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Summary Lab test 1 PTC PCR Exam Q & As (Verified Answers) 2026

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Lab test 1 PTC PCR Exam Q & As (Verified Answers) 2026

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Lab test 1 PTC PCR Exam Q & As
(Verified Answers) 2026

5 basic tastes - CORRECT ANSWERS sweet, sour, bitter, salty, umami



taste perception steps - CORRECT ANSWERS a taste molecule binds to a specific receptor
on the surface of a taste cell



the taste cell generates a nervous impulse, which is interpreted by the brain



stimulation of sweet cells generates a perception of _____ - CORRECT ANSWERS
sweetness in the brain



DuPont observation in the 1930s - CORRECT ANSWERS Arthur Fox and CR Noller

Noller tasted the bitter PTC dust



studies done by _____ _______ showed that the inability to taste PTC is a _____ trait that varies in
human population - CORRECT ANSWERS albert blackeslee

recessive



there are ______ genes for different bitter taste receptors in mammals - CORRECT ANSWERS
30



the gene for the PTC taste receptor - CORRECT ANSWERS TAS2R38



TAS2R38 - CORRECT ANSWERS 3 nucleotides that vary

, each variable is termed a SNP (single nucleotide polymorphism)

a haplotype correlated most



haplotype - CORRECT ANSWERS a combination of 3 SNP's



in the first two lab sessions, a sample of human cells is obtained by... - CORRECT ANSWERS
saline mouthwash

section 1 step 1



DNA is extracted by boiling with _____ which _________________ - CORRECT ANSWERS
Chelex resin

binds contaminating metal ions

section 1 step 2



________ is used to amplify a short region of the TAs2R38 gene - CORRECT ANSWERS PCR

section 1 step 3



the amplified PCR product is digested with the restriction enzyme HaeIII, whose recognition sequence
includes one of the SNPs. One allele is cut by the enzyme, and one is not--producing a restrictin
fragment length polymorphism (RFLP) that can be separated on a 2% agarose gel. Each student scores
his or her genotype, predicts their tasting abilty, and then tastes PTC paper - CORRECT ANSWERS
second session



1 microliter - CORRECT ANSWERS 1 millionth of a Liter is uL



Micropipettors sizes



P20

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