Bio 111: Tamu Exam 2 review
Questions with All Complete
Solutions.
When biologists wish to study the internal ultrastructure of cells, they can achieve the finest
resolution by using - Answer a transmission electronic microscope
The advantage of light microscopy over electron microscopy is that - Answer light microscopy
allows one to view dynamic processes in living cells
A primary objective of cell fractionation is to - Answer separate the major organelles so that
their particular functions can be determined
In the fractionation of homogenized cells using centrifugation, the primary factor that
determines whether a specific cellular component ends up in the supernatant or the pellet is -
Answer the size and weight of the component.
Which of the following correctly lists the order in which cellular components will be found in the
pellet when homogenized cells are treated with increasingly rapid spins in a centrifuge? -
Answer nucleus, mitochondria, ribosomes
Green fluorescent protein (GFP) can be used to fluorescently label a specific protein in cells by
genetically engineering cells to synthesize the target protein fused to GFP. What is the
advantage of using GFP fusions to visualize specific proteins, instead of staining cells with
fluorescently labeled probes that bind to the target protein? - Answer GFP fusions enable
one to track changes in the location of the protein in living cells; staining usually requires
preserved cells
What is the reason that a modern electron microscope (TEM) can resolve biological images to
the subnanometer level, as opposed to tens of nanometers achievable for the best super-
resolution light microscope? - Answer Electron beams have much shorter wavelengths than
visible light
What technique would be most appropriate to use to observe the movements of condensed
chromosomes during cell division - Answer light microscopy
All of the following are part of a prokaryotic cell except - Answer an endoplasmic reticulum
, The volume enclosed by the plasma membrane of plant cells is often much larger than the
corresponding volume in animal cells. The most reasonable explanation for this observation is
that - Answer plant cells contain a large vacuole that reduces the volume of the cytoplasm
A mycoplasma is an organism with a diameter between 0.1 and 1.0 µm. What does the
organism's size tell you about how it might be classified? - Answer It could be a very small
bacterium.
Which of the following is a major cause of the size limits for certain types of cells? - Answer
the need for a surface area of sufficient area to support the cell's metabolic needs
Which of the following statements concerning bacteria and archaea cells is correct? - Answer
Archaea cells contain small membrane-enclosed organelles; bacteria do not
The evolution of eukaryotic cells most likely involved - Answer endosymbiosis of an aerobic
bacterium in a larger host cell-the endosymbiont evolved into mitochondria
Prokaryotes are classified as belonging to two different domains. What are the domains? -
Answer Bacteria and Archaea
If radioactive deoxythymidine triphosphate (dTTP) is added to a culture of rapidly growing
bacterialcells, where in the cell would you expect to find the greatest concentration of
radioactivity? - Answer nucleoid
Which organelle or structure is absent in plant cells? - Answer centrosomes
Large numbers of ribosomes are present in cells that specialize in producing which of the
following molecules - Answer proteins
The nuclear lamina is an array of filaments on the inner side of the nuclear membrane. If a
method were found that could cause the lamina to fall into disarray, what would you expect to
be the most likely consequence? - Answer a change in the shape of the nucleus
Recent evidence shows that when chromosomes decondense during interphase, their DNA
molecules do not intermingle. Instead, they occupy distinct territories within the nucleus.
Considering the structure and location of the following structures, which is most likely to be
involved in chromosomelocation? - Answer the nuclear matrix
A cell with a predominance of free ribosomes is most likely - Answer producing primarily
cytoplasmic proteins
Questions with All Complete
Solutions.
When biologists wish to study the internal ultrastructure of cells, they can achieve the finest
resolution by using - Answer a transmission electronic microscope
The advantage of light microscopy over electron microscopy is that - Answer light microscopy
allows one to view dynamic processes in living cells
A primary objective of cell fractionation is to - Answer separate the major organelles so that
their particular functions can be determined
In the fractionation of homogenized cells using centrifugation, the primary factor that
determines whether a specific cellular component ends up in the supernatant or the pellet is -
Answer the size and weight of the component.
Which of the following correctly lists the order in which cellular components will be found in the
pellet when homogenized cells are treated with increasingly rapid spins in a centrifuge? -
Answer nucleus, mitochondria, ribosomes
Green fluorescent protein (GFP) can be used to fluorescently label a specific protein in cells by
genetically engineering cells to synthesize the target protein fused to GFP. What is the
advantage of using GFP fusions to visualize specific proteins, instead of staining cells with
fluorescently labeled probes that bind to the target protein? - Answer GFP fusions enable
one to track changes in the location of the protein in living cells; staining usually requires
preserved cells
What is the reason that a modern electron microscope (TEM) can resolve biological images to
the subnanometer level, as opposed to tens of nanometers achievable for the best super-
resolution light microscope? - Answer Electron beams have much shorter wavelengths than
visible light
What technique would be most appropriate to use to observe the movements of condensed
chromosomes during cell division - Answer light microscopy
All of the following are part of a prokaryotic cell except - Answer an endoplasmic reticulum
, The volume enclosed by the plasma membrane of plant cells is often much larger than the
corresponding volume in animal cells. The most reasonable explanation for this observation is
that - Answer plant cells contain a large vacuole that reduces the volume of the cytoplasm
A mycoplasma is an organism with a diameter between 0.1 and 1.0 µm. What does the
organism's size tell you about how it might be classified? - Answer It could be a very small
bacterium.
Which of the following is a major cause of the size limits for certain types of cells? - Answer
the need for a surface area of sufficient area to support the cell's metabolic needs
Which of the following statements concerning bacteria and archaea cells is correct? - Answer
Archaea cells contain small membrane-enclosed organelles; bacteria do not
The evolution of eukaryotic cells most likely involved - Answer endosymbiosis of an aerobic
bacterium in a larger host cell-the endosymbiont evolved into mitochondria
Prokaryotes are classified as belonging to two different domains. What are the domains? -
Answer Bacteria and Archaea
If radioactive deoxythymidine triphosphate (dTTP) is added to a culture of rapidly growing
bacterialcells, where in the cell would you expect to find the greatest concentration of
radioactivity? - Answer nucleoid
Which organelle or structure is absent in plant cells? - Answer centrosomes
Large numbers of ribosomes are present in cells that specialize in producing which of the
following molecules - Answer proteins
The nuclear lamina is an array of filaments on the inner side of the nuclear membrane. If a
method were found that could cause the lamina to fall into disarray, what would you expect to
be the most likely consequence? - Answer a change in the shape of the nucleus
Recent evidence shows that when chromosomes decondense during interphase, their DNA
molecules do not intermingle. Instead, they occupy distinct territories within the nucleus.
Considering the structure and location of the following structures, which is most likely to be
involved in chromosomelocation? - Answer the nuclear matrix
A cell with a predominance of free ribosomes is most likely - Answer producing primarily
cytoplasmic proteins
