2 forms of 30 nm fiber Correct Answers 1. Solenoid model
(low salt, H1 bound, more compact)
2. Zigzag model (moderate salt, H1 removed less compact)
2 multiprotein complexes that form and organize metaphase
chromosomes Correct Answers 1. Condensin (compaction)
2. Cohesin (sister chromatid alignment)
2 parts of the nuclear matrix Correct Answers 1. Nuclear
lamina
2. Internal nuclear matrix
2 types of heterochromatin Correct Answers 1. Constitutive -
always hetero
2. Facultative - can interconvert b/w hetero and euch
2 unusual features of DNA polymerase Correct Answers 1.
Synthesize 5' --> 3'
2. Cannot initiate DNA synthesis
3 types of DNA sequences required for chromosomal replication
and segregation Correct Answers 1. Origins of replication
2. Centromeres (segregation)
3. Telomeres (end of chromosomes, replication and stability)
3 types of functionally significant DNA sequences in oriC
Correct Answers 1. AT-rich region
2. DnaA boxes
3. GATC methylation sites (regulate)
, 30 nm fiber Correct Answers Nucleosome units organized into
a more compact structure that is 30 nm in diameter
A phosphate connects the ___ carbon of one nucleotide to the __
carbon of another Correct Answers 5' to 3'
a-polymerase (eukaryotes) Correct Answers Initiates
replication in conjucation with primase
a-subunit Correct Answers Synthesizes DNA
Avery, MacLeod, McCarty experiments (DNA transformation)
Correct Answers Conducted in the 1940s. Carried out
experiments following Griffith's, and found that IIR was only
converted to IIIS when the DNA was purified and that DNase
was the only substance that affected the transformation process.
This provided evidence that DNA was the genetic material.
B-subunit Correct Answers Clamp protein, allows DNA
polymerase to slide without falling off
Bacteria genetic material is usually a ___ Correct Answers
Single circular chromosome
Chargaff's rule Correct Answers A=T and C=G
Chromatin Correct Answers The DNA-protein complex
Chromosomes Correct Answers Complexes of DNA and
proteins that contain all the genetic material