M118L Quiz 1 Newest 2026 Questions and
Correct Detailed Answers Already Graded A+
Name the media used for minimal media test? Why is it called a defined media? -
CORRECT ANSWER-The media used for the media test (succinate-NH₃ broth)
contains sodium succinate as the carbon source and ammonium chloride as the
nitrogen source. It is called a defined media because we know exactly what is in it;
there are no materials or substances of unknown composition. It is not a rich
media.
What is the purpose of the minimal media test (what is it trying to find out about
your isolate)? Name one bacteria that cannot grow in minimal media? -
CORRECT ANSWER-The purpose of the minimal media test is to see if the
bacteria are able to grow without a source of enrichment. If the media is cloudy
after inoculation, the isolate does not require sugars and a complex medium for
growth.
One bacteria that cannot grow in minimal media is Streptococcus.
Why do we use King's agar B and not S1 for the Fluorescent Pigment Test? -
CORRECT ANSWER-We use King's Agar B plate instead of the S1 plate because
unlike S1, it is not selective and lacks compounds that inhibit the growth of
, certain types of organisms. It additionally contains phosphorus and magnesium,
which has been demonstrated to increase production of the fluorescent pigment
pyoverdin.
Where do you dispose of plastic Petri dishes, swabs, contaminated oxidase paper,
wooden sticks, and other contaminated nonglass items? - CORRECT ANSWER-
Biohazard bin
Where do you dispose of slides, glass pipets, broken glass, and other sharps? -
CORRECT ANSWER-Glass/ broken sharps bin
What do you do with non-disposable glassware after you are finished using them?
- CORRECT ANSWER-Place all glass vials, glass test tubes, glass slants, and glass
flasks into the dirty dish cart. Remove all tape from glassware. For solid media,
remove the caps from the slants/ tubes and place face down in the baskets. For
liquid media, all tubes or vials should remain capped and placed face up in
baskets. Empty/ contaminated phenol red bottles may be placed in the cart with
labels removed. empty tubes for sterile pipets and wooden sticks may also be
placed face down in the baskets with caps removed. Flasks should be placed in
one of the plastic bins on the lower shelves of the cart.
What is a defined media? - CORRECT ANSWER-One in which all components
have been characterized and are known.
Correct Detailed Answers Already Graded A+
Name the media used for minimal media test? Why is it called a defined media? -
CORRECT ANSWER-The media used for the media test (succinate-NH₃ broth)
contains sodium succinate as the carbon source and ammonium chloride as the
nitrogen source. It is called a defined media because we know exactly what is in it;
there are no materials or substances of unknown composition. It is not a rich
media.
What is the purpose of the minimal media test (what is it trying to find out about
your isolate)? Name one bacteria that cannot grow in minimal media? -
CORRECT ANSWER-The purpose of the minimal media test is to see if the
bacteria are able to grow without a source of enrichment. If the media is cloudy
after inoculation, the isolate does not require sugars and a complex medium for
growth.
One bacteria that cannot grow in minimal media is Streptococcus.
Why do we use King's agar B and not S1 for the Fluorescent Pigment Test? -
CORRECT ANSWER-We use King's Agar B plate instead of the S1 plate because
unlike S1, it is not selective and lacks compounds that inhibit the growth of
, certain types of organisms. It additionally contains phosphorus and magnesium,
which has been demonstrated to increase production of the fluorescent pigment
pyoverdin.
Where do you dispose of plastic Petri dishes, swabs, contaminated oxidase paper,
wooden sticks, and other contaminated nonglass items? - CORRECT ANSWER-
Biohazard bin
Where do you dispose of slides, glass pipets, broken glass, and other sharps? -
CORRECT ANSWER-Glass/ broken sharps bin
What do you do with non-disposable glassware after you are finished using them?
- CORRECT ANSWER-Place all glass vials, glass test tubes, glass slants, and glass
flasks into the dirty dish cart. Remove all tape from glassware. For solid media,
remove the caps from the slants/ tubes and place face down in the baskets. For
liquid media, all tubes or vials should remain capped and placed face up in
baskets. Empty/ contaminated phenol red bottles may be placed in the cart with
labels removed. empty tubes for sterile pipets and wooden sticks may also be
placed face down in the baskets with caps removed. Flasks should be placed in
one of the plastic bins on the lower shelves of the cart.
What is a defined media? - CORRECT ANSWER-One in which all components
have been characterized and are known.
