Questions and Guide Answers
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1. True or False Growth media is best suited for distinguishing between two
similar species of bacteria
Answer: False. Growth media is designed to simply support (and not restrict) microbial growth
2. A researcher is asked to determine which of two vials contains e coli and which
contains salmonella. Knowing both gram-negative while only one of them is
capable of fermenting lactose, which type of media would be best suited (multiple
choice)
Answer: Ditterential media distinguishes between two, often related, microbes
3. What are the requirements of a fastidious microbe? (short answer)
Answer: A fastidious microbe is an organism with complex growth requirements such that is absent it will not
grow. Enriched medias thus contain these specific and essential nutrients required for the growth of a particular
, subset of microorganisms.
4. True or False LB agar is classified as a selective, non-differential media
Answer: False
5. What is agar used for in microbiology? (short answer)
Answer: Agar is used to create a solid, smooth surface on which microbes can grow
6. Alpha hemolysis (matching)
Answer: Incomplete hemolytic activity
7. Beta hemolysis (matching)
Answer: Complete hemolytic activity
8. Gamma hemolysis (matching)
Answer: No hemolytic activity
9. Colombia CNA agar is most closely related to which media (multiple choice)-
Answer: CNA agar is similar to VAP as it is also enriched with blood and allows for ditterentiation based on hemolytic patterns
10. True or False Chocolate agar gets its brown color from cocoa to produce
an enriched media
Answer: False
11. A researcher is studying a strain of e coli currently growing on a MacConkey
plate. However, the researcher can't remember if e coli is gram positive or gram