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CHT Exam Prep Questions & Answers update

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Prepare for the ASHI/ACHI Certified Histocompatibility Technologist (CHT) exam with 2026 updated practice questions covering HLA typing, crossmatching, flow cytometry, PCR, immunogenetics, and ASHI standards. Multiple-choice format with accurate answers for transplant immunology certification.

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CHT EXAM PREP EXAM, QUESTIONS WITH ACCU
b b b b b b




RATE ANswers MULTIPLE CHOICES (-
b b b b




2026 update) b




b WhatbdobASHIbStandardsbsaybaboutbrunningbbothbthebopticalbandbfluorescentbstandardsbforbflowbcytometr
y?
A. Runbeachbtimebthebinstrumentbisbturnedbon
B. Runbanybtimebmaintenancebhasboccurred
C. Runbanybtimebproblemsbhaveboccurredbthatbcouldbpotentiallybaffectbinstrumentbfunction
D. Allbofbthebaboveb-bANS>>D.bAllbofbthebabove


ThebinitialbrecipientbworkupbforbabkidneybtransplantbincludesballbofbthebfollowingbEXCEPT?
A. EstablishingbthebcausebofbthebESRDb(EndbStagebRenalbDisease),bifbpossible
B. MeasuringbthebGFRb(glomerularbfiltrationbrate)
C. Performingbabhealthbexaminationbandbbloodbwork
D. DeterminingbifbthebpatientbisbabU.S.bcitizen
Determiningbthebpsychologicalbandbsocialbaspectsbofbthebcandidateb-
b ANS>>D.bDeterminingbifbthebpatientbisbabU.S.bcitizen


Oncebabcandidatebhasbbeenbapprovedbforbabtransplant,bwhatbhistocompatibilitybtest(s)bis/arebcarriedbout?
A. HLAbtyping
B. ScreeningbforbantibodiesbdirectedbtobforeignbHLA
C. ABObtyping
D. HLAbtypingbandbantibodybscreeningbHLAbtyping,bHLAbantibodybscreening,bandbABObtypingb-
b ANS>>D.bHLAbtypingbandbantibodybscreeningbHLAbtyping,bHLAbantibodybscreening,bandbABObtyping

,AllbofbthebfollowingbarebtruebaboutblivingbdonorsbEXCEPT?
A. Mustbbeboverb16byearsbofbage
B. Mustbbebatblowbriskbforbanybcomplicationsbassociatedbwithbtransplantation
C. Mustbbebmakingbthisbdecisionbfreelybandbforbaltruisticbreasons
D. Mustbnotbexhibitbanybbehaviorsbthatbmaybincreasebthebriskbforbdiseasebtransmission
E. MustbsignbanbInformedbConsentbformb-bANS>>A.bMustbbeboverb16byearsbofbage


AllbofbthebfollowingbarebtruebaboutbunacceptablebantigensbEXCEPT?
A. IdentifiedbbybthebpresencebofbantibodiesbinbthebtransplantbcandidatebdirectedbagainstbforeignbHLA
B. EnteredbintobUNETbforbabtransplantbcandidate
C. Usedbtobidentifybunacceptablebdonors
D. CannotbbebalteredboncebtheybarebinitiallybenteredbintobUNET
E. Theirbpresencebcanbbebalteredbbybvaccinationborbinfectionb-
ANS>>D.bCannotbbebalteredboncebtheybarebinitiallybenteredbintobUNET
b




Whatbgroupboverseesbthebinitialbactivitiesbrelatedbtobdeceasedbdonorsblikebobtainingbfamilybconsentbforbdo
nation,boverseeingbthebclinicalbmanagementbofbthebdonor,bandbbeginningbtheborganballocationbprocedures
?
A. UNOS
B. OrganbProcurementbOrganizationb(OPO)
C. Candidate'sbtransplantbprogram
D. HLAblaboratory
E. Localbhospitalbwherebthebdonorbisblocatedb-bANS>>B.bOrganbProcurementbOrganizationb(OPO)


