LS23L Week 6 - Agarose and
Polyacrylamide Gels Exam | Questions
with 100% Correct Answers | Verified |
Latest Update 2026/2027
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Terms in this set (21)
Lab Goal To determine the size and number of
subunits of a protein of known molecular
weight. There are two methods for
determining molecular weight: Gel
filtration and SDS Page. While gel
filtration gives an estimate of the "native"
weight, SDS-PAGE estimates the subunit
weights. So, using the two allows us to
determine how many of each subunit
there are.
You will also run a portion of your PCR
product from last week to determine the
quality and quantity of your PCR using
gel electrophoresis. d
, Gel Filtration A method that provides an estimate of
the molecular weight of the protein in its
"native" state.
Polyacrylamide Gels Exam | Questions
with 100% Correct Answers | Verified |
Latest Update 2026/2027
Save
Terms in this set (21)
Lab Goal To determine the size and number of
subunits of a protein of known molecular
weight. There are two methods for
determining molecular weight: Gel
filtration and SDS Page. While gel
filtration gives an estimate of the "native"
weight, SDS-PAGE estimates the subunit
weights. So, using the two allows us to
determine how many of each subunit
there are.
You will also run a portion of your PCR
product from last week to determine the
quality and quantity of your PCR using
gel electrophoresis. d
, Gel Filtration A method that provides an estimate of
the molecular weight of the protein in its
"native" state.
