MCB 3020C Microbiology Lab UCF
Exam 4 Newest With Questions And
Answers 2026/2027 Graded A
Inhibitor - ANSWER>prevent the growth of certain bacteria, which allows you to select for other species that can
grow in the presence of these inhibitors
Indicators - ANSWER>detect changes in the media based on a process that has occurred
-either dyes of pH indicators
Complex media - ANSWER>composed of raw materials and are usually undefined, meaning the exact chemical
composition is not known
Defined media - ANSWER>Have a known chemical composition and exact amount for each component.
-designed for isolation, selection or differentiation of specific bacteria
, Phenylethyl Alcohol Agar (PEA) - ANSWER>-Selective Medium, containing phenylethyl alcohol
-Inhibitor: inhibits growth against Gram(-) bacteria
-Selects for Gram(+) bacteria
-ex. S. aureus
Blood Agar (BA) - ANSWER>-Differential medium composed of a tryptocase soy agar base with 5% sheeps blood
-Differentiates bacteria based on hemolysis patterns
Beta: complete hemolysis of RBC, clearing around bacterial colonies
Alpha: Incomplete (Partial) hemolysis of RBC, discoloration around bacterial colonies as either green or brown
Gamma: No hemolysis of RBC, no change in bacterial colonies
Exam 4 Newest With Questions And
Answers 2026/2027 Graded A
Inhibitor - ANSWER>prevent the growth of certain bacteria, which allows you to select for other species that can
grow in the presence of these inhibitors
Indicators - ANSWER>detect changes in the media based on a process that has occurred
-either dyes of pH indicators
Complex media - ANSWER>composed of raw materials and are usually undefined, meaning the exact chemical
composition is not known
Defined media - ANSWER>Have a known chemical composition and exact amount for each component.
-designed for isolation, selection or differentiation of specific bacteria
, Phenylethyl Alcohol Agar (PEA) - ANSWER>-Selective Medium, containing phenylethyl alcohol
-Inhibitor: inhibits growth against Gram(-) bacteria
-Selects for Gram(+) bacteria
-ex. S. aureus
Blood Agar (BA) - ANSWER>-Differential medium composed of a tryptocase soy agar base with 5% sheeps blood
-Differentiates bacteria based on hemolysis patterns
Beta: complete hemolysis of RBC, clearing around bacterial colonies
Alpha: Incomplete (Partial) hemolysis of RBC, discoloration around bacterial colonies as either green or brown
Gamma: No hemolysis of RBC, no change in bacterial colonies
