BIOL 402 Exam 2 || 100% Faultless Solutions.
What is the purpose of the inclusion of a Durham tube in a differential culture media? correct
answers to visualize the production of gas during growth
In the urease assay, a positive result is recorded if the culture media becomes more correct
answers basic
What is considered a positive result in the gelatinase test? correct answers liquefaction of media
Ames test: which plates do you need to examine to determine if your test substance is
mutagenic? correct answers minimal media plate (MM)
Ames test: why can't your test substance [the potential mutagen] contain protein? correct answers
bacteria could obtain histidine from the test substance
Addition of 300 microlitters sample to 2.7 milliliters is a ____-fold dilution. Addition of 30
microliters sample to 2.97 milliliters of diluent would be a ___-fold dilution correct answers 10,
100
Gas-Pack (anaerobe jars): an alternative to the anaerobe lab is a candle jar, in which a candle is
placed in the jar, lit, and the lid closed (to extinguish the flame). Typically, not all of the oxygen
is used. Which organisms would most likely benefit from this environment? correct answers
capnophiles
Where do the energy-producing steps of the carbohydrate fermentation take place? correct
answers inside individual bacterial cells
Are the nitrile gloves we use in lab sterile correct answers no
The original conc. in a sample is 2 x 10^8 CFU/mL. Application of which sample volume would
yield a plate having 200 colonies? correct answers 1 nanoliter
You have inoculated 100 microliters of a sample that had been diluted by a factor of 10^-6, onto
a TSA plate. After incubation, you count 31 colonies on the plate. What is the original cell
density? correct answers 3.1 x 10^8
A colony picked directly from ____ media should never be considered a pure culture correct
answers selective media
The methyl red test is intended to detect organisms that are capable of correct answers
performing mixed acid fermentation of glucose
The tests we have used in this class for differentiation of species have directly examined correct
answers bacterial phenotype (expressed biochemical activities)
What is the purpose of the inclusion of a Durham tube in a differential culture media? correct
answers to visualize the production of gas during growth
In the urease assay, a positive result is recorded if the culture media becomes more correct
answers basic
What is considered a positive result in the gelatinase test? correct answers liquefaction of media
Ames test: which plates do you need to examine to determine if your test substance is
mutagenic? correct answers minimal media plate (MM)
Ames test: why can't your test substance [the potential mutagen] contain protein? correct answers
bacteria could obtain histidine from the test substance
Addition of 300 microlitters sample to 2.7 milliliters is a ____-fold dilution. Addition of 30
microliters sample to 2.97 milliliters of diluent would be a ___-fold dilution correct answers 10,
100
Gas-Pack (anaerobe jars): an alternative to the anaerobe lab is a candle jar, in which a candle is
placed in the jar, lit, and the lid closed (to extinguish the flame). Typically, not all of the oxygen
is used. Which organisms would most likely benefit from this environment? correct answers
capnophiles
Where do the energy-producing steps of the carbohydrate fermentation take place? correct
answers inside individual bacterial cells
Are the nitrile gloves we use in lab sterile correct answers no
The original conc. in a sample is 2 x 10^8 CFU/mL. Application of which sample volume would
yield a plate having 200 colonies? correct answers 1 nanoliter
You have inoculated 100 microliters of a sample that had been diluted by a factor of 10^-6, onto
a TSA plate. After incubation, you count 31 colonies on the plate. What is the original cell
density? correct answers 3.1 x 10^8
A colony picked directly from ____ media should never be considered a pure culture correct
answers selective media
The methyl red test is intended to detect organisms that are capable of correct answers
performing mixed acid fermentation of glucose
The tests we have used in this class for differentiation of species have directly examined correct
answers bacterial phenotype (expressed biochemical activities)
