MLT ASCP PRACTICE CERTIFICATION
SCRIPT 2026 QUESTIONS WITH
SOLUTIONS GRADED A+
◍ The serum hemolytic complement level (CH50):
A. is a measure of total complement activity
B. provides the same information as a serum factor B level
C. is detectable when any component of the classical system is congenitally
absent
D. can be calculated from the serum concentrations of the individual
components.
Answer: A.is a measure of total complement activity
◍ The indicator(s) used in the pH test region of the chemical reagent strips for
urine is/are: (Choose ALL correct answers)
A. methyl red
B. methyl blue
C. bromothymol blue
D. bromothymol red.
Answer: A & C;The indicator(s) used in the pH test region are methyl red
and bromothymol blue. Typically on most chemical reagent strips for urine
pH, with an increase in urinary pH, the indicators bromothymol blue and
methyl red, changes from orange to green and blue.
◍ Which of the following is not a standard method for measuring the specific
gravity of urine:
A. Urine reagent strips
B. Freezing point depression
C. Refractometry
D. Hydrometry.
, Answer: B;Osmolality can be measured by freezing point depression, but
not specific gravity as specified in this question. The other methods are used
to measure specific gravity in urine samples.
◍ The normal range for urine pH is:
A. 4.6 to 8.0
B. 5.0 to 6.0
C. 5.5-6.6
D. 7.35-7.45.
Answer: A;Normal urine pH varies from 4.6 to 8.0. After meals, urine
becomes more alkaline due to gastric acid secretion (alkaline tide). At night
due to shallow breathing, it becomes more acid. A high meat diet results in a
more acid urine than a vegetarian diet, due to excretion of phosphates and
sulfates.
◍ Which two of the following crystalline elements are found in acid urine:
A. Cystine
B. Triple phosphate
C. Calcium phosphate
D. Tyrosine.
Answer: A & D;Crystals and sediment found in acid urine include: cystine,
leucine, calcium oxalate, sodium urate, sulfonamide, tyrosine, uric acid, and
amorphous urates.
◍ Normal serum constituents that can rapidly increase during infection, injury
or trauma are referred to as:
A. haptens
B. acute phase reactants
C. opsonins
D. chemotaxins.
Answer: B.acute phase reactants
◍ False-positive tests for protein on a urine reagent strip may be caused by:
(Choose ALL of the correct answers)
A. Bacterial contamination
, B. Urine that has remained at room temperature for an extended period of
time
C. Alkaline medication
D. Ascorbic acid.
Answer: A-C;Bacterial contamination, urine that has remained at room
temperature for an extended period of time, and the presence of alkaline
medication are all factors which can cause false positives on the protein pad
of a urine reagent strip.
◍ Bence-Jones proteinuria can be seen in all of the following conditions
except:
A. Amyloidosis
B. Nephrotic syndrome
C. Multiple myeloma
D. Macroglobulinemia.
Answer: B;Bence-Jones proteins are monoclonal light chains excreted in the
urine, seen with multiple myeloma, and other neoplasms of lymphoid cells.
Small amounts can be missed by urine dipsticks, which are more sensitive to
albumin than globulins. They are best detected by urine protein
electrophoresis, immunoelectrophoresis, or immunofixation. Large amounts
cause renal tubular damage, resulting in myeloma kidney. Bence-Jones
proteins have unusual heat solubility properties, in that they precipitate
between 50- 60 degrees C and redisolve at 90 -100 degrees C. Historical
detection methods often utilized this unusual property.
◍ Chronic carriers of HBV:
A. have chronic symptoms of hepatitis
B. continue to carry HBV
C. do not transmit infection
D. carry HBV but are not infectious.
Answer: B.continue to carry HBV
◍ Which of the following cytokines is produced by an activated CD8 cytotoxic
T cell?
, A. interleukin 13 (IL-13)
B. interleukin 5 (IL-5)
C. stromal cell-derived factor 1 (SDF-1)
D. interferon gamma (IFNγ).
Answer: D.interferon gamma (IFNγ)
◍ T/F:The crystals that are seen in this image are calcium oxalate..
Answer: False; These are leucine crystals.
◍ Identify the urine sediment elements shown by the arrow:
A. Cholesterol crystals
B. Uric acid crystals
C. Amorphous urate crystals
D. Cystine crystals.
Answer: D;Cystine crystals are characteristically seen as colorless
hexagonal plates in acid urine. They may be confused with hexagonal uric
acid crystals. They can be differentiated from uric acid by their solubility in
dilute hydrochloric acid versus crystalline uric acid, which is not soluble in
dilute hydrochloric acid. The cyanide-nitroprusside test can be used to
confirm the presence of cystine in urine. Cystine crystals are not present in
normal urine.
◍ The IgM molecule is a:
A. dimer
B. trimer
C. tetramer
D. pentamer.
Answer: D.pentamer
◍ Treatment of IgG with papain results in how many fragments from each
immunoglobulin molecule?
