Large-Scale Biotech Fermentation UPDATED
ACTUAL QUESTIONS AND CORRECT Answers
What is the purpose of gene To characterize, clone, improve, and express
isolation in molecular biology? specific genes.
What are the large-scale parameters Temperature, pH, and agitation rate.
that affect bioreactor performance?
What is a major challenge in aerobic Properly oxygenating the culture.
fermentation?
What can happen if waste gases are They can change the chemistry of the liquid,
not removed properly during affecting the fermentation process.
fermentation?
What factors determine optimal Volume, product, and host dependency.
growth conditions in bioprocessing?
What is the significance of GMO To prevent the unintended release of genetically
containment in bioreactor design? modified organisms.
What are the stages of microbial Lag, acceleration, log, deceleration, stationary, and
growth? death.
What is the difference between Batch fermentation uses fresh media at the
batch and fed-batch fermentation? beginning and collects product at the end, while
fed-batch fermentation adds nutrients gradually.
What is the typical starting volume 3-10 mL.
for a stock culture in fermentation?
ACTUAL QUESTIONS AND CORRECT Answers
What is the purpose of gene To characterize, clone, improve, and express
isolation in molecular biology? specific genes.
What are the large-scale parameters Temperature, pH, and agitation rate.
that affect bioreactor performance?
What is a major challenge in aerobic Properly oxygenating the culture.
fermentation?
What can happen if waste gases are They can change the chemistry of the liquid,
not removed properly during affecting the fermentation process.
fermentation?
What factors determine optimal Volume, product, and host dependency.
growth conditions in bioprocessing?
What is the significance of GMO To prevent the unintended release of genetically
containment in bioreactor design? modified organisms.
What are the stages of microbial Lag, acceleration, log, deceleration, stationary, and
growth? death.
What is the difference between Batch fermentation uses fresh media at the
batch and fed-batch fermentation? beginning and collects product at the end, while
fed-batch fermentation adds nutrients gradually.
What is the typical starting volume 3-10 mL.
for a stock culture in fermentation?
