Hartman Phlebotomy Practice Exam
Questions and Answers
Comprehensive Certification Review
Official Hartman Publishing Phlebotomy Certification Practice Test
Blueprint Replica – 150 Questions
Aligned with NHA/ASCP/AMCA Certification Standards
HARTMAN PHLEBOTOMY PRACTICE EXAM
Official Hartman Publishing Phlebotomy Certification Practice Test Blueprint Replica – 150
Questions
Aligned with NHA/ASCP/AMCA Certification Standards
SECTION 1: CIRCULATORY SYSTEM & BLOOD COMPOSITION (Questions 1–
20)
Q1: Which chamber of the heart receives deoxygenated blood from the superior and inferior
vena cava?
• A. Left atrium
• B. Left ventricle
• C. Right atrium [CORRECT]
• D. Right ventricle
Correct Answer: C
Rationale: Correct because Hartman's Chapter 2 explains that the right atrium receives
deoxygenated blood returning from the body via the superior vena cava (from above the heart)
and inferior vena cava (from below the heart) before passing it through the tricuspid valve to the
right ventricle.
,Q2: Blood flows from the right ventricle through which valve into the pulmonary artery?
• A. Tricuspid valve
• B. Mitral valve
• C. Pulmonary semilunar valve [CORRECT]
• D. Aortic semilunar valve
Correct Answer: C
Rationale: Correct because Hartman's Chapter 2 states that the pulmonary semilunar valve
prevents backflow from the pulmonary artery into the right ventricle as deoxygenated blood is
pumped to the lungs for gas exchange.
Q3: Which valve is located between the left atrium and left ventricle?
• A. Tricuspid valve
• B. Pulmonary semilunar valve
• C. Mitral (bicuspid) valve [CORRECT]
• D. Aortic semilunar valve
Correct Answer: C
Rationale: Correct because Hartman's Chapter 2 identifies the mitral or bicuspid valve as the
atrioventricular valve on the left side of the heart, named for its two cusps, which closes during
ventricular systole to prevent backflow into the left atrium.
Q4: Which blood vessel carries oxygenated blood from the lungs to the left atrium?
• A. Pulmonary artery
• B. Superior vena cava
• C. Pulmonary veins [CORRECT]
• D. Inferior vena cava
Correct Answer: C
Rationale: Correct because Hartman's Chapter 2 explains that pulmonary veins are the only veins
in the body that carry oxygenated blood, returning freshly oxygenated blood from the lungs to
the left atrium of the heart.
,Q5: Which of the following correctly describes the complete pathway of blood through the
heart and lungs?
• A. SVC/IVC → LA → mitral → LV → aortic valve → aorta → body
• B. SVC/IVC → RA → tricuspid → RV → pulmonary valve → pulmonary artery → lungs →
pulmonary veins → LA → mitral → LV → aortic valve → aorta → body [CORRECT]
• C. SVC/IVC → RA → mitral → RV → pulmonary valve → pulmonary artery → lungs →
pulmonary veins → LA → tricuspid → LV → aortic valve → aorta → body
• D. SVC/IVC → LA → tricuspid → LV → pulmonary valve → pulmonary artery → lungs →
pulmonary veins → RA → mitral → RV → aortic valve → aorta → body
Correct Answer: B
Rationale: Correct because Hartman's Chapter 2 presents the complete blood flow pathway:
deoxygenated blood enters the right atrium, passes through the tricuspid valve to the right
ventricle, is pumped through the pulmonary valve to the pulmonary artery to the lungs for
oxygenation, returns via pulmonary veins to the left atrium, passes through the mitral valve to
the left ventricle, and is pumped through the aortic valve to the aorta for systemic circulation.
Q6: Which structural feature distinguishes arteries from veins?
• A. Veins have thicker muscular walls than arteries
• B. Arteries carry blood toward the heart; veins carry blood away from the heart
• C. Arteries have thick muscular walls and carry blood away from the heart; veins have
thinner walls, contain valves, and carry blood toward the heart [CORRECT]
• D. Capillaries connect arteries to other arteries
Correct Answer: C
Rationale: Correct because Hartman's Chapter 2 explains that arteries have thick, elastic,
muscular walls to withstand higher pressure as they carry blood away from the heart, while
veins have thinner walls with valves to prevent backflow as they return blood to the heart under
lower pressure.