A. 2
B. 3
C. 4
D. 5.
SCRIPT 2026 QUESTIONS WITH
SOLUTIONS GRADED A+
◍ The serum hemolytic complement level (CH50):
A. is a measure of total complement activity
B. provides the same information as a serum factor B level
C. is detectable when any component of the classical system is congenitally
absent
D. can be calculated from the serum concentrations of the individual
components.
Answer: A.is a measure of total complement activity
◍ The indicator(s) used in the pH test region of the chemical reagent strips for
urine is/are: (Choose ALL correct answers)
A. methyl red
B. methyl blue
C. bromothymol blue
D. bromothymol red.
Answer: A & C;The indicator(s) used in the pH test region are methyl red
and bromothymol blue. Typically on most chemical reagent strips for urine
pH, with an increase in urinary pH, the indicators bromothymol blue and
methyl red, changes from orange to green and blue.
◍ Which of the following is not a standard method for measuring the specific
gravity of urine:
A. Urine reagent strips
B. Freezing point depression
C. Refractometry
D. Hydrometry.
, Answer: B;Osmolality can be measured by freezing point depression, but
not specific gravity as specified in this question. The other methods are used
to measure specific gravity in urine samples.
◍ The normal range for urine pH is:
A. 4.6 to 8.0
B. 5.0 to 6.0
C. 5.5-6.6
D. 7.35-7.45.
Answer: A;Normal urine pH varies from 4.6 to 8.0. After meals, urine
becomes more alkaline due to gastric acid secretion (alkaline tide). At night
due to shallow breathing, it becomes more acid. A high meat diet results in a
more acid urine than a vegetarian diet, due to excretion of phosphates and
sulfates.
◍ Which two of the following crystalline elements are found in acid urine:
A. Cystine
B. Triple phosphate
C. Calcium phosphate
D. Tyrosine.
Answer: A & D;Crystals and sediment found in acid urine include: cystine,
leucine, calcium oxalate, sodium urate, sulfonamide, tyrosine, uric acid, and
amorphous urates.
◍ Normal serum constituents that can rapidly increase during infection, injury
or trauma are referred to as:
A. haptens
B. acute phase reactants
C. opsonins
D. chemotaxins.
Answer: B.acute phase reactants
◍ False-positive tests for protein on a urine reagent strip may be caused by:
(Choose ALL of the correct answers)
A. Bacterial contamination
, B. Urine that has remained at room temperature for an extended period of
time
C. Alkaline medication
D. Ascorbic acid.
Answer: A-C;Bacterial contamination, urine that has remained at room
temperature for an extended period of time, and the presence of alkaline
medication are all factors which can cause false positives on the protein pad
of a urine reagent strip.
◍ Bence-Jones proteinuria can be seen in all of the following conditions
except:
A. Amyloidosis
B. Nephrotic syndrome
C. Multiple myeloma
D. Macroglobulinemia.
Answer: B;Bence-Jones proteins are monoclonal light chains excreted in the
urine, seen with multiple myeloma, and other neoplasms of lymphoid cells.
Small amounts can be missed by urine dipsticks, which are more sensitive to
albumin than globulins. They are best detected by urine protein
electrophoresis, immunoelectrophoresis, or immunofixation. Large amounts
cause renal tubular damage, resulting in myeloma kidney. Bence-Jones
proteins have unusual heat solubility properties, in that they precipitate
between 50- 60 degrees C and redisolve at 90 -100 degrees C. Historical
detection methods often utilized this unusual property.
◍ Chronic carriers of HBV:
A. have chronic symptoms of hepatitis
B. continue to carry HBV
C. do not transmit infection
D. carry HBV but are not infectious.
Answer: B.continue to carry HBV
◍ Which of the following cytokines is produced by an activated CD8 cytotoxic
T cell?
, A. interleukin 13 (IL-13)
B. interleukin 5 (IL-5)
C. stromal cell-derived factor 1 (SDF-1)
D. interferon gamma (IFNγ).
Answer: D.interferon gamma (IFNγ)
◍ T/F:The crystals that are seen in this image are calcium oxalate..
Answer: False; These are leucine crystals.
◍ Identify the urine sediment elements shown by the arrow:
A. Cholesterol crystals
B. Uric acid crystals
C. Amorphous urate crystals
D. Cystine crystals.
Answer: D;Cystine crystals are characteristically seen as colorless
hexagonal plates in acid urine. They may be confused with hexagonal uric
acid crystals. They can be differentiated from uric acid by their solubility in
dilute hydrochloric acid versus crystalline uric acid, which is not soluble in
dilute hydrochloric acid. The cyanide-nitroprusside test can be used to
confirm the presence of cystine in urine. Cystine crystals are not present in
normal urine.
◍ The IgM molecule is a:
A. dimer
B. trimer
C. tetramer
D. pentamer.
Answer: D.pentamer
◍ Treatment of IgG with papain results in how many fragments from each
immunoglobulin molecule?
A. 2
B. 3
C. 4
D. 5.
