MBIO 3400 Tice Exam 1: CH 4 and 5
Questions & Answers (Grade A+)
What is microscopy? -
correct answer ✅The use of microscopes to view objects too small
to see with the naked eye.
What are the three most important factors in microscopy? -
correct answer ✅Magnification, resolution, and contrast.
What is magnification? -
correct answer ✅How much larger an object appears compared to
its actual size.
What is resolution? -
correct answer ✅The ability to distinguish two objects that are
close together.
Why is resolution more important than magnification? -
correct answer ✅Without good resolution, increased
magnification only makes the image blurry.
How does wavelength affect resolution? -
correct answer ✅Shorter wavelengths produce better resolution.
,MBIO 3400 Tice Exam 1: CH 4 and 5
Questions & Answers (Grade A+)
Why do electron microscopes have higher resolution than light
microscopes? -
correct answer ✅Electrons have much shorter wavelengths than
visible light.
What is the resolution limit of a light microscope? -
correct answer ✅About 200 nanometers.
Why is immersion oil used with the 100× objective lens? -
correct answer ✅It reduces light refraction and improves
resolution.
What happens if immersion oil is not used correctly? -
correct answer ✅Image clarity and resolution decrease.
What is contrast in microscopy? -
correct answer ✅The difference in brightness between an object
and its background.
Why are stains commonly used in microscopy? -
correct answer ✅Most microorganisms are colorless and hard to
see.
, MBIO 3400 Tice Exam 1: CH 4 and 5
Questions & Answers (Grade A+)
When is bright-field microscopy most useful? -
correct answer ✅When specimens are stained.
Why is dark-field useful? -
correct answer ✅It allows viewing of live, unstained cells.
What is the main advantage of phase-contrast microscopy? -
correct answer ✅It reveals internal cell structures without
staining.
How does fluorescence microscopy work? -
correct answer ✅UV light excites fluorescent dyes, which emit
visible light.
What is immunofluorescence used for? -
correct answer ✅Detecting specific microbes or proteins using
antibodies.
Why can electron microscopes resolve smaller objects than light
microscopes? -
correct answer ✅Electrons have shorter wavelengths than light.
Questions & Answers (Grade A+)
What is microscopy? -
correct answer ✅The use of microscopes to view objects too small
to see with the naked eye.
What are the three most important factors in microscopy? -
correct answer ✅Magnification, resolution, and contrast.
What is magnification? -
correct answer ✅How much larger an object appears compared to
its actual size.
What is resolution? -
correct answer ✅The ability to distinguish two objects that are
close together.
Why is resolution more important than magnification? -
correct answer ✅Without good resolution, increased
magnification only makes the image blurry.
How does wavelength affect resolution? -
correct answer ✅Shorter wavelengths produce better resolution.
,MBIO 3400 Tice Exam 1: CH 4 and 5
Questions & Answers (Grade A+)
Why do electron microscopes have higher resolution than light
microscopes? -
correct answer ✅Electrons have much shorter wavelengths than
visible light.
What is the resolution limit of a light microscope? -
correct answer ✅About 200 nanometers.
Why is immersion oil used with the 100× objective lens? -
correct answer ✅It reduces light refraction and improves
resolution.
What happens if immersion oil is not used correctly? -
correct answer ✅Image clarity and resolution decrease.
What is contrast in microscopy? -
correct answer ✅The difference in brightness between an object
and its background.
Why are stains commonly used in microscopy? -
correct answer ✅Most microorganisms are colorless and hard to
see.
, MBIO 3400 Tice Exam 1: CH 4 and 5
Questions & Answers (Grade A+)
When is bright-field microscopy most useful? -
correct answer ✅When specimens are stained.
Why is dark-field useful? -
correct answer ✅It allows viewing of live, unstained cells.
What is the main advantage of phase-contrast microscopy? -
correct answer ✅It reveals internal cell structures without
staining.
How does fluorescence microscopy work? -
correct answer ✅UV light excites fluorescent dyes, which emit
visible light.
What is immunofluorescence used for? -
correct answer ✅Detecting specific microbes or proteins using
antibodies.
Why can electron microscopes resolve smaller objects than light
microscopes? -
correct answer ✅Electrons have shorter wavelengths than light.
