AMCA PHLEBOTOMY EXAM REVIEW
QUESTIONS – 100% VERIFIED ANSWERS
WITH RATIONALES LATEST UPDATE –
GRADED A+
1. Which of the following is not a phlebotomist’s duty?
Answer: Starting intravenous (IV) lines
Rationale: IV initiation is typically outside the phlebotomist’s scope; phlebotomists
perform venipuncture for blood collection, not IV therapy.
2. A national organization that sets standards for phlebotomy procedures is the:
Answer: Clinical and Laboratory Standards Institute (CLSI)
3. Which type of contact infection transmission involves transfer of an infective
microbe to the mucous membranes of a susceptible individual by means of a cough
or sneeze?
Answer: Droplet transmission
4. Which of the following is a test of the respiratory system?
Answer: Arterial blood gases (ABGs)
5. The receiving chambers of the heart are the:
Answer: Atria
6. An individual’s blood type is determined by the presence or absence of a certain
type of:
Answer: Antigen present on the red blood cell
,7. Which of the following veins is not an antecubital vein?
Answer: Femoral vein
8. The preferred site for adult venipuncture is the:
Answer: Median cubital vein
9. Which additive is found in a light blue top tube?
Answer: Sodium citrate
10. A lavender top tube contains which anticoagulant?
Answer: EDTA (ethylenediaminetetraacetic acid)
11. What is the correct order of draw for capillary specimens?
Answer: Blood gas, lavender, green, gray
12. The tourniquet should be applied for no longer than:
Answer: 1 minute
13. Which needle gauge is most commonly used for routine adult venipuncture?
Answer: 21 gauge
14. A butterfly needle set is typically used for:
Answer: Small or fragile veins
15. Which of the following is an acceptable site for skin puncture on an infant?
Answer: Lateral heel
, 16. What is the preferred angle of insertion for venipuncture using a butterfly
needle?
Answer: 10-15 degrees
17. The first tube drawn in a routine venipuncture should be the:
Answer: Blood culture bottle (sterile first)
18. Which of the following tubes should be drawn after a light blue top tube?
Answer: Serum separator tube (SST) – but standard order of draw: blood
culture, light blue, red/SST, green, lavender, gray.
Answer: Red or serum separator tube
19. A serum separator tube (SST) contains:
Answer: Clot activator and gel
20. Which of the following tests requires a fasting specimen?
Answer: Lipid panel
21. Which vein is the first choice for venipuncture in most patients?
Answer: Median cubital vein
22. The cephalic vein is located on which side of the arm?
Answer: Thumb (lateral) side
23. The basilic vein is located on which side of the arm?
Answer: Little finger (medial) side
QUESTIONS – 100% VERIFIED ANSWERS
WITH RATIONALES LATEST UPDATE –
GRADED A+
1. Which of the following is not a phlebotomist’s duty?
Answer: Starting intravenous (IV) lines
Rationale: IV initiation is typically outside the phlebotomist’s scope; phlebotomists
perform venipuncture for blood collection, not IV therapy.
2. A national organization that sets standards for phlebotomy procedures is the:
Answer: Clinical and Laboratory Standards Institute (CLSI)
3. Which type of contact infection transmission involves transfer of an infective
microbe to the mucous membranes of a susceptible individual by means of a cough
or sneeze?
Answer: Droplet transmission
4. Which of the following is a test of the respiratory system?
Answer: Arterial blood gases (ABGs)
5. The receiving chambers of the heart are the:
Answer: Atria
6. An individual’s blood type is determined by the presence or absence of a certain
type of:
Answer: Antigen present on the red blood cell
,7. Which of the following veins is not an antecubital vein?
Answer: Femoral vein
8. The preferred site for adult venipuncture is the:
Answer: Median cubital vein
9. Which additive is found in a light blue top tube?
Answer: Sodium citrate
10. A lavender top tube contains which anticoagulant?
Answer: EDTA (ethylenediaminetetraacetic acid)
11. What is the correct order of draw for capillary specimens?
Answer: Blood gas, lavender, green, gray
12. The tourniquet should be applied for no longer than:
Answer: 1 minute
13. Which needle gauge is most commonly used for routine adult venipuncture?
Answer: 21 gauge
14. A butterfly needle set is typically used for:
Answer: Small or fragile veins
15. Which of the following is an acceptable site for skin puncture on an infant?
Answer: Lateral heel
, 16. What is the preferred angle of insertion for venipuncture using a butterfly
needle?
Answer: 10-15 degrees
17. The first tube drawn in a routine venipuncture should be the:
Answer: Blood culture bottle (sterile first)
18. Which of the following tubes should be drawn after a light blue top tube?
Answer: Serum separator tube (SST) – but standard order of draw: blood
culture, light blue, red/SST, green, lavender, gray.
Answer: Red or serum separator tube
19. A serum separator tube (SST) contains:
Answer: Clot activator and gel
20. Which of the following tests requires a fasting specimen?
Answer: Lipid panel
21. Which vein is the first choice for venipuncture in most patients?
Answer: Median cubital vein
22. The cephalic vein is located on which side of the arm?
Answer: Thumb (lateral) side
23. The basilic vein is located on which side of the arm?
Answer: Little finger (medial) side
