CHT EXAM COMPLETE STUDY GUIDE
QUESTIONS WITH CORRECT ANSWERS
It is possible to convert the plasma
| | | | | |
into serum by recalcifying the plasma and allowing coagulation to occur.
| | | | | | | | | |
|- CORRECT ANSWER✔✔-Calcium is added to the plasma to form a
| | | | | | | | | | |
complex with the citrate present in the anti-coagulant. This restores a
| | | | | | | | | | |
normal
ionized calcium level allowing clotting to occur.
| | | | | |
Polyspecific antiserum |
can be______ with lymphocytes to determine if there is a single
| | | | | | | | | | |
antibody or multiple | |
antibodies. - CORRECT ANSWER✔✔-absorbed | | |
Another application of lymphocyte
| | |
absorption is to use the patient's own cells to remove_____. Absorption
| | | | | | | | | |
can be performed
| | |
at cold or warm temperatures depending on the type of antibody. -
| | | | | | | | | | | |
CORRECT ANSWER✔✔-autoantibody |
,_________ are typically | |
cold reacting (4oC) and________are typically warm reacting (22-37oC). -
| | | | | | | |
CORRECT ANSWER✔✔-Autoantibodies
| |
| B cell antibodies
| |
Patient serum may contain autoantibodies and/or IgM antibodies that
| | | | | | | | |
may interfere the binding of
| | | |
anti-HLA IgG antibodies to the antigen. In complement dependent
| | | | | | | | |
assays (CDC), IgM antibodies | | |
can activate complement; potentially leading to ___________. -
| | | | | | | |
CORRECT ANSWER✔✔-false positive reactivity.
| | |
DTT - CORRECT ANSWER✔✔-dithiothreitol, also known as Cleland's
| | | | | | | |
Reagent
DTT inactivates IgM molecules by cleaving the _________ in the
| | | | | | | | |
pentamer structure. - CORRECT ANSWER✔✔-disulphide bonds
| | | | |
Heat inactivation works by cleaving the disulphide bonds in the
| | | | | | | | |
pentamer structure, but is less specific than _____. - CORRECT
| | | | | | | | | |
ANSWER✔✔-DTT.
ALA - CORRECT ANSWER✔✔-therapeutic antilymphocyte
| | | |
,antibodies
Thymoglobulin - CORRECT ANSWER✔✔-purified, pasteurized, gamma| | | | | |
immune globulin, |
obtained by immunization of rabbits with human thymocytes
| | | | | | |
OKT3 - CORRECT ANSWER✔✔-a murine IgG monoclonal antibody
| | | | | | | |
directed against CD3 | |
If these therapeutic antilymphocyte
| | |
antibodies are present in the patient serum, they can cause_______
| | | | | | | | | |
reactivity in |
T cell cytotoxic assays. - CORRECT ANSWER✔✔-false positive
| | | | | | |
ALA removal is accomplished by absorption using _____coated with
| | | | | | | | |
anti-rabbit or anti-mouse immunoglobulin for Thymoblobulin or OKT3,
| | | | | | | |
respectively. - CORRECT ANSWER✔✔-magnetic beads
| | | |
serum preparations - CORRECT ANSWER✔✔-1. Recalcificiation of
| | | | | | |
plasma
2. Adsorption with lymphocytes
| | |
3. Inactivation of IgM antibodies
| | | |
a. DTT treatment
| |
, b. Heat Inactivation
| |
4. Depletion of therapeutic anti-lymphocyte antibodies from serum
| | | | | | |
Why is calcium added to plasma to convert it to serum? - CORRECT
| | | | | | | | | | | | |
ANSWER✔✔-Calcium is added to the plasma to combine with the | | | | | | | | | |
citrate present in the anticoagulant.
| | | |
This restores a normal ionized calcium level and allows clotting to occur.
| | | | | | | | | | |
At what temperature should serum absorption with lymphocytes be
| | | | | | | | |
performed? - CORRECT ANSWER✔✔-Typically, auto-antibodies are cold
| | | | | | |
reacting and should be absorbed at 4oC while alloantibodies
| | | | | | | |
are warm reacting and should be absorbed at 37oC.
| | | | | | | |
DTT treatment is more specific than heat treatment in
| | | | | | | |
removing IgM antibodies. However, DTT can also affect the _______ in
| | | | | | | | | | |
IgG antibodies. - CORRECT ANSWER✔✔-disulfide bonds
| | | | |
What happens if we don't remove OKT3 prior to cytotoxicity assays? -
| | | | | | | | | | | |
CORRECT ANSWER✔✔-OKT3 is a monoclonal antibody directed against
| | | | | | | |
the CD3 molecule. Therefore, the
| | | |
binding of OKT3 to any CD3+ cell will activate complement and lyse the
| | | | | | | | | | | | |
target cell. This results
| | |
in falsely positive reactivity.
