Life Science: W10 PEQs Test Questions and All Correct Answers.

Life Science: W10 PEQs Test Questions
and All Correct Answers.
Shown below is the start of a coding region within the first exon of a gene. Which sequence
could you use for your sgRNA?



5'-GCTCTTAGATATTCCACGACACAGCATGTCAAGAGAAGTACAGTAATGACGGACTAGTA-3'

3'-CGAGAATCTATAAGGTGCTGTGTCGTACAGTTCTCTTCATGTCATTACTGCCTGATCAT-5'



(a) 3' CGACACAGCATGTCAAGAGA 5'

(b) 5' CGACACAGCATGTCAAGAGA 3'

(c) 3' GCTGTGTCGTACAGTTCTCT 5'

(d) 5' GCTGTGTCGTACAGTTCTCT 3' - Answer (c) 3' GCTGTGTCGTACAGTTCTCT 5'



Shown below is the start of a coding region within the first exon of a gene. How many Cas9 PAM
sequences are present?



5'-GCTCTTAGATATTCCACGACACAGCATGTCAAGAGAAGTACAGTAATGACGGACTAGTA-3'

3'-CGAGAATCTATAAGGTGCTGTGTCGTACAGTTCTCTTCATGTCATTACTGCCTGATCAT-5'



(a) 0

(b) 3

(c) 2

(d) 4

(e) 1 - Answer (c) 2



Shown below is part of a coding region within the first exon of a gene. Where will Cas9 cause a
double stranded DNA break?



(a) B

(b) A

(c) D

(d) C - Answer (b) A