MBIO 3400 Alexander Tice Exam 2 pt 1
Questions & Answers (Grade A+)
Which of the following statements is true concerning recombinant
DNA technology?
a. it will replace biotechnology in the future.
b. it is a single technique for genetic manipulation
c. It is useful in manipulating genotypes but not phenotypes.
d. it involves modification of an organism's genome. -
correct answer ✅D. it involves modification of an organism's
genome.
A DNA gene synthesized from an RNA template is ___________.
A. Reverse transcriptase
B. Complementary DNA
C. Recombinant DNA
D. Probe DNA -
correct answer ✅b. complementary DNA
After Scientists exposed cultures of Penicillium to agents X, Y, and Z,
there examined the type and amount of penicillin produced by the
altered fungi to find the one that is most effective. Agents X, Y, and
Z were probably ___________.
,MBIO 3400 Alexander Tice Exam 2 pt 1
Questions & Answers (Grade A+)
a. recombinant cells
b. competent
c. mutagens
d. phages -
correct answer ✅c. mutagens
which of the following is false concerning vectors in recombinant
DNA technology?
a. vectors are small enough to manipulate outside a cell.
b. vectors contain a recognizable genetic marker.
c. vectors survive inside cells.
d. vectors must contain genes for self-replication. -
correct answer ✅D. Vectors must contain genes for self-
replication.
Which recombinant DNA technique is used to replicate copies of a
DNA molecule?
a. PCR
,MBIO 3400 Alexander Tice Exam 2 pt 1
Questions & Answers (Grade A+)
b. gel electrophoresis
c. electroporation
d. reverse transcription -
correct answer ✅PCR
Which of the following would've most useful in following gene
expression in a yeast cell?
a. southern blot
b. reverse transcription
c. DNA microarray
d. restriction enzymes -
correct answer ✅c. DNA microarray
which of the following techniques is used regularly in the study of
genomics?
a. clones are selected using a vector with two genetic markers
b. genes are inserted to produce an antigenic protein from a
pathogen
c. fluorescent nucleotide bases are sequenced
, MBIO 3400 Alexander Tice Exam 2 pt 1
Questions & Answers (Grade A+)
d. defective organs are replaced with those made in animal hosts -
correct answer ✅c. Fluorescent nucleotide bases are sequenced
restriction enzyme HhaI ____________
a. recombines DNA
b. cuts DNA at a specific nucleotide sequence
c. is likely derived from Haemophilus influenzae
d. all of the above are correct -
correct answer ✅b. cuts DNA at a specific nucleotide sequence
Which application of recombinant DNA technology involves
replacing a nonfunctional, mutated gene with a function gene
a. gene therapy
b. functional genomics
c. genetic screening
d. protein synthesis -
correct answer ✅a. gene therapy
A DNA microarray consists of _________________.
Questions & Answers (Grade A+)
Which of the following statements is true concerning recombinant
DNA technology?
a. it will replace biotechnology in the future.
b. it is a single technique for genetic manipulation
c. It is useful in manipulating genotypes but not phenotypes.
d. it involves modification of an organism's genome. -
correct answer ✅D. it involves modification of an organism's
genome.
A DNA gene synthesized from an RNA template is ___________.
A. Reverse transcriptase
B. Complementary DNA
C. Recombinant DNA
D. Probe DNA -
correct answer ✅b. complementary DNA
After Scientists exposed cultures of Penicillium to agents X, Y, and Z,
there examined the type and amount of penicillin produced by the
altered fungi to find the one that is most effective. Agents X, Y, and
Z were probably ___________.
,MBIO 3400 Alexander Tice Exam 2 pt 1
Questions & Answers (Grade A+)
a. recombinant cells
b. competent
c. mutagens
d. phages -
correct answer ✅c. mutagens
which of the following is false concerning vectors in recombinant
DNA technology?
a. vectors are small enough to manipulate outside a cell.
b. vectors contain a recognizable genetic marker.
c. vectors survive inside cells.
d. vectors must contain genes for self-replication. -
correct answer ✅D. Vectors must contain genes for self-
replication.
Which recombinant DNA technique is used to replicate copies of a
DNA molecule?
a. PCR
,MBIO 3400 Alexander Tice Exam 2 pt 1
Questions & Answers (Grade A+)
b. gel electrophoresis
c. electroporation
d. reverse transcription -
correct answer ✅PCR
Which of the following would've most useful in following gene
expression in a yeast cell?
a. southern blot
b. reverse transcription
c. DNA microarray
d. restriction enzymes -
correct answer ✅c. DNA microarray
which of the following techniques is used regularly in the study of
genomics?
a. clones are selected using a vector with two genetic markers
b. genes are inserted to produce an antigenic protein from a
pathogen
c. fluorescent nucleotide bases are sequenced
, MBIO 3400 Alexander Tice Exam 2 pt 1
Questions & Answers (Grade A+)
d. defective organs are replaced with those made in animal hosts -
correct answer ✅c. Fluorescent nucleotide bases are sequenced
restriction enzyme HhaI ____________
a. recombines DNA
b. cuts DNA at a specific nucleotide sequence
c. is likely derived from Haemophilus influenzae
d. all of the above are correct -
correct answer ✅b. cuts DNA at a specific nucleotide sequence
Which application of recombinant DNA technology involves
replacing a nonfunctional, mutated gene with a function gene
a. gene therapy
b. functional genomics
c. genetic screening
d. protein synthesis -
correct answer ✅a. gene therapy
A DNA microarray consists of _________________.
