BIOTECH EXAM 3 STUDY GUIDE, KEY CONCEPTS &
APPLICATIONS LATEST REVIEW 2026
Question 1
0..5 pts
The term "tissue culture" could include
culture of animal cells
culture of tissue fragments
culture of plant tissues
culture of organs
Correct answer
Question 2
0..5 pts
The incubator of cell culture should be able to control
humidity
CO2 concentration
pressure
temperature
Correct answer
Question 3
pts
Why do cells need to be subcultured after reaching confluence?
To reduce the nutrient content in the culture medium
To stop their growth and preserve them
To change their genetic makeup
To provide more room for continued growth by transferring them to a new vessel
Correct answer
Question 4
0..5 pts
, What is true about cell lines derived from primary cultures?
They cannot be passaged further
They are finite and have a limited life span
They lose all genetic variability
They have an unlimited life span
Correct answer
Question 5
pts
How is a cell strain created?
By positively selecting a subpopulation from a cell line through cloning or other methods
By mixing multiple cell lines together
By exposing cells to radiation
By freezing a cell line for long-term storage
Correct answer
Question 6
pts
What is true about cell strains compared to their parent cell lines?
They cannot be used in experiments
They lose all genetic variability
They are genetically identical to the parent line
They often acquire additional genetic changes
Correct answer
Question 7
pts
How do finite cell lines become continuous cell lines?
By reducing the nutrient content in the culture medium
By increasing the temperature of the culture environment
By entering senescence
APPLICATIONS LATEST REVIEW 2026
Question 1
0..5 pts
The term "tissue culture" could include
culture of animal cells
culture of tissue fragments
culture of plant tissues
culture of organs
Correct answer
Question 2
0..5 pts
The incubator of cell culture should be able to control
humidity
CO2 concentration
pressure
temperature
Correct answer
Question 3
pts
Why do cells need to be subcultured after reaching confluence?
To reduce the nutrient content in the culture medium
To stop their growth and preserve them
To change their genetic makeup
To provide more room for continued growth by transferring them to a new vessel
Correct answer
Question 4
0..5 pts
, What is true about cell lines derived from primary cultures?
They cannot be passaged further
They are finite and have a limited life span
They lose all genetic variability
They have an unlimited life span
Correct answer
Question 5
pts
How is a cell strain created?
By positively selecting a subpopulation from a cell line through cloning or other methods
By mixing multiple cell lines together
By exposing cells to radiation
By freezing a cell line for long-term storage
Correct answer
Question 6
pts
What is true about cell strains compared to their parent cell lines?
They cannot be used in experiments
They lose all genetic variability
They are genetically identical to the parent line
They often acquire additional genetic changes
Correct answer
Question 7
pts
How do finite cell lines become continuous cell lines?
By reducing the nutrient content in the culture medium
By increasing the temperature of the culture environment
By entering senescence
