CHEM 210 | Geneva
OBJECTIVE ASSESSMENT - EXAM
Biochemistry
Module 6 Exam
CHEM 210 | Geneva College
50 100%
QUESTIONS VERIFIED ANSWERS EDITION
TOPICS COVERED
Lipid Metabolism & Beta-Oxidation Membrane Lipids & Transport
Fatty Acid Synthesis & Regulation Lipid-Soluble Vitamins & Hormones
Cholesterol & Steroid Metabolism
COVER PAGE - 1
CHEM 210 Biochemistry Module 6 Exam -- 2026/2027 | Passing Score: 75% | Page 1 of 28
,SECTION 1 | Lipid Metabolism & Beta-Oxidation | Q1-Q15 | CHEM 210 Biochemistry Module 6 Exam
2026/2027
Q1 Question 1 of 50
A 24-year-old biochemistry student is studying fatty acid catabolism in hepatocytes. She observes
that a 16-carbon palmitate molecule enters the mitochondrial matrix for degradation. Which
transport mechanism is required to move the activated fatty acyl group across the inner
mitochondrial membrane?
A. Carnitine shuttle utilizing carnitine acyltransferase I and II
B. Facilitated diffusion through porin channels in the outer membrane only
C. Active transport via ATP-binding cassette transporters
D. Passive diffusion due to the hydrophobic nature of the fatty acid
Correct Answer: A
Rationale:
Long-chain fatty acyl-CoA cannot cross the inner mitochondrial membrane directly and requires the
carnitine shuttle system. Carnitine acyltransferase I on the outer mitochondrial membrane converts
acyl-CoA to acylcarnitine, which is transported across by translocase, then carnitine acyltransferase
II on the matrix side regenerates acyl-CoA.
Q2 Question 2 of 50
A 32-year-old graduate student is investigating the regulation of beta-oxidation during fasting. He
finds that malonyl-CoA levels increase after a carbohydrate-rich meal. What is the primary
regulatory effect of elevated malonyl-CoA on fatty acid oxidation?
A. Stimulation of hormone-sensitive lipase in adipose tissue
B. Inhibition of carnitine acyltransferase I preventing fatty acid entry into mitochondria
C. Activation of carnitine acyltransferase I enhancing fatty acid transport
D. Direct inhibition of the beta-oxidation enzymes in the matrix
Correct Answer: B
Rationale:
Malonyl-CoA, produced during fatty acid synthesis, acts as a feedback inhibitor of carnitine
acyltransferase I (CAT I). This prevents fatty acyl groups from entering the mitochondrial matrix for
beta-oxidation, ensuring that fatty acid synthesis and degradation do not occur simultaneously.
CHEM 210 Biochemistry Module 6 Exam -- 2026/2027 | Passing Score: 75% | Page 2 of 28
, SECTION 1 | Lipid Metabolism & Beta-Oxidation | Q1-Q15 | CHEM 210 Biochemistry Module 6 Exam
2026/2027
Q3 Question 3 of 50
A 45-year-old patient with a suspected metabolic disorder presents with hypoketotic hypoglycemia
after prolonged fasting. Biochemical testing reveals elevated levels of medium-chain dicarboxylic
acids in the urine. Deficiency of which enzyme is most likely responsible for this presentation?
A. HMG-CoA synthase
B. Long-chain acyl-CoA dehydrogenase
C. Medium-chain acyl-CoA dehydrogenase (MCAD)
D. Carnitine acyltransferase I
Correct Answer: C
Rationale:
MCAD deficiency impairs beta-oxidation of medium-chain fatty acids, leading to inability to generate
ketone bodies during fasting (hypoketotic hypoglycemia). The accumulation of medium-chain fatty
acids leads to alternative omega-oxidation, producing dicarboxylic acids that appear in the urine.
Q4 Question 4 of 50
A research biochemist is analyzing the energetics of complete beta-oxidation of palmitate (C16:0).
After calculating the yield from seven beta-oxidation cycles, he determines the total ATP
production. How many ATP molecules are produced per molecule of palmitate through
beta-oxidation and subsequent oxidative phosphorylation?
A. 129 ATP
B. 148 ATP
C. 92 ATP
D. 106 ATP
Correct Answer: D
Rationale:
Complete oxidation of palmitate yields 7 FADH2 (7 x 1.5 = 10.5 ATP), 7 NADH (7 x 2.5 = 17.5 ATP),
and 8 acetyl-CoA entering the TCA cycle (8 x 10 = 80 ATP), totaling 108 ATP. Subtracting 2 ATP for
activation gives a net yield of 106 ATP per palmitate molecule.
