MBIO 3401 TTU Exam 2 Exam
Questions & Answers (Grade A+)
PAM site -
correct answer ✅is a short DNA sequence (usually 2-6 base pairs
in length) that follows the DNA region targeted for cleavage by the
CRISPR system
Shuttle Vector -
correct answer ✅A vector that can propagate between two host
species
DNA ladder(gel electrophoresis) -
correct answer ✅a DNA sequence you already know the
lengths(kDa) to, this is used to reference the size of the unknown
DNA fragments in gel electro
If you ran DNA samples on an electrophoresis gel and forgot to add
a ladder, then __________. -
correct answer ✅The gel electro will run, but it'll be impossible to
distinguish the true size of the unknown DNA because you won't
have a reference.
Heterologous gene -
correct answer ✅refers to the expression of a gene or part of a
gene in a host organism that does not naturally have the gene or
,MBIO 3401 TTU Exam 2 Exam
Questions & Answers (Grade A+)
gene fragment in question. Insertion of the gene in the
heterologous host is performed by recombinant DNA technology.
An example of heterologous expression is __________. -
correct answer ✅a bacterium expressing a human insulin gene
The difference between Southern blotting and Northern blotting is
that __________. -
correct answer ✅Southern blotting uses a DNA target sequence
and Northern blotting uses an RNA target sequence
You create a plasmid with an ampicillin gene that contains multiple
cloning sites (MCSs), then combine it with a restriction enzyme and
DNA fragments cut with the same enzyme. When you insert the
plasmid into bacteria and allow them to grow, ________. -
correct answer ✅bacteria that took up the DNA of interest will be
sensitive to ampicillin
To reduce the public health risks of mosquitoes, researchers are
trying to develop approaches to mosquito control using
__________. -
correct answer ✅gene drives and CRISPR/Cas9
, MBIO 3401 TTU Exam 2 Exam
Questions & Answers (Grade A+)
Genetically engineered organisms, which contain at least one gene
from a different organism, are __________. -
correct answer ✅Transgenic
To insert a piece of DNA into a plasmid, you need to __________. -
correct answer ✅cut the DNA of interest and the plasmid with the
same restriction enzyme to produce complementary sticky ends
Quorum sensing -
correct answer ✅Producing product at specific time
Plants with the Bt transgene are __________. -
correct answer ✅resistant to insects
DNA synthesis during polymerase chain reaction (PCR) differs from
natural DNA synthesis in that __________. -
correct answer ✅DNA primers are used (not RNA primers), and
Thermostable DNA polymerases are used
Heterologous expression -
correct answer ✅obtaining complementary DNA or RNA that helps
you generate proteins that you may not usually make (Like a
Questions & Answers (Grade A+)
PAM site -
correct answer ✅is a short DNA sequence (usually 2-6 base pairs
in length) that follows the DNA region targeted for cleavage by the
CRISPR system
Shuttle Vector -
correct answer ✅A vector that can propagate between two host
species
DNA ladder(gel electrophoresis) -
correct answer ✅a DNA sequence you already know the
lengths(kDa) to, this is used to reference the size of the unknown
DNA fragments in gel electro
If you ran DNA samples on an electrophoresis gel and forgot to add
a ladder, then __________. -
correct answer ✅The gel electro will run, but it'll be impossible to
distinguish the true size of the unknown DNA because you won't
have a reference.
Heterologous gene -
correct answer ✅refers to the expression of a gene or part of a
gene in a host organism that does not naturally have the gene or
,MBIO 3401 TTU Exam 2 Exam
Questions & Answers (Grade A+)
gene fragment in question. Insertion of the gene in the
heterologous host is performed by recombinant DNA technology.
An example of heterologous expression is __________. -
correct answer ✅a bacterium expressing a human insulin gene
The difference between Southern blotting and Northern blotting is
that __________. -
correct answer ✅Southern blotting uses a DNA target sequence
and Northern blotting uses an RNA target sequence
You create a plasmid with an ampicillin gene that contains multiple
cloning sites (MCSs), then combine it with a restriction enzyme and
DNA fragments cut with the same enzyme. When you insert the
plasmid into bacteria and allow them to grow, ________. -
correct answer ✅bacteria that took up the DNA of interest will be
sensitive to ampicillin
To reduce the public health risks of mosquitoes, researchers are
trying to develop approaches to mosquito control using
__________. -
correct answer ✅gene drives and CRISPR/Cas9
, MBIO 3401 TTU Exam 2 Exam
Questions & Answers (Grade A+)
Genetically engineered organisms, which contain at least one gene
from a different organism, are __________. -
correct answer ✅Transgenic
To insert a piece of DNA into a plasmid, you need to __________. -
correct answer ✅cut the DNA of interest and the plasmid with the
same restriction enzyme to produce complementary sticky ends
Quorum sensing -
correct answer ✅Producing product at specific time
Plants with the Bt transgene are __________. -
correct answer ✅resistant to insects
DNA synthesis during polymerase chain reaction (PCR) differs from
natural DNA synthesis in that __________. -
correct answer ✅DNA primers are used (not RNA primers), and
Thermostable DNA polymerases are used
Heterologous expression -
correct answer ✅obtaining complementary DNA or RNA that helps
you generate proteins that you may not usually make (Like a
