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MBIO 3401 Exam 2 Review Questions & Answers (Grade A+).docx

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MBIO 3401 Exam 2 Review Questions & Answers (Grade A+).docx

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MBIO 3401 Exam 2 Review Questions
& Answers (Grade A+)
Phase variation of flagellar proteins in Salmonella enterica involves
-
correct answer ✅DNA rearrangements.


Transcription is prevented by _______, and stimulated by _______.
-
correct answer ✅repressors; activators


What is the phenotype of an Escherichia coli strain with a mutation
in the lac operator that prevents the lac repressor from binding?
Assume glucose is absent. -
correct answer ✅Transcription of the lac operon is very high


Transcription initiation of the lac operon is enhanced when
_______ interacts with RNA polymerase -
correct answer ✅cAMP receptor protein


Attenuation is a common regulatory strategy used to control the
transcription of operons that code for -
correct answer ✅amino acid biosynthesis.

,MBIO 3401 Exam 2 Review Questions
& Answers (Grade A+)
Which of the following can be used to control sigma factors? -
correct answer ✅a) Proteolysis
b) Anti-sigma factors
c) Anti-anti-sigma factors
d) Two of the above
e) All of the above


_______ RNAs bind to complementary sequences of target
transcripts and stimulate or prevent translation. -
correct answer ✅Antisense


Methyl-accepting chemotaxis proteins (MCPs) -
correct answer ✅bind attractants from the environment.


A quorum-sensing gene system requires the accumulation of a
secreted small molecule called -
correct answer ✅an autoinducer.


Two-dimensional gels are used to directly study the -
correct answer ✅proteome.

, MBIO 3401 Exam 2 Review Questions
& Answers (Grade A+)
Predict what would happen if you generated a mutant
bacteriophage lambda that expressed lower levels of the CI
repressor -
correct answer ✅b) The phage would exhibit higher levels of lytic
cycle activation.
c) The host cell would be more susceptible to superinfection by
other lambda phages


Bacteriophage lambda binds to the ______ that is embedded in the
outer membrane of E. coli -
correct answer ✅maltose porin


Upon infection of a host cell, bacteriophage lambda -
correct answer ✅enters a lysogenic cycle exclusively


Why is it necessary for influenza to package an RNA-dependent
RNA polymerase within its segmented genome? -
correct answer ✅a) Negative strand RNA cannot serve as mRNA
directly.
b) Human cells to not contain a native RNA-dependent RNA
polymerase.

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