CHEM 210 Biochemistry Module 6 Exam -- 2026/2027 | Passing Score: 75% | Page 3 of 28
OBJECTIVE ASSESSMENT - EXAM
Biochemistry
Module 6 Exam
CHEM 210 | Geneva College
50 100%
QUESTIONS VERIFIED ANSWERS EDITION
TOPICS COVERED
Lipid Metabolism & Beta-Oxidation Membrane Lipids & Transport
Fatty Acid Synthesis & Regulation Lipid-Soluble Vitamins & Hormones
Cholesterol & Steroid Metabolism
COVER PAGE - 1
CHEM 210 Biochemistry Module 6 Exam -- 2026/2027 | Passing Score: 75% | Page 1 of 28
,SECTION 1 | Lipid Metabolism & Beta-Oxidation | Q1-Q15 | CHEM 210 Biochemistry Module 6 Exam
2026/2027
Q1 Question 1 of 50
A 24-year-old biochemistry student is studying fatty acid catabolism in hepatocytes. She observes
that a 16-carbon palmitate molecule enters the mitochondrial matrix for degradation. Which
transport mechanism is required to move the activated fatty acyl group across the inner
mitochondrial membrane?
A. Carnitine shuttle utilizing carnitine acyltransferase I and II
B. Facilitated diffusion through porin channels in the outer membrane only
C. Active transport via ATP-binding cassette transporters
D. Passive diffusion due to the hydrophobic nature of the fatty acid
Correct Answer: A
Rationale:
Long-chain fatty acyl-CoA cannot cross the inner mitochondrial membrane directly and requires the
carnitine shuttle system. Carnitine acyltransferase I on the outer mitochondrial membrane converts
acyl-CoA to acylcarnitine, which is transported across by translocase, then carnitine acyltransferase
II on the matrix side regenerates acyl-CoA.
Q2 Question 2 of 50
A 32-year-old graduate student is investigating the regulation of beta-oxidation during fasting. He
finds that malonyl-CoA levels increase after a carbohydrate-rich meal. What is the primary
regulatory effect of elevated malonyl-CoA on fatty acid oxidation?
A. Stimulation of hormone-sensitive lipase in adipose tissue
B. Inhibition of carnitine acyltransferase I preventing fatty acid entry into mitochondria
C. Activation of carnitine acyltransferase I enhancing fatty acid transport
D. Direct inhibition of the beta-oxidation enzymes in the matrix
Correct Answer: B
Rationale:
Malonyl-CoA, produced during fatty acid synthesis, acts as a feedback inhibitor of carnitine
acyltransferase I (CAT I). This prevents fatty acyl groups from entering the mitochondrial matrix for
beta-oxidation, ensuring that fatty acid synthesis and degradation do not occur simultaneously.
CHEM 210 Biochemistry Module 6 Exam -- 2026/2027 | Passing Score: 75% | Page 2 of 28
, SECTION 1 | Lipid Metabolism & Beta-Oxidation | Q1-Q15 | CHEM 210 Biochemistry Module 6 Exam
2026/2027
Q3 Question 3 of 50
A 45-year-old patient with a suspected metabolic disorder presents with hypoketotic hypoglycemia
after prolonged fasting. Biochemical testing reveals elevated levels of medium-chain dicarboxylic
acids in the urine. Deficiency of which enzyme is most likely responsible for this presentation?
A. HMG-CoA synthase
B. Long-chain acyl-CoA dehydrogenase
C. Medium-chain acyl-CoA dehydrogenase (MCAD)
D. Carnitine acyltransferase I
Correct Answer: C
Rationale:
MCAD deficiency impairs beta-oxidation of medium-chain fatty acids, leading to inability to generate
ketone bodies during fasting (hypoketotic hypoglycemia). The accumulation of medium-chain fatty
acids leads to alternative omega-oxidation, producing dicarboxylic acids that appear in the urine.
Q4 Question 4 of 50
A research biochemist is analyzing the energetics of complete beta-oxidation of palmitate (C16:0).
After calculating the yield from seven beta-oxidation cycles, he determines the total ATP
production. How many ATP molecules are produced per molecule of palmitate through
beta-oxidation and subsequent oxidative phosphorylation?
A. 129 ATP
B. 148 ATP
C. 92 ATP
D. 106 ATP
Correct Answer: D
Rationale:
Complete oxidation of palmitate yields 7 FADH2 (7 x 1.5 = 10.5 ATP), 7 NADH (7 x 2.5 = 17.5 ATP),
and 8 acetyl-CoA entering the TCA cycle (8 x 10 = 80 ATP), totaling 108 ATP. Subtracting 2 ATP for
activation gives a net yield of 106 ATP per palmitate molecule.
CHEM 210 Biochemistry Module 6 Exam -- 2026/2027 | Passing Score: 75% | Page 3 of 28
