LATG 10
Which of the following is a sign of good maternal care and good pup health?
A. A couple of the pups are larger than the others.
B. The pups have visible milk spots.
C. Pups are scattered about the cage.
D. The dam has a bulging abdomen. - answerB. The pups have visible milk spots.
Which of the following best describes genetic engineering?
A. Changing the phenotype of an animal
B. Introducing foreign DNA into embryonic stem cells
C. Altering the genetic information of cells
D. Manipulating DNA molecules to alter their composition - answerD. Manipulating DNA
molecules to alter their composition
Which of the following order of steps should be followed to produce gene-targeted
animals?
A. Mating with vasectomized male; injection of superovulating hormone (PMSG);
injection of egg-releasing hormone (hCG); harvesting of embryo
B. Injection of superovulating hormone (PMSG); injection of egg-releasing hormone
(hCG); mating with stud male; harvesting of embryo
C. Injection of superovulating hormone (PMSG); injection of egg-releasing hormone
(hCG); mating with vasectomized male; harvesting of embryo
D. Injection of superovulating hormone (PMSG); mating with stud male; injection of egg-
releasing hormone (hCG); harvesting of embryo - answerB. Injection of superovulating
hormone (PMSG); injection of egg-releasing hormone (hCG); mating with stud male;
harvesting of embryo
Genetically engineered mice are often transported between institutions which often
results in:
A. the need for more frequent sentinel testing.
B. a simplification of the required paperwork.
C. the need for specialized transport vehicles.
D. more frequent genotyping mistakes. - answerA. the need for more frequent sentinel
testing.
Among transgenic mice, what does the generation called F2 represent?
A. The founder of a line
B. The offspring of the founder of a line
C. The second generation of the founder of a line
D. The brother-sister mating of founders of a line - answerC. The second generation of
the founder of a line
, What best describes a congenic strain?
A. The result of backcrossing for 10 generations
B. The result of consecutive matings of two hemizygous transgenic parents
C. The result of consecutive matings of two transgenic homozygous parents
D. The result of brother-sister mating of transgenics - answerA. The result of
backcrossing for 10 generations
Which factor can lead to the embryos collected from donor females for injection of
modified DNA not being at the appropriate cell stage of development?
A. Light cycle was disrupted.
B. Superovulation caused overproduction of eggs.
C. Females were sterile.
D. The stud males were from a different strain of mice than the females. - answerA.
Light cycle was disrupted.
Which of the following best describes "alleles"?
A. Copies of the same gene
B. The genes produced by insertion of a DNA fragment from another species
C. Genes found on all chromosomes of a species
D. Different DNA fragments found on the same chromosome - answerA. Copies of the
same gene
Using the pronuclear injection technique, what is the average percentage of offspring
carrying the transgene?
A. 5%
B. 50%
C. 80%
D. 20% - answerD. 20%
If only one allele is mutated using CRISPR/Cas9, what is the resulting animal known
as?
A. A mosaic animal
B. A mixed gene mouse
C. A transgenic hybrid
D. A chimera - answerA. A mosaic animal
Which of the following processes describes recovering a mouse line?
A. Founders resulting from pronuclear injection are mated to wild type to produce
offspring that have the transgene.
B. Chimeras are genotyped and the hemizygous ones are mated together.
C. Frozen embryos are thawed and implanted in a pseudopregnant female.
D. Frozen embryos are thawed and grown in vitro. - answerC. Frozen embryos are
thawed and implanted in a pseudopregnant female.
If a genetically modified mouse is dying and cannot be saved, it is important to attempt
to save which of the following?
Which of the following is a sign of good maternal care and good pup health?
A. A couple of the pups are larger than the others.
B. The pups have visible milk spots.
C. Pups are scattered about the cage.
D. The dam has a bulging abdomen. - answerB. The pups have visible milk spots.
Which of the following best describes genetic engineering?
A. Changing the phenotype of an animal
B. Introducing foreign DNA into embryonic stem cells
C. Altering the genetic information of cells
D. Manipulating DNA molecules to alter their composition - answerD. Manipulating DNA
molecules to alter their composition
Which of the following order of steps should be followed to produce gene-targeted
animals?
A. Mating with vasectomized male; injection of superovulating hormone (PMSG);
injection of egg-releasing hormone (hCG); harvesting of embryo
B. Injection of superovulating hormone (PMSG); injection of egg-releasing hormone
(hCG); mating with stud male; harvesting of embryo
C. Injection of superovulating hormone (PMSG); injection of egg-releasing hormone
(hCG); mating with vasectomized male; harvesting of embryo
D. Injection of superovulating hormone (PMSG); mating with stud male; injection of egg-
releasing hormone (hCG); harvesting of embryo - answerB. Injection of superovulating
hormone (PMSG); injection of egg-releasing hormone (hCG); mating with stud male;
harvesting of embryo
Genetically engineered mice are often transported between institutions which often
results in:
A. the need for more frequent sentinel testing.
B. a simplification of the required paperwork.
C. the need for specialized transport vehicles.
D. more frequent genotyping mistakes. - answerA. the need for more frequent sentinel
testing.
Among transgenic mice, what does the generation called F2 represent?
A. The founder of a line
B. The offspring of the founder of a line
C. The second generation of the founder of a line
D. The brother-sister mating of founders of a line - answerC. The second generation of
the founder of a line
, What best describes a congenic strain?
A. The result of backcrossing for 10 generations
B. The result of consecutive matings of two hemizygous transgenic parents
C. The result of consecutive matings of two transgenic homozygous parents
D. The result of brother-sister mating of transgenics - answerA. The result of
backcrossing for 10 generations
Which factor can lead to the embryos collected from donor females for injection of
modified DNA not being at the appropriate cell stage of development?
A. Light cycle was disrupted.
B. Superovulation caused overproduction of eggs.
C. Females were sterile.
D. The stud males were from a different strain of mice than the females. - answerA.
Light cycle was disrupted.
Which of the following best describes "alleles"?
A. Copies of the same gene
B. The genes produced by insertion of a DNA fragment from another species
C. Genes found on all chromosomes of a species
D. Different DNA fragments found on the same chromosome - answerA. Copies of the
same gene
Using the pronuclear injection technique, what is the average percentage of offspring
carrying the transgene?
A. 5%
B. 50%
C. 80%
D. 20% - answerD. 20%
If only one allele is mutated using CRISPR/Cas9, what is the resulting animal known
as?
A. A mosaic animal
B. A mixed gene mouse
C. A transgenic hybrid
D. A chimera - answerA. A mosaic animal
Which of the following processes describes recovering a mouse line?
A. Founders resulting from pronuclear injection are mated to wild type to produce
offspring that have the transgene.
B. Chimeras are genotyped and the hemizygous ones are mated together.
C. Frozen embryos are thawed and implanted in a pseudopregnant female.
D. Frozen embryos are thawed and grown in vitro. - answerC. Frozen embryos are
thawed and implanted in a pseudopregnant female.
If a genetically modified mouse is dying and cannot be saved, it is important to attempt
to save which of the following?
