MBIO 3401 Lab Final Exam Questions & Answers (Grade A+).docx

MBIO 3401 Lab Final Exam Questions
& Answers (Grade A+)
What is the purpose of the IMViC Test? -
correct answer ✅This test is used to identify enteric bacteria such
as pathogens.


What are the 4 different tests for the IMViC test? -
correct answer ✅1. Indole Production Test
2. Methyl Red Test
3. Voges-Proskaeur Test
4. Citrate utilization test


Indole Production Test -
correct answer ✅Tests an organism ability to produce the enzyme
tryptophanase and hydrolyze tryptophan into indole and pyruvate.


Positive Test: Produces a rose color when reacting with indole.
Negative Test: Degrades the indole to.


Methyl Red Test -
correct answer ✅Asses ability of organism to produce a mixed acid
fermentation.

, MBIO 3401 Lab Final Exam Questions
& Answers (Grade A+)
Positive: Red Color, bacteria can ferment glucose into stable acids.


Negative: Yellow color, detects production of acetoin from
fermentation of glucose.


Voges-Proskauer Test -
correct answer ✅Detects the production of acetoin from
fermentation of glucose.


Positive: Red Color after addition of reagents.


Negative: No color change, suggesting organism either didn't
produce acetoin or low quantity.


Citrate Test -
correct answer ✅Used to determine ability of organism to use
citrate as sole carbon source for growth. Helps differentiate and ID
bacteria within Enterobacteriaceae family.


Positive: Organism capable of utilizing citrate as carbon source. Will
bubble up.