BCH 2333 Lab 2: Amino acids and Proteins Study Guide with Complete
Solution | 2026 Update
LAB 2: Amino acids and Proteins
Purpose:
, This lab includes two experiments. The goal of the first experiment is to separate
the three components of a mixture of glycine, aspartic acid and lysine by performing an
ion-exchange chromatography. Furthermore, the aim of this experiment is to understand
the order of elution which is based upon the differences in the relative number and
strength of the basic and acidic groups in the amino acids. The goal of the second
experiment is to separate the components of a mixture of MW marker proteins by
performing an electrophoresis on a polyacrylamide gel in the presence of SDS and to also
determine the MW of an unknown protein.
Results and Discussion Ion-exchange chromatography
R1. Glycine, Aspartic acid, and Lysine at pH 3.9
Figure 1. Structures, pKa and PI of Glycine, Aspartic acid, and Lysine at pH 3.9
● pKa 𝝰- COO- = 2.34
● pKa 𝝰- NH3+= 9.60
Solution | 2026 Update
LAB 2: Amino acids and Proteins
Purpose:
, This lab includes two experiments. The goal of the first experiment is to separate
the three components of a mixture of glycine, aspartic acid and lysine by performing an
ion-exchange chromatography. Furthermore, the aim of this experiment is to understand
the order of elution which is based upon the differences in the relative number and
strength of the basic and acidic groups in the amino acids. The goal of the second
experiment is to separate the components of a mixture of MW marker proteins by
performing an electrophoresis on a polyacrylamide gel in the presence of SDS and to also
determine the MW of an unknown protein.
Results and Discussion Ion-exchange chromatography
R1. Glycine, Aspartic acid, and Lysine at pH 3.9
Figure 1. Structures, pKa and PI of Glycine, Aspartic acid, and Lysine at pH 3.9
● pKa 𝝰- COO- = 2.34
● pKa 𝝰- NH3+= 9.60
