live speciment be usedon no
-
Within cell lengthy treatments 3D specimen thick
+
or
+
no colour image specimen
+
no colour
Image
+ 31 can be observed
