UNCW BIO 201 2 CERTIFICATION
SCRIPT 2026 QUESTIONS WITH
SOLUTIONS GRADED A+
◍ pH Meters.
Answer: Measure the acidity of solutions.
◍ Amphipathic Macromolecules.
Answer: Both hydrophilic and hyrdrophobic
◍ Acidity.
Answer: The concentration of hydrogen ions (H+) in a solution.
◍ Examples of Amphipathic Macromolecules.
Answer: 1. Phospholipids 2. Proteins in a membrane
◍ pH is Logarithmic.
Answer: pH=-log[H+]
◍ Buffer.
Answer: A solution which resists changes in pH when acids or bases are
added to them. Most buffers contain approximately equal amounts of weak
acid and its related base.
◍ Spectrophotometer.
Answer: Measure light absorbance by substances, called pigments, in a
solution.
◍ Cuvette.
Answer: What a solution is put in to go into the Spectrophotometer.
◍ Absorbance.
Answer: log(Initial Intensity of light/Light Intensity at Sample)
,◍ Beer-Lambert Law.
Answer: A=abc. (A) is absorbance. (a) is the absorbance coefficient which is
constant for the particular substance. (b) is the path length or the distance
light must travel through the sample. (c) is the concentration of the pigment.
◍ Membrane Characteristics.
Answer: A. Membranes form spontaneouslyB. Membrane structure is tightly
controlled by cells.We call the resulting structure a fluid mosaic.1. 2
semifluid leaflets of lipids2. 2 classes of proteins with numerous functions3.
Carbohydrates face the outside of the cellC. Membrane "mosaic" structure
enablesmembrane function, and membrane have alot of functions to
perform!
◍ An Ideal Pigment.
Answer: Its absorbance should double when its concentration is doubled.
This is because absorbance is linearly proportional to its concentration.
◍ Hydrophobic Interactions.
Answer: cage-like structure of waterforms around hydrophobe
◍ Standard Curve.
Answer: A plot of the absorbance at a single wavelength obtained from a
number of solutions of a known concentration.
◍ What was the purpose of the serial dilution of "smurf blood" and plotting the
values on a graph?.
Answer: The purpose was to see how the change in concentration of a
solution effects the absorbance of the solution.
◍ What did you observe in general of absorbance values as the solution
became more diluted?.
Answer: As the solution increases in concentration the Absorbance values
also increase. This means that as the solution becomes more diluted, the
absorbance values decrease.
◍ When an acid is added to an unbuffered solution, what will happen to pH
and [H+]?.
, Answer: pH will decrease as [H+] increases.
◍ Step 1 of membrane fluidity.
Answer: Step 1. Hydrophobic exclusion pushes fatty acids tails of
phospholipids together
◍ What are the 4 classes of biological macromolecules?.
Answer: Carbohydrates, Lipids, Proteins, and Nucleic Acids.
◍ What is the difference between a qualitative and a quantitative test? Be
specific..
Answer: Qualitative is taking physical characteristics into account like color
or smell whereas quantitative is numerical values that can be gathered from
the data.
◍ Step 2 of membrane fluidity.
Answer: H-bonds andhydration spheres formbetween water &
thehydrophilic heads.
◍ Step 3 of membrane fluidity.
Answer: van der Waalsinteractions among thehydrophobic portions
ofmolecules determinesmembrane fluidity.
◍ Relationship between fluidity and Van der Waals forces.
Answer: More van der Waalsequals less fluidity.
◍ Leaflet.
Answer: one half(layer) of thephospholipid bilayer
◍ Organic Molecules.
Answer: Composed primarily of carbon, hydrogen, and oxygen.
◍ Most Common Organic Compounds.
Answer: Proteins, Carbohydrates, Lipids, and Nucleic Acids.
◍ Van der Waals purpose in the cell membrane.
Answer: Hydrophobic portionsof membrane (tails of
phospholipids)molecules interact witheach other by van derWaals forces.
Thesetemporary bonds makethe membrane "fluid".