Thebvirtualbcrossmatchbbetweenbthebdeceasedbdonorbandbpotentialbrecipientsbconsidersballbofbthebfollowin
gbEXCEPT?
A. Unacceptablebantigens
B. CREGbgroupsbandbepitopes
C. AntibodiesbdirectedbtobDRA
D. Possiblebfalsebpositivebantibodybreactionsbinbpatient'sbmedicalbhistory
E. Pastbpositive,bcurrentbnegativebantibodiesb-bANS>>C.bAntibodiesbdirectedbtobDRA
30b/b36

,Abdiscrepancybbetweenbthebvirtualbcrossmatchbandbthebphysicalbcrossmatchbmaybbebcausedbbybwhichbofbt
hebfollowing?
A. Technicalbproblembwhenbperformingbphysicalbcrossmatch
B. PresencebofbIgMbinbserum
C. Denaturedbantigenbonbsinglebantigenbbeadsbusedbforbantibodybtest
D. Mismatchedballelebnotbonbpanelbusedbforbantibodybtest
E. Allbofbthebaboveb-bANS>>E.bAllbofbthebabove


WhichbofbthebfollowingbarebrequiredbbybASHI'sbstandardsbforbtransplantbsupport?
A. Protocolsbforbpatientsbatbhighbriskbforballograftbrejection
B. Policybonbrequirementsbforbthebsensitivitybandbspecificitybofbthebtestbsystembrequiredbtobsupportb
clinicalbtransplantbprotocols
C. PolicybtobperiodicallybscreenbserumbsamplesbfrombeachbtransplantbpatientbforbantibodybtobHLAb
antigens,bincludingbthebfrequencybofbscreeningbserumbsamples
D. Policybforbstoragebandbmaintenancebofbrelevantbtransplantbsamples
E. AllbofbthesebarebrequiredbbybASHIbstandards.b-bANS>>E.bAllbofbthesebarebrequiredbbybASHIbstandards.


PreformedbHLAbantibodiesbinbthebtransplantbcandidatebthatbarebdirectedbagainstbmismatchedbHLAbinbabpot
entialbdonorbcanbcause?
A. Hyperacutebrejection
B. Tbcellbmediatedbrejection
C. Abdelaybinbengraftmentbofbthebkidney
D. Chronicbrejection
E. Allbofbthebaboveb-bANS>>A.bHyperacutebrejection


Wherebinbthebcellbdoesbtranscriptionb(transferringbinformationbfrombDNAbtobmRNA)btakebplace?
A. Nucleus
B. Cytoplasm
C. Endoplasmicbreticulum
D. Golgibapparatus


31b/b36

, E. Lysosomeb-bANS>>A.bNucleus


WhichbofbthebfollowingbisbNOTbtruebaboutbthebgeneticbcode?
A. Eachbcodonbisb3blettersblong
B. Therebarebcodonsbthatb"tell"bthebribosomebtobterminatebsynthesis
C. Eachbaminobacidbisbspecifiedbbybonlybabsinglebcodon
D. AbcodonbinbthebmRNAbmightbbebGUA,bcorrespondingbtobGTAbinbthebDNA
E. Ribosomesbreadbthebcodebduringbproteinbsynthesisb-
b ANS>>C.bEachbaminobacidbisbspecifiedbbybonlybabsinglebcodon


HowbmanybhydrogenbbondsblinkbthebnucleotidebbasesbGbandbTbinbdoublebstrandedbDNA?
A. 4
B. 3
C. 2
D. 1
E. 0b-bANS>>E.b0


Whichbaminobacidbcanbformbdisulfidebbonds?
A. Leucine
B. Cysteine
C. Phenylalanine
D. Valine
E. Glutamicbacidb-bANS>>B.bCysteine


Whatbisbthebtermbusedbtobcharacterizebanbaminobacidbthatbprefersbtobbebonbthebinteriorbofbabprotein,bhiddenb
awaybfrombwater?
A. Polar
B. Hydrophobic
C. Positivelybcharged
D. Negativelybcharged
E. Hydrophilicb-bANS>>B.bHydrophobic

32b/b36

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