BIO SCI M118L (MICROBIO LAB) QUIZ 1 LATEST
2026 QUESTIONS and CORRECT DETAILED
ANSWERS ALREADY GRADED A+
Where do you dispose contaminated items? - CORRECT ANSWER-biohazard
containers
Where do you dispose broken glass? - CORRECT ANSWER-disposable glass
containers
Where do you place non-disposable items? - CORRECT ANSWER-dirty dish
carts
What sources do bacteria require to survive? - CORRECT ANSWER-carbon and
nitrogen (possibly amino acids, vitamins, and other growth factors)
Name the two classes of microorganisms (classification based on oxygen
consumption) that utilize oxygen to produce chemical energy (ATP) through the
electron transport chain. - CORRECT ANSWER-strict aerobes and facultative
anaerobes
, Why do we use King's agar B and not S1 for the Fluorescent Pigment Test? -
CORRECT ANSWER-Pigment production on both the S1 plate and the King's
agar B plate will produce a yellow-green color. S1 media, however, contains a
detergent that interferes with the cell membrane of gram positive bacteria,
preventing their growth, and an antibiotic that kills facultative anaerobic gram
negative bacteria. King's agar B is not selective and, therefore, can be used on
more types of bacteria.
King's agar B contains phosphorus and magnesium which is known to increase
production of the fluorescent pigment. King's agar B lacks compounds that inhibit
the growth of certain types of organisms
Why do you never tighten the lids of slants? - CORRECT ANSWER-strict
aerobes wouldn't be able to grow
defined media - CORRECT ANSWER-all components have been characterized
and are known
complex media - CORRECT ANSWER-contains one or more ingredients of
unknown composition
peptones - CORRECT ANSWER-unknown components; nutrient rich extracts
from milk, yeast, beef, etc.
2026 QUESTIONS and CORRECT DETAILED
ANSWERS ALREADY GRADED A+
Where do you dispose contaminated items? - CORRECT ANSWER-biohazard
containers
Where do you dispose broken glass? - CORRECT ANSWER-disposable glass
containers
Where do you place non-disposable items? - CORRECT ANSWER-dirty dish
carts
What sources do bacteria require to survive? - CORRECT ANSWER-carbon and
nitrogen (possibly amino acids, vitamins, and other growth factors)
Name the two classes of microorganisms (classification based on oxygen
consumption) that utilize oxygen to produce chemical energy (ATP) through the
electron transport chain. - CORRECT ANSWER-strict aerobes and facultative
anaerobes
, Why do we use King's agar B and not S1 for the Fluorescent Pigment Test? -
CORRECT ANSWER-Pigment production on both the S1 plate and the King's
agar B plate will produce a yellow-green color. S1 media, however, contains a
detergent that interferes with the cell membrane of gram positive bacteria,
preventing their growth, and an antibiotic that kills facultative anaerobic gram
negative bacteria. King's agar B is not selective and, therefore, can be used on
more types of bacteria.
King's agar B contains phosphorus and magnesium which is known to increase
production of the fluorescent pigment. King's agar B lacks compounds that inhibit
the growth of certain types of organisms
Why do you never tighten the lids of slants? - CORRECT ANSWER-strict
aerobes wouldn't be able to grow
defined media - CORRECT ANSWER-all components have been characterized
and are known
complex media - CORRECT ANSWER-contains one or more ingredients of
unknown composition
peptones - CORRECT ANSWER-unknown components; nutrient rich extracts
from milk, yeast, beef, etc.
