2026 QUESTIONS WITH CORRECT
ANSWERS GRADED A+
◍ Name the 3rd generation cephalosporins.
Answer: •Ceftizoxime•Ceftriaxone•Ceftazidine•Cefotaxime•Cefoperozone
◍ Antibiotics which inhibit protein synthesis include tetracyclines,
erythromycin, azithromycin, clindamycin, __________, and
_____________..
Answer: Aminoglycosides & chloramphenicol
◍ Acridine Orange Stain.
Answer: Stains nucleic acids of living or dead cells.Prepare slide and fix
with methanol.Flood with stain, rinse and examine under 40X.Bacteria /
Yeast will fluoresce orange.
◍ Porphyromonas gingivalis.
Answer: -Anaerobic GNR-Brown/black colonies on LKV-Fluoresces red
under UV light-Resistant to Kana and Col-Susceptible to Vanco and Bile
◍ Bacteria can become resistance due to low affinity target sites. What is an
example of an altered target site?.
Answer: Altered penicillin-binding sites have caused resistance in
pneumococcus
◍ Beta-Lactamases.
Answer: MOs produce enzymes that cleave the B-Lactam
ring.PenicillinaseCephaloporinaseExtended Spectrum Beta Lactamase
(ESBL)
◍ Oxidizer.
, Answer: Metabolizes carbohydrates in ari
◍ How does the ESP/Trek blood culture machine detect bacterial growth?.
Answer: ESP/Trek machine detects pressure changes
◍ Throat Pathogens.
Answer: Group A, C, G StrepArcanobacterium
haemolyticumGonococciViruses
◍ Monobactams.
Answer: Beta Lactam GroupAztreonam
◍ Special susceptibility testing for enterics (ESBL).
Answer: ESBL-Streak MH agar for confluent growth-Add disks to be tested
-2, 3rd gen cephalosporin -2 of the same disks + clavulanic acid-Incubate
overnight-Measure zone of the antibiotics alone-Measure zone of the
antibiotics + CLA ***-If there is a difference of >5mm, it is ESBL +-If the
MO being tested is ESBL +, it is resistant to penicillin's, cephalosporin,
aztreonam
◍-
V. cholerae-
V. alinolyticus-yellow.
Answer: Which Vibrio species are sucrose fermentors and what color is the
reaction?
◍ Which organisms are intrinsically resistant to clindamycin?.
Answer: Gram negative MOs
◍ How should normally sterile body fluids be stored if they cannot be set-up
immediately on arrival to the laboratory?.
Answer: They should be stored at room temperature.
◍ Oxacillin is in the same class of drugs as methicillin and nafcillin. If a
staphylococci isolate is resistant to oxacillin, can the patient be treated with
methicillin or nafcillin?.
Answer: Resistance to oxacillin means resistance to all the drugs in the class
, ◍ If a patient was diagnosed with an anaerobic infection, which antibiotic
would most likely be prescribed?.
Answer: metronidazole
◍ Cardiobacterium hominis.
Answer: Rosette morphology on the gram stain
◍ Which drugs are included in the carboxypenicillins?.
Answer: Carbenicillin & ticarcillin
◍ What is the most common pathogen that causes otitis externa?.
Answer: PSAR
◍ Which drugs cannot be reported if Staphylococci is resistant to cefoxitin?.
Answer: The beta-lactam drugs cannot be reported EVEN if they appear
sensitivity
◍ What plates do we subculture genital specimens?What temperature and
atmosphere is needed?.
Answer: -BAP, MTM-37°C in CO2-Gram stain genital specimens and look
for the following; clue cells, gram negative diplococci, yeast
◍ Special media used for lower respiratory tract?.
Answer: BCYE, BCSA/OFPBL, Regan Lowe
◍ Antibiotic.
Answer: Substances produced by MO which kills or inhibits other MO's
◍ What is the MBC results from the image shown below:.
Answer: 8 micrograms/mL
◍ RNA synthesis inhibitors.
Answer: Rifampin
◍ Epiglottis Culture.
Answer: BAPCHOCMACIncubate at 35C in CO2
◍ Campylobacter spp (thermophilic).
Answer: -Campylobacter coli - hippurate =-Campylobacter jejuni -