MCB 3023: UF- Exam 2,3 And 4 Test
Questions with Accurate Solutions
2026-2027 Updated.
What are micro RNAs?
a. tRNAs
b. small regulatory RNAs
c. rRNAs
d. small mRNAs
e. mRNAs that encode for regulatory proteins - Answer b. small regulatory RNAs
Replication of the bacterial chromosome begins at how many locations?
a. One
b. Three
c. Four
d. Two
e. Five - Answer a. one
Which of these best differentiates DNA and RNA?
a. RNA contains ribose and DNA contains deoxyribose
b. RNA is single-stranded and DNA is double stranded
,c. All statements are true
d. RNA contains the base uracil in place of thymine, which is in DNA - Answer c. All
statements are true
Which enzyme is responsible for adding the final bases to the end of a chromosome during DNA
replication in eukaryal cells?
a. DNA polδ
b. DNA primase B
c. topoisomerase
d. DNA polα
e. Telomerase - Answer e. Telomerase
Term for cells that can naturally take up extracellular DNA
a. primed
b. susceptible
c. conjugant
d. porous
e. competent - Answer e. competent
Suppose a mutant strain of Escherichia coli K12 contains a mutation in the trpE gene and is no
longer able to make tryptophan. What is this mutant called?
a. A phenotroph
b. An auxotroph
,c. A lithotroph
d. A prototroph
e. An autotroph - Answer b. An auxotroph
Which term applies to the transfer of genes from mother to daughter cell?
a. horizontal gene transfer
b. meiosis
c. transduction
d. vertical gene transfer
e. lateral gene transfer - Answer d. vertical gene transfer
The process of molecular cloning was mainly brought about through the discovery of these
a. bacteriophages
b. restriction enzymes
c. transformations
d. conjugation events
e. transposons - Answer b. restriction enzymes
Bacterial mutants have been very useful for studying and understanding:
a. capsule production
, b. carbon metabolism
c. antibiotic resistance
d. all of these choices
e. biosynthetic pathways - Answer d. all of these choices are correct
What is metagenomics?
a. A culture-dependent method that may be used to determine protein sequences
b. A culture -independent method that may be used for the isolation of different organisms
c. A culture-dependent method that may be used to study groups of microbes in an ecosystem
d. A culture-independent method that may be used to document microbial community pools of
genetic material
e. A culture-dependent method that may be used to find new enzymes - Answer d. A culture-
independent method that may be used to document microbial community pools of genetic
material
The sequencing method which uses detection of pyrophosphate liberated when nucleotides are
added to a growing DNA chain is called:
a. Sanger dideoxy sequencing
b. Ion torrent sequencing
c. Pyrosequencing
d. PacBio sequencing
e. Plus-minus sequencing - Answer c. Pyrosequencing
Questions with Accurate Solutions
2026-2027 Updated.
What are micro RNAs?
a. tRNAs
b. small regulatory RNAs
c. rRNAs
d. small mRNAs
e. mRNAs that encode for regulatory proteins - Answer b. small regulatory RNAs
Replication of the bacterial chromosome begins at how many locations?
a. One
b. Three
c. Four
d. Two
e. Five - Answer a. one
Which of these best differentiates DNA and RNA?
a. RNA contains ribose and DNA contains deoxyribose
b. RNA is single-stranded and DNA is double stranded
,c. All statements are true
d. RNA contains the base uracil in place of thymine, which is in DNA - Answer c. All
statements are true
Which enzyme is responsible for adding the final bases to the end of a chromosome during DNA
replication in eukaryal cells?
a. DNA polδ
b. DNA primase B
c. topoisomerase
d. DNA polα
e. Telomerase - Answer e. Telomerase
Term for cells that can naturally take up extracellular DNA
a. primed
b. susceptible
c. conjugant
d. porous
e. competent - Answer e. competent
Suppose a mutant strain of Escherichia coli K12 contains a mutation in the trpE gene and is no
longer able to make tryptophan. What is this mutant called?
a. A phenotroph
b. An auxotroph
,c. A lithotroph
d. A prototroph
e. An autotroph - Answer b. An auxotroph
Which term applies to the transfer of genes from mother to daughter cell?
a. horizontal gene transfer
b. meiosis
c. transduction
d. vertical gene transfer
e. lateral gene transfer - Answer d. vertical gene transfer
The process of molecular cloning was mainly brought about through the discovery of these
a. bacteriophages
b. restriction enzymes
c. transformations
d. conjugation events
e. transposons - Answer b. restriction enzymes
Bacterial mutants have been very useful for studying and understanding:
a. capsule production
, b. carbon metabolism
c. antibiotic resistance
d. all of these choices
e. biosynthetic pathways - Answer d. all of these choices are correct
What is metagenomics?
a. A culture-dependent method that may be used to determine protein sequences
b. A culture -independent method that may be used for the isolation of different organisms
c. A culture-dependent method that may be used to study groups of microbes in an ecosystem
d. A culture-independent method that may be used to document microbial community pools of
genetic material
e. A culture-dependent method that may be used to find new enzymes - Answer d. A culture-
independent method that may be used to document microbial community pools of genetic
material
The sequencing method which uses detection of pyrophosphate liberated when nucleotides are
added to a growing DNA chain is called:
a. Sanger dideoxy sequencing
b. Ion torrent sequencing
c. Pyrosequencing
d. PacBio sequencing
e. Plus-minus sequencing - Answer c. Pyrosequencing
