ACTUAL EXAM PAPER QUESTIONS AND
SOLUTIONS GRADED A PLUS
◉ Simple staining is used mostly for...
Answer: A quick observance
◉ Simple staining uses __________ dye.
Answer: Positively charged
◉ Acid fast stain is used for...
Answer: bacteria resistant to traditional staining; mostly for
mycobacterium tuberculosis
◉ Resolution
Answer: distance between two objects at which the objects can still
be seen as separate
◉ Contrast
Answer: difference in light absorbance between 2 areas
, ◉ Dark field microscopy light
Answer: reflects light off of specimen at an angle
◉ Can you see intracellular structures with a dark field microscope?
Answer: No
◉ What microscope can capture in 3-D?
Answer: Confocal
◉ What are the two types of electron microscopes?
Answer: transmission electron microscope (TEM) and scanning
electron microscope (SEM)
◉ What is the difference between TEM and SEM?
Answer: TEM passes through the sample, SEM reflects off the
surface
◉ Gram staining was developed by
Answer: Hans Christian Gram in 1884
◉ Gram-positive bacteria shows that