GCD 3022 EXAM 4 QUESTIONS AND
CORRECT ANSWERS
\.Which of the following is NOT due to a position effect? - ANSWERS-A
change in gene expression due to a down promoter mutation
\.Nitrous acid replaces amino acid groups with keto groups via a process
called - ANSWERS-Deamination
\.EMS (ethyl methanesulfonate) causes base substitutions by ( ) bases. -
ANSWERS-alkylating
\.A mutation that is a base analog is - ANSWERS-5'-bromouracil
\.Proflavin ( ), creating frameshift mutations. - ANSWERS-intercalates
within DNA helix
(Acridine dyes are also intercalators)
\.What is the mechanism by which UV-rays induce mutations? -
ANSWERS-Creating thymine dimers
, \.(Comprehension Q) Methylated cytosine bases tend to produce
hotspots for spontaneous mutations. Which of the following mutations
is typically observed at a C:G base pair in which the cytosine is
methylated? - ANSWERS-C:G > T:A
\.The function of photolyase is to repair - ANSWERS-thymine dimers
\.The function of alkyltransferase is to repair - ANSWERS-alkylated
guanines
\.Which of the following enzymes recognizes abnormal base structures
during base excision repair? - ANSWERS-DNA N-glycosylases
\.Which of the following enzyme is involved in the step that synthesizes
a new DNA strand in base excision repair in bacteria? - ANSWERS-DNA
polymerase I
\.Which of the following enzymes works together with flap
endonuclease in the step that synthesizes normal DNA during base
excision repair in eukaryotes? - ANSWERS-DNA polymerase delta
(/epsilon?)
\.Which of the following enzymes recognizes thymine dimers during
nucleotide excision repair? - ANSWERS-The UvrA/UvrB complex
CORRECT ANSWERS
\.Which of the following is NOT due to a position effect? - ANSWERS-A
change in gene expression due to a down promoter mutation
\.Nitrous acid replaces amino acid groups with keto groups via a process
called - ANSWERS-Deamination
\.EMS (ethyl methanesulfonate) causes base substitutions by ( ) bases. -
ANSWERS-alkylating
\.A mutation that is a base analog is - ANSWERS-5'-bromouracil
\.Proflavin ( ), creating frameshift mutations. - ANSWERS-intercalates
within DNA helix
(Acridine dyes are also intercalators)
\.What is the mechanism by which UV-rays induce mutations? -
ANSWERS-Creating thymine dimers
, \.(Comprehension Q) Methylated cytosine bases tend to produce
hotspots for spontaneous mutations. Which of the following mutations
is typically observed at a C:G base pair in which the cytosine is
methylated? - ANSWERS-C:G > T:A
\.The function of photolyase is to repair - ANSWERS-thymine dimers
\.The function of alkyltransferase is to repair - ANSWERS-alkylated
guanines
\.Which of the following enzymes recognizes abnormal base structures
during base excision repair? - ANSWERS-DNA N-glycosylases
\.Which of the following enzyme is involved in the step that synthesizes
a new DNA strand in base excision repair in bacteria? - ANSWERS-DNA
polymerase I
\.Which of the following enzymes works together with flap
endonuclease in the step that synthesizes normal DNA during base
excision repair in eukaryotes? - ANSWERS-DNA polymerase delta
(/epsilon?)
\.Which of the following enzymes recognizes thymine dimers during
nucleotide excision repair? - ANSWERS-The UvrA/UvrB complex
