PCB 3134 Final Exam Questions &
Answers (Grade A+)
The sequence of the template DNA strand is 5'-CTGA-3'. What is the
sequence of the mRNA? -
correct answer ✅5'-UCAG-3'
Coding Strand -
correct answer ✅the DNA strand that has the SAME nucleotide
order as the mRNA from 5' to 3'
Template Strand -
correct answer ✅the actual strand that is used as the template for
RNA synthesis (non-coding) and is complementary to the mRNA
Four steps of Transcription -
correct answer ✅1. Binding
2. Initiation
3. Elongation
4. Termination
Pol I makes: -
correct answer ✅most rRNAs - promoter upstream
,PCB 3134 Final Exam Questions &
Answers (Grade A+)
Pol II makes: -
correct answer ✅mRNA, snRNA and niRNA - promoter upstream
Pol III makes: -
correct answer ✅tRNA and one type of rRNA - promoter is within
the transcription unit
TFIID -
correct answer ✅TATA box binding
TFIIH -
correct answer ✅Helicase- melts promoter region
Kinase-phosphorylates Pol II
Transcription by pol I is terminated by: -
correct answer ✅a protein that recognizes an 18-nucleotide signal
in the growing RNA chain
Transcription of Pol III is terminated by: -
correct answer ✅termination signals include short run of Us and
no protein facts are required
, PCB 3134 Final Exam Questions &
Answers (Grade A+)
Transcription of Pol II os terminated by: -
correct answer ✅cleavage at a specific site before transcription
ceases (10-35 nucleotides downstream)
Pre-mRNA -
correct answer ✅crude produce of Pol II transcription, needs
further processing to be mRNA (poly A tail, 5' cap, splicing)
Polyadenylation -
correct answer ✅addition of a poly A tail to the 3' end of mRNA
strand
5' capping -
correct answer ✅addition of a 5' cap to the 5' end. Note the 5'-5'
linkage. Adds stability and ribosome position
Splicing -
correct answer ✅removal of introns and connection of the exons
Alternative Splicing -
correct answer ✅use of alternative axons generates mRNAs that
Answers (Grade A+)
The sequence of the template DNA strand is 5'-CTGA-3'. What is the
sequence of the mRNA? -
correct answer ✅5'-UCAG-3'
Coding Strand -
correct answer ✅the DNA strand that has the SAME nucleotide
order as the mRNA from 5' to 3'
Template Strand -
correct answer ✅the actual strand that is used as the template for
RNA synthesis (non-coding) and is complementary to the mRNA
Four steps of Transcription -
correct answer ✅1. Binding
2. Initiation
3. Elongation
4. Termination
Pol I makes: -
correct answer ✅most rRNAs - promoter upstream
,PCB 3134 Final Exam Questions &
Answers (Grade A+)
Pol II makes: -
correct answer ✅mRNA, snRNA and niRNA - promoter upstream
Pol III makes: -
correct answer ✅tRNA and one type of rRNA - promoter is within
the transcription unit
TFIID -
correct answer ✅TATA box binding
TFIIH -
correct answer ✅Helicase- melts promoter region
Kinase-phosphorylates Pol II
Transcription by pol I is terminated by: -
correct answer ✅a protein that recognizes an 18-nucleotide signal
in the growing RNA chain
Transcription of Pol III is terminated by: -
correct answer ✅termination signals include short run of Us and
no protein facts are required
, PCB 3134 Final Exam Questions &
Answers (Grade A+)
Transcription of Pol II os terminated by: -
correct answer ✅cleavage at a specific site before transcription
ceases (10-35 nucleotides downstream)
Pre-mRNA -
correct answer ✅crude produce of Pol II transcription, needs
further processing to be mRNA (poly A tail, 5' cap, splicing)
Polyadenylation -
correct answer ✅addition of a poly A tail to the 3' end of mRNA
strand
5' capping -
correct answer ✅addition of a 5' cap to the 5' end. Note the 5'-5'
linkage. Adds stability and ribosome position
Splicing -
correct answer ✅removal of introns and connection of the exons
Alternative Splicing -
correct answer ✅use of alternative axons generates mRNAs that