Q7: Capillaries are best described as:
• A. Large vessels with thick muscular walls that carry blood away from the heart
• B. Vessels with valves that return blood to the heart
• C. Microscopic vessels that are one cell thick, allowing exchange of oxygen, carbon
dioxide, nutrients, and waste between blood and tissues [CORRECT]
• D. Vessels that connect the pulmonary artery directly to the aorta
, Correct Answer: C
Rationale: Correct because Hartman's Chapter 2 states that capillaries are the smallest blood
vessels, composed of a single layer of endothelial cells, where the critical exchange of gases,
nutrients, and waste products occurs between the bloodstream and body tissues.
Q8: Which blood cell is responsible for transporting oxygen throughout the body?
• A. Leukocyte
• B. Erythrocyte [CORRECT]
• C. Thrombocyte
• D. Lymphocyte
Correct Answer: B
Rationale: Correct because Hartman's Chapter 3 explains that erythrocytes, or red blood cells
(RBCs), contain hemoglobin which binds oxygen in the lungs and releases it to tissues; normal
RBC count is approximately 4.5–6.0 million/mm3 in adults.
Q9: What is the normal hemoglobin range for an adult male?
• A. 8–10 g/dL
• B. 10–12 g/dL
• C. 12–18 g/dL [CORRECT]
• D. 18–22 g/dL
Correct Answer: C
Rationale: Correct because Hartman's Chapter 3 states that normal hemoglobin levels range
from approximately 12–18 g/dL, with males typically at the higher end (14–18 g/dL) and females
at the lower end (12–16 g/dL), reflecting the oxygen-carrying capacity of the blood.
Q10: Which type of white blood cell constitutes 60–70% of the total WBC count and is the first
responder to bacterial infection?
• A. Eosinophil
• B. Basophil
• C. Neutrophil [CORRECT]
• D. Lymphocyte
Questions and Answers
Comprehensive Certification Review
Official Hartman Publishing Phlebotomy Certification Practice Test
Blueprint Replica – 150 Questions
Aligned with NHA/ASCP/AMCA Certification Standards
HARTMAN PHLEBOTOMY PRACTICE EXAM
Official Hartman Publishing Phlebotomy Certification Practice Test Blueprint Replica – 150
Questions
Aligned with NHA/ASCP/AMCA Certification Standards
SECTION 1: CIRCULATORY SYSTEM & BLOOD COMPOSITION (Questions 1–
20)
Q1: Which chamber of the heart receives deoxygenated blood from the superior and inferior
vena cava?
• A. Left atrium
• B. Left ventricle
• C. Right atrium [CORRECT]
• D. Right ventricle
Correct Answer: C
Rationale: Correct because Hartman's Chapter 2 explains that the right atrium receives
deoxygenated blood returning from the body via the superior vena cava (from above the heart)
and inferior vena cava (from below the heart) before passing it through the tricuspid valve to the
right ventricle.
,Q2: Blood flows from the right ventricle through which valve into the pulmonary artery?
• A. Tricuspid valve
• B. Mitral valve
• C. Pulmonary semilunar valve [CORRECT]
• D. Aortic semilunar valve
Correct Answer: C
Rationale: Correct because Hartman's Chapter 2 states that the pulmonary semilunar valve
prevents backflow from the pulmonary artery into the right ventricle as deoxygenated blood is
pumped to the lungs for gas exchange.
Q3: Which valve is located between the left atrium and left ventricle?
• A. Tricuspid valve
• B. Pulmonary semilunar valve
• C. Mitral (bicuspid) valve [CORRECT]
• D. Aortic semilunar valve
Correct Answer: C
Rationale: Correct because Hartman's Chapter 2 identifies the mitral or bicuspid valve as the
atrioventricular valve on the left side of the heart, named for its two cusps, which closes during
ventricular systole to prevent backflow into the left atrium.