SCRIPT 2026 QUESTIONS WITH
SOLUTIONS GRADED A+
◍ pH Meters.
Answer: Measure the acidity of solutions.
◍ Amphipathic Macromolecules.
Answer: Both hydrophilic and hyrdrophobic
◍ Acidity.
Answer: The concentration of hydrogen ions (H+) in a solution.
◍ Examples of Amphipathic Macromolecules.
Answer: 1. Phospholipids 2. Proteins in a membrane
◍ pH is Logarithmic.
Answer: pH=-log[H+]
◍ Buffer.
Answer: A solution which resists changes in pH when acids or bases are
added to them. Most buffers contain approximately equal amounts of weak
acid and its related base.
◍ Spectrophotometer.
Answer: Measure light absorbance by substances, called pigments, in a
solution.
◍ Cuvette.
Answer: What a solution is put in to go into the Spectrophotometer.
◍ Absorbance.
Answer: log(Initial Intensity of light/Light Intensity at Sample)
,◍ Beer-Lambert Law.
Answer: A=abc. (A) is absorbance. (a) is the absorbance coefficient which is
constant for the particular substance. (b) is the path length or the distance
light must travel through the sample. (c) is the concentration of the pigment.
◍ Membrane Characteristics.
Answer: A. Membranes form spontaneouslyB. Membrane structure is tightly
controlled by cells.We call the resulting structure a fluid mosaic.1. 2
semifluid leaflets of lipids2. 2 classes of proteins with numerous functions3.
Carbohydrates face the outside of the cellC. Membrane "mosaic" structure
enablesmembrane function, and membrane have alot of functions to
perform!
◍ An Ideal Pigment.
Answer: Its absorbance should double when its concentration is doubled.
This is because absorbance is linearly proportional to its concentration.
◍ Hydrophobic Interactions.
Answer: cage-like structure of waterforms around hydrophobe
◍ Standard Curve.
Answer: A plot of the absorbance at a single wavelength obtained from a
number of solutions of a known concentration.
◍ What was the purpose of the serial dilution of "smurf blood" and plotting the
values on a graph?.
Answer: The purpose was to see how the change in concentration of a
solution effects the absorbance of the solution.
◍ What did you observe in general of absorbance values as the solution
became more diluted?.
Answer: As the solution increases in concentration the Absorbance values
also increase. This means that as the solution becomes more diluted, the
absorbance values decrease.
◍ When an acid is added to an unbuffered solution, what will happen to pH
and [H+]?.
, Answer: pH will decrease as [H+] increases.
◍ Step 1 of membrane fluidity.
Answer: Step 1. Hydrophobic exclusion pushes fatty acids tails of
phospholipids together
◍ What are the 4 classes of biological macromolecules?.
Answer: Carbohydrates, Lipids, Proteins, and Nucleic Acids.
◍ What is the difference between a qualitative and a quantitative test? Be
specific..
Answer: Qualitative is taking physical characteristics into account like color
or smell whereas quantitative is numerical values that can be gathered from
the data.
◍ Step 2 of membrane fluidity.
Answer: H-bonds andhydration spheres formbetween water &
thehydrophilic heads.
◍ Step 3 of membrane fluidity.
Answer: van der Waalsinteractions among thehydrophobic portions
ofmolecules determinesmembrane fluidity.
◍ Relationship between fluidity and Van der Waals forces.
Answer: More van der Waalsequals less fluidity.
◍ Leaflet.
Answer: one half(layer) of thephospholipid bilayer
◍ Organic Molecules.
Answer: Composed primarily of carbon, hydrogen, and oxygen.
◍ Most Common Organic Compounds.
Answer: Proteins, Carbohydrates, Lipids, and Nucleic Acids.
◍ Van der Waals purpose in the cell membrane.
Answer: Hydrophobic portionsof membrane (tails of
phospholipids)molecules interact witheach other by van derWaals forces.
Thesetemporary bonds makethe membrane "fluid".
