LATG Chapter 11. Gnotobiotics
What does the term "gnotobiotics" mean?
a. Precarious life
b. Well-known life
c. Unnatural biology
d. Abnormal biology - answerb
Animals that were originally germ-free but were given a known group of bacteria are
called ______________ animals.
a. axenic
b. specific pathogen free
c. defined flora
d. normal - answerc
Animals that have a normal intestinal flora but are free of particular disease-causing
organisms are called:
a. axenic
b. specific pathogen free
c. defined flora
d. normal - answerb
What is the term for using isolator caging to prevent microorganisms from reaching the
animals housed inside?
a. Prevention
b. Containment
c. Inclusion
d. Exclusion - answerd
What is another name for an axenic animal?
a. Germ-free animal
b. Clean-gut animal
c. Normal flora animal
d. Aflora animal - answera
What is a difference between hysterectomy derivation and embryo transfer?
a. In embryo transfer, the embryos are at an early stage of development; in
hysterectomy derivation, the fetuses are at term.
b. In embryo transfer, the embryos are transferred to a surrogate mother; in
hysterectomy derivation, the fetuses are returned to their biological mother.
c. In embryo transfer, the pups are removed at birth and adopted by a foster mother; in
hysterectomy derivation, the pups are bottle-fed as orphans.
d. None of the above - answera
, What is a factor that influences success in producing axenic pups by hysterectomy
derivation?
a. The number of pups in the foster mother's litter
b. The use of an axenic father
c. The timing of the hysterectomy
d. The type of anesthesia used - answerc
The foster mother used to raise axenic mice should have had her own litter no more
than ___ days before the donor's pups are put in her cage.
a. 1
b. 2
c. 5
d. 7 - answerb
To obtain axenic pups, the hysterectomy on the donor female is best done __________
hours prior to natural parturition.
a. 1-2
b. 4-6
c. 6-8
d. 10-12 - answerc
Which of the following would help maintain genetic integrity when producing axenic
pups by hysterectomy derivation?
a. Use aseptic technique during the hysterectomy procedure.
b. Make sure that all the animals used in the process have the same genetic
background.
c. Choose foster mothers whose litter has been weaned and the pups removed.
d. Use a donor female and foster mother with different coat colors. - answerd
Axenic animals have an intestinal wall that is _____________ than in animals with a
normal microflora.
a. thicker
b. thinner
c. less fibrous
d. more porous - answerb
Which characteristic is true of axenic animals as compared to animals with normal
microflora?
a. Axenic animals have lower levels of tissue enzymes.
b. Axenic animals have thicker intestinal walls.
c. Axenic animals have a larger thymus.
d. Axenic animals have a shorter life span. - answera
Which characteristic is true of axenic animals as compared to animals with normal
microflora?
What does the term "gnotobiotics" mean?
a. Precarious life
b. Well-known life
c. Unnatural biology
d. Abnormal biology - answerb
Animals that were originally germ-free but were given a known group of bacteria are
called ______________ animals.
a. axenic
b. specific pathogen free
c. defined flora
d. normal - answerc
Animals that have a normal intestinal flora but are free of particular disease-causing
organisms are called:
a. axenic
b. specific pathogen free
c. defined flora
d. normal - answerb
What is the term for using isolator caging to prevent microorganisms from reaching the
animals housed inside?
a. Prevention
b. Containment
c. Inclusion
d. Exclusion - answerd
What is another name for an axenic animal?
a. Germ-free animal
b. Clean-gut animal
c. Normal flora animal
d. Aflora animal - answera
What is a difference between hysterectomy derivation and embryo transfer?
a. In embryo transfer, the embryos are at an early stage of development; in
hysterectomy derivation, the fetuses are at term.
b. In embryo transfer, the embryos are transferred to a surrogate mother; in
hysterectomy derivation, the fetuses are returned to their biological mother.
c. In embryo transfer, the pups are removed at birth and adopted by a foster mother; in
hysterectomy derivation, the pups are bottle-fed as orphans.
d. None of the above - answera
, What is a factor that influences success in producing axenic pups by hysterectomy
derivation?
a. The number of pups in the foster mother's litter
b. The use of an axenic father
c. The timing of the hysterectomy
d. The type of anesthesia used - answerc
The foster mother used to raise axenic mice should have had her own litter no more
than ___ days before the donor's pups are put in her cage.
a. 1
b. 2
c. 5
d. 7 - answerb
To obtain axenic pups, the hysterectomy on the donor female is best done __________
hours prior to natural parturition.
a. 1-2
b. 4-6
c. 6-8
d. 10-12 - answerc
Which of the following would help maintain genetic integrity when producing axenic
pups by hysterectomy derivation?
a. Use aseptic technique during the hysterectomy procedure.
b. Make sure that all the animals used in the process have the same genetic
background.
c. Choose foster mothers whose litter has been weaned and the pups removed.
d. Use a donor female and foster mother with different coat colors. - answerd
Axenic animals have an intestinal wall that is _____________ than in animals with a
normal microflora.
a. thicker
b. thinner
c. less fibrous
d. more porous - answerb
Which characteristic is true of axenic animals as compared to animals with normal
microflora?
a. Axenic animals have lower levels of tissue enzymes.
b. Axenic animals have thicker intestinal walls.
c. Axenic animals have a larger thymus.
d. Axenic animals have a shorter life span. - answera
Which characteristic is true of axenic animals as compared to animals with normal
microflora?
