Portage Microbiology Exam #3
Questions and Guide Answers
100% Verified Graded A+
1. What is the total magnification of a sample imaged with a 60x objective and a 10x
eyepiece? (short answer)
Answer: 60 x 10 = 600x magnification
2. True or False A cell that is adherent, flat, and unstained is easily identified using bright
field microscopy
Answer: False. Adherent, flat cells are almost invisible due to the limits on both
resolution and contrast
3. Which of the following could be seen clearly by the unaided eye? (multiple choice)
Answer: Protozoa with diameter of 150um and skin cell with diameter of 1500 um (have to be greater than 100um
4. Label the following unmarked microscope components by matching it with the
components provided
, Answer: 1. Eyepiece
2. Neck
3. Fine adjustment knob
4. Objective
5. Stage
6. Base
5. This type of microscope is best suited for visualizing GFP, RFP and YFP
proteins (given list of microscopes)
Answer: Flourescence
6. This type of microscope can provide detailed images of live cells without staining
(given list of microscopes)
Answer: Phase-Contrast
7. This type of microscope is used to greatly increase the contrast between samples
and background by reflecting light off of the specimen (given list of microscopes)
Answer: Dark Field
8. This type of microscope is capable of capturing images in multiple focal planes
rendering specimen in 3D (given list of microscopes)
Answer: Confocal
9. Identify what type of electron microscope was used to capture the following image and
Questions and Guide Answers
100% Verified Graded A+
1. What is the total magnification of a sample imaged with a 60x objective and a 10x
eyepiece? (short answer)
Answer: 60 x 10 = 600x magnification
2. True or False A cell that is adherent, flat, and unstained is easily identified using bright
field microscopy
Answer: False. Adherent, flat cells are almost invisible due to the limits on both
resolution and contrast
3. Which of the following could be seen clearly by the unaided eye? (multiple choice)
Answer: Protozoa with diameter of 150um and skin cell with diameter of 1500 um (have to be greater than 100um
4. Label the following unmarked microscope components by matching it with the
components provided
, Answer: 1. Eyepiece
2. Neck
3. Fine adjustment knob
4. Objective
5. Stage
6. Base
5. This type of microscope is best suited for visualizing GFP, RFP and YFP
proteins (given list of microscopes)
Answer: Flourescence
6. This type of microscope can provide detailed images of live cells without staining
(given list of microscopes)
Answer: Phase-Contrast
7. This type of microscope is used to greatly increase the contrast between samples
and background by reflecting light off of the specimen (given list of microscopes)
Answer: Dark Field
8. This type of microscope is capable of capturing images in multiple focal planes
rendering specimen in 3D (given list of microscopes)
Answer: Confocal
9. Identify what type of electron microscope was used to capture the following image and
