MCB 3020C Microbiology Lab UCF EXAM | QUESTIONS AND ANSWERS | 2026 UPDATE | WITH COMPLETE SOLUTIONS 100% CORRECT

MCB 3020C Microbiology Lab UCF
Exam 2 Newest With Questions And
Answers 2026/2027 Graded A


Most dyes have two different components: - ANSWER>Solvent and chromogen




-Chromogen: provides the color and charge to the dyes used for staining




Cationic dyes - ANSWER>BASIC dyes, positively charged chromogen:


.


-methylene blue


-crystal violet




Anionic dyes - ANSWER>ACIDIC dyes, negatively charged chromogin:


.


-acid fuchsin


-Congo red

, -Nigrosin




Smearing from broth culture - ANSWER>1. sterilize and cool loop


2. remove the cap of the tube with pinky finger


3. sterilize mouth of tube


4. collect sample


5. sterilize and recap tube


6. make smear, spreading only 1 loopful of culture and spread until maximum thinness


7. sterilize loop


8. Heat fix




Smearing from solid culture (Plate or slant) - ANSWER>1. Add 1 loopful of water


2. remove the cap of the tube with pinky finger


3. sterilize mouth of tube


4. collect sample-just touch the loop to bacteria growth on the plate or slant, mix well in drop of water to suspend


5. sterilize and recap tube


6. make smear