ASCP BOARD CERTIFICATION MLT EXAM
NEWEST 2026 ACTUAL EXAM TEST
BANK| ASCP MLT BOARD EXAM PREP
WITH COMPLETE 300 REAL EXAM
QUESTIONS AND CORRECT VERIFIED
ANSWERS/ ALREADY GRADED A+
(MOST RECENT!!)
1. In the heterozygous state of HbS, sickle cell trait, which of the following
hemoglobins appear in combination with HbS?
Answer: HbA
2. What is the most likely identity of an acid-fast bacilli that showed buff-
colored, dry, heaped colonies; niacin, nitrate, and urease positive; took 23
days at 35°C to grow?
Answer: Mycobacterium tuberculosis
3. What enzyme can help with the diagnosis of muscular dystrophy?
Answer: CPK (Creatine Phosphokinase)
4. The process of pipetting 1.0 mL of plasma or serum into a tube
containing 1.0 mL of saline, mixing the contents, and then repeating the
same procedure into several additional tubes also containing 1.0 mL of
saline is referred to as:
, Answer: Two-fold serial dilution
5. What would you expect the serum iron (SI) and total iron-binding
capacity (TIBC) to be in a person with hereditary hemochromatosis (HH)?
Answer: Increased SI and decreased TIBC
6. A patient who has a primarily vegetarian diet will most likely have:
Answer: An alkaline urine pH
7. The reticulocyte count is used to assess which of the following?
Answer: RBC production by the bone marrow
8. The most common Rh haplotype among Whites is:
Answer: DCe
9. The tumor marker associated with ovarian cancer is:
Answer: CA-125
10. What is the required storage temperature for thawed cryoprecipitate?
Answer: 20°C to 24°C (room temperature)【0†0】
11. Which of the following is the most suitable specimen for the isolation of
Bordetella pertussis?
Answer: Nasopharyngeal swab
12. Potassium, calcium, and total protein are ______ in capillary specimens,
where glucose is typically ______ than in venous blood.
Answer: higher; higher
, 13. Which molecular method uses fluorescent probes to evaluate genes
and/or DNA sequences on chromosomes?
Answer: FISH (Fluorescence In Situ Hybridization)
14. Oliguria is usually correlated with:
Answer: Acute glomerulonephritis
15. Which test confirms the presence of Bence-Jones proteinuria?
Answer: Immunoelectrophoresis
16. What absorption technique removes cold (IgM) antibodies, particularly
anti-I specificities?
Answer: Rabbit erythrocyte stroma
17. A 300 µg dose of RhIg can suppress immunization of how many mL of
D+ blood?
Answer: 30 mL
18. The radioactive method used to measure red cell survival uses what
isotope?
Answer: Cr51 (Chromium 51)
19. Patients with Cushing's disease typically exhibit what?
Answer: Serum cortisol overproduction
20. Donor and recipient blood samples must be kept for how long after
transfusion?
Answer: 7 days
, 21. How often should fire drills be conducted in the laboratory?
Answer: Yearly
22. Contact dermatitis is mediated by:
Answer: T-Lymphocytes
23. What method is used to confirm the presence of Bence-Jones protein in
the urine?
Answer: Urine protein electrophoresis or immunoelectrophoresis
24. An India ink preparation is used on CSF to identify:
Answer: Cryptococcus neoformans
25. Which antibodies react most strongly at 4°C?
Answer: Anti-A, Anti-P1, Anti-Leᵇ, and Anti-M
26. Which of the following may be associated with acidic urinary pH?
Answer: High urine glucose
27. Parasites that may be found in urinary sediment include all of the
following EXCEPT:
Answer: Enterobius vermicularis
28. The urease reaction seen in the Christensen's urea agar slant on the far
right indicates:
Answer: Weak activity
NEWEST 2026 ACTUAL EXAM TEST
BANK| ASCP MLT BOARD EXAM PREP
WITH COMPLETE 300 REAL EXAM
QUESTIONS AND CORRECT VERIFIED
ANSWERS/ ALREADY GRADED A+
(MOST RECENT!!)
1. In the heterozygous state of HbS, sickle cell trait, which of the following
hemoglobins appear in combination with HbS?
Answer: HbA
2. What is the most likely identity of an acid-fast bacilli that showed buff-
colored, dry, heaped colonies; niacin, nitrate, and urease positive; took 23
days at 35°C to grow?
Answer: Mycobacterium tuberculosis
3. What enzyme can help with the diagnosis of muscular dystrophy?
Answer: CPK (Creatine Phosphokinase)
4. The process of pipetting 1.0 mL of plasma or serum into a tube
containing 1.0 mL of saline, mixing the contents, and then repeating the
same procedure into several additional tubes also containing 1.0 mL of
saline is referred to as:
, Answer: Two-fold serial dilution
5. What would you expect the serum iron (SI) and total iron-binding
capacity (TIBC) to be in a person with hereditary hemochromatosis (HH)?
Answer: Increased SI and decreased TIBC
6. A patient who has a primarily vegetarian diet will most likely have:
Answer: An alkaline urine pH
7. The reticulocyte count is used to assess which of the following?
Answer: RBC production by the bone marrow
8. The most common Rh haplotype among Whites is:
Answer: DCe
9. The tumor marker associated with ovarian cancer is:
Answer: CA-125
10. What is the required storage temperature for thawed cryoprecipitate?
Answer: 20°C to 24°C (room temperature)【0†0】
11. Which of the following is the most suitable specimen for the isolation of
Bordetella pertussis?
Answer: Nasopharyngeal swab
12. Potassium, calcium, and total protein are ______ in capillary specimens,
where glucose is typically ______ than in venous blood.
Answer: higher; higher
, 13. Which molecular method uses fluorescent probes to evaluate genes
and/or DNA sequences on chromosomes?
Answer: FISH (Fluorescence In Situ Hybridization)
14. Oliguria is usually correlated with:
Answer: Acute glomerulonephritis
15. Which test confirms the presence of Bence-Jones proteinuria?
Answer: Immunoelectrophoresis
16. What absorption technique removes cold (IgM) antibodies, particularly
anti-I specificities?
Answer: Rabbit erythrocyte stroma
17. A 300 µg dose of RhIg can suppress immunization of how many mL of
D+ blood?
Answer: 30 mL
18. The radioactive method used to measure red cell survival uses what
isotope?
Answer: Cr51 (Chromium 51)
19. Patients with Cushing's disease typically exhibit what?
Answer: Serum cortisol overproduction
20. Donor and recipient blood samples must be kept for how long after
transfusion?
Answer: 7 days
, 21. How often should fire drills be conducted in the laboratory?
Answer: Yearly
22. Contact dermatitis is mediated by:
Answer: T-Lymphocytes
23. What method is used to confirm the presence of Bence-Jones protein in
the urine?
Answer: Urine protein electrophoresis or immunoelectrophoresis
24. An India ink preparation is used on CSF to identify:
Answer: Cryptococcus neoformans
25. Which antibodies react most strongly at 4°C?
Answer: Anti-A, Anti-P1, Anti-Leᵇ, and Anti-M
26. Which of the following may be associated with acidic urinary pH?
Answer: High urine glucose
27. Parasites that may be found in urinary sediment include all of the
following EXCEPT:
Answer: Enterobius vermicularis
28. The urease reaction seen in the Christensen's urea agar slant on the far
right indicates:
Answer: Weak activity