| | |
QUESTIONS WITH CORRECT ANSWERS
It is possible to convert the plasma
| | | | | |
into serum by recalcifying the plasma and allowing coagulation to occur.
| | | | | | | | | |
|- CORRECT ANSWER✔✔-Calcium is added to the plasma to form a
| | | | | | | | | | |
complex with the citrate present in the anti-coagulant. This restores a
| | | | | | | | | | |
normal
ionized calcium level allowing clotting to occur.
| | | | | |
Polyspecific antiserum |
can be______ with lymphocytes to determine if there is a single
| | | | | | | | | | |
antibody or multiple | |
antibodies. - CORRECT ANSWER✔✔-absorbed | | |
Another application of lymphocyte
| | |
absorption is to use the patient's own cells to remove_____. Absorption
| | | | | | | | | |
can be performed
| | |
at cold or warm temperatures depending on the type of antibody. -
| | | | | | | | | | | |
CORRECT ANSWER✔✔-autoantibody |
,_________ are typically | |
cold reacting (4oC) and________are typically warm reacting (22-37oC). -
| | | | | | | |
CORRECT ANSWER✔✔-Autoantibodies
| |
| B cell antibodies
| |
Patient serum may contain autoantibodies and/or IgM antibodies that
| | | | | | | | |
may interfere the binding of
| | | |
anti-HLA IgG antibodies to the antigen. In complement dependent
| | | | | | | | |
assays (CDC), IgM antibodies | | |
can activate complement; potentially leading to ___________. -
| | | | | | | |
CORRECT ANSWER✔✔-false positive reactivity.
| | |
DTT - CORRECT ANSWER✔✔-dithiothreitol, also known as Cleland's
| | | | | | | |
Reagent
DTT inactivates IgM molecules by cleaving the _________ in the
| | | | | | | | |
pentamer structure. - CORRECT ANSWER✔✔-disulphide bonds
| | | | |
Heat inactivation works by cleaving the disulphide bonds in the
| | | | | | | | |
pentamer structure, but is less specific than _____. - CORRECT
| | | | | | | | | |
ANSWER✔✔-DTT.
ALA - CORRECT ANSWER✔✔-therapeutic antilymphocyte
| | | |
,antibodies
Thymoglobulin - CORRECT ANSWER✔✔-purified, pasteurized, gamma| | | | | |
immune globulin, |
obtained by immunization of rabbits with human thymocytes
| | | | | | |
OKT3 - CORRECT ANSWER✔✔-a murine IgG monoclonal antibody
| | | | | | | |
directed against CD3 | |
If these therapeutic antilymphocyte
| | |
antibodies are present in the patient serum, they can cause_______
| | | | | | | | | |
reactivity in |
T cell cytotoxic assays. - CORRECT ANSWER✔✔-false positive
| | | | | | |
ALA removal is accomplished by absorption using _____coated with
| | | | | | | | |
anti-rabbit or anti-mouse immunoglobulin for Thymoblobulin or OKT3,
| | | | | | | |
respectively. - CORRECT ANSWER✔✔-magnetic beads
| | | |
serum preparations - CORRECT ANSWER✔✔-1. Recalcificiation of
| | | | | | |
plasma
2. Adsorption with lymphocytes
| | |
3. Inactivation of IgM antibodies
| | | |
a. DTT treatment
| |
, b. Heat Inactivation
| |
4. Depletion of therapeutic anti-lymphocyte antibodies from serum
| | | | | | |
Why is calcium added to plasma to convert it to serum? - CORRECT
| | | | | | | | | | | | |
ANSWER✔✔-Calcium is added to the plasma to combine with the | | | | | | | | | |
citrate present in the anticoagulant.
| | | |
This restores a normal ionized calcium level and allows clotting to occur.
| | | | | | | | | | |
At what temperature should serum absorption with lymphocytes be
| | | | | | | | |
performed? - CORRECT ANSWER✔✔-Typically, auto-antibodies are cold
| | | | | | |
reacting and should be absorbed at 4oC while alloantibodies
| | | | | | | |
are warm reacting and should be absorbed at 37oC.
| | | | | | | |
DTT treatment is more specific than heat treatment in
| | | | | | | |
removing IgM antibodies. However, DTT can also affect the _______ in
| | | | | | | | | | |
IgG antibodies. - CORRECT ANSWER✔✔-disulfide bonds
| | | | |
What happens if we don't remove OKT3 prior to cytotoxicity assays? -
| | | | | | | | | | | |
CORRECT ANSWER✔✔-OKT3 is a monoclonal antibody directed against
| | | | | | | |
the CD3 molecule. Therefore, the
| | | |
binding of OKT3 to any CD3+ cell will activate complement and lyse the
| | | | | | | | | | | | |
target cell. This results
| | |
in falsely positive reactivity.
| | |
