GCD 3022 - Chapter 20 Questions With Complete Solutions
annealing Correct Answers in PCR, the process in which a
primer binds to a strand of template DNA
antibody Correct Answers protein that are produced by the
immune systems of vertebrates, which recognize foreign
material (namely, viruses, bacteria, and so forth) and target it for
destructions; also known as immunoglobins
basic approach to making a genomic library and a cDNA library
Correct Answers
blunt end Correct Answers Restriction fragments with no
overlapping ends resulting from cleavage by a restriction
enzyme
non-cohesive ends, since there is no unpaired DNA strand
fleeting at the end of DNA
cDNA Correct Answers DNA that is a made from an RNA
template by the action of reverse transcriptase
cDNA library Correct Answers a DNA library who
recombinant vectors carry cDNA inserts
competent cells Correct Answers cells that can take up DNA
from the environment or an extracellular medium
, CRISPR-Cas Correct Answers a method that uses components
of the CRISPR-Cas system of genome defense found in
prokaryotes to introduce mutations into genes
CRISPR-Cas system: a system found in bacteria and archaea
that provides defense against viruses, plasmids, and transposable
elements
dideoxy DNA sequencing Correct Answers a method of DNA
sequencing that uses dideoxyribonucleotides to terminate the
growth of DNA strands
differences between a selectable marker and a screening marker
Correct Answers
DNA ligase Correct Answers an enzyme that catalyzes the
formation of a covalent bond within the sugar-phosphate
backbones of two DNA stands
genomic library Correct Answers a DNA library or
recombinant vectors that carry chromosomal DNA fragments
host cell Correct Answers a cell that is infected with a virus or
bacterium, or one that harbors a vector
how CRISPR-Cas is used to manipulate the genome Correct
Answers
how restriction enzymes can be used to generate recombinant
DNA Correct Answers
annealing Correct Answers in PCR, the process in which a
primer binds to a strand of template DNA
antibody Correct Answers protein that are produced by the
immune systems of vertebrates, which recognize foreign
material (namely, viruses, bacteria, and so forth) and target it for
destructions; also known as immunoglobins
basic approach to making a genomic library and a cDNA library
Correct Answers
blunt end Correct Answers Restriction fragments with no
overlapping ends resulting from cleavage by a restriction
enzyme
non-cohesive ends, since there is no unpaired DNA strand
fleeting at the end of DNA
cDNA Correct Answers DNA that is a made from an RNA
template by the action of reverse transcriptase
cDNA library Correct Answers a DNA library who
recombinant vectors carry cDNA inserts
competent cells Correct Answers cells that can take up DNA
from the environment or an extracellular medium
, CRISPR-Cas Correct Answers a method that uses components
of the CRISPR-Cas system of genome defense found in
prokaryotes to introduce mutations into genes
CRISPR-Cas system: a system found in bacteria and archaea
that provides defense against viruses, plasmids, and transposable
elements
dideoxy DNA sequencing Correct Answers a method of DNA
sequencing that uses dideoxyribonucleotides to terminate the
growth of DNA strands
differences between a selectable marker and a screening marker
Correct Answers
DNA ligase Correct Answers an enzyme that catalyzes the
formation of a covalent bond within the sugar-phosphate
backbones of two DNA stands
genomic library Correct Answers a DNA library or
recombinant vectors that carry chromosomal DNA fragments
host cell Correct Answers a cell that is infected with a virus or
bacterium, or one that harbors a vector
how CRISPR-Cas is used to manipulate the genome Correct
Answers
how restriction enzymes can be used to generate recombinant
DNA Correct Answers