Q4: Which blood vessel carries oxygenated blood from the lungs to the left atrium?
• A. Pulmonary artery
• B. Superior vena cava
• C. Pulmonary veins [CORRECT]
• D. Inferior vena cava
Correct Answer: C
Rationale: Correct because Hartman's Chapter 2 explains that pulmonary veins are the only veins
in the body that carry oxygenated blood, returning freshly oxygenated blood from the lungs to
the left atrium of the heart.
,Q5: Which of the following correctly describes the complete pathway of blood through the
heart and lungs?
• A. SVC/IVC → LA → mitral → LV → aortic valve → aorta → body
• B. SVC/IVC → RA → tricuspid → RV → pulmonary valve → pulmonary artery → lungs →
pulmonary veins → LA → mitral → LV → aortic valve → aorta → body [CORRECT]
• C. SVC/IVC → RA → mitral → RV → pulmonary valve → pulmonary artery → lungs →
pulmonary veins → LA → tricuspid → LV → aortic valve → aorta → body
• D. SVC/IVC → LA → tricuspid → LV → pulmonary valve → pulmonary artery → lungs →
pulmonary veins → RA → mitral → RV → aortic valve → aorta → body
Correct Answer: B
Rationale: Correct because Hartman's Chapter 2 presents the complete blood flow pathway:
deoxygenated blood enters the right atrium, passes through the tricuspid valve to the right
ventricle, is pumped through the pulmonary valve to the pulmonary artery to the lungs for
oxygenation, returns via pulmonary veins to the left atrium, passes through the mitral valve to
the left ventricle, and is pumped through the aortic valve to the aorta for systemic circulation.
Q6: Which structural feature distinguishes arteries from veins?
• A. Veins have thicker muscular walls than arteries
• B. Arteries carry blood toward the heart; veins carry blood away from the heart
• C. Arteries have thick muscular walls and carry blood away from the heart; veins have
thinner walls, contain valves, and carry blood toward the heart [CORRECT]
• D. Capillaries connect arteries to other arteries
Correct Answer: C
Rationale: Correct because Hartman's Chapter 2 explains that arteries have thick, elastic,
muscular walls to withstand higher pressure as they carry blood away from the heart, while
veins have thinner walls with valves to prevent backflow as they return blood to the heart under
lower pressure.
Q7: Capillaries are best described as:
• A. Large vessels with thick muscular walls that carry blood away from the heart
• B. Vessels with valves that return blood to the heart
• C. Microscopic vessels that are one cell thick, allowing exchange of oxygen, carbon
dioxide, nutrients, and waste between blood and tissues [CORRECT]
• D. Vessels that connect the pulmonary artery directly to the aorta
, Correct Answer: C
Rationale: Correct because Hartman's Chapter 2 states that capillaries are the smallest blood
vessels, composed of a single layer of endothelial cells, where the critical exchange of gases,
nutrients, and waste products occurs between the bloodstream and body tissues.
Q8: Which blood cell is responsible for transporting oxygen throughout the body?
• A. Leukocyte
• B. Erythrocyte [CORRECT]
• C. Thrombocyte
• D. Lymphocyte
Correct Answer: B
Rationale: Correct because Hartman's Chapter 3 explains that erythrocytes, or red blood cells
(RBCs), contain hemoglobin which binds oxygen in the lungs and releases it to tissues; normal
RBC count is approximately 4.5–6.0 million/mm3 in adults.
Q9: What is the normal hemoglobin range for an adult male?
• A. 8–10 g/dL
• B. 10–12 g/dL
• C. 12–18 g/dL [CORRECT]
• D. 18–22 g/dL
Correct Answer: C
Rationale: Correct because Hartman's Chapter 3 states that normal hemoglobin levels range
from approximately 12–18 g/dL, with males typically at the higher end (14–18 g/dL) and females
at the lower end (12–16 g/dL), reflecting the oxygen-carrying capacity of the blood.
Q10: Which type of white blood cell constitutes 60–70% of the total WBC count and is the first
responder to bacterial infection?
• A. Eosinophil
• B. Basophil
• C. Neutrophil [CORRECT]
• D. Lymphocyte
