Portage Microbiology Exam #4 Questions
With Correct Answers
True or False: Growth media is best suited for distinguishing between
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two similar species of bacteria - CORRECT ANSWER✔✔-False. Growth
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media is designed to simply support (and not restrict) microbial growth
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What are the requirements of a fastidious microbe? (short answer) -
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CORRECT ANSWER✔✔-A fastidious microbe is an organism with
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complex growth requirements such that is absent it will not grow.
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Enriched medias thus contain these specific and essential nutrients
| | | | | | | | |
required for the growth of a particular subset of microorganisms.
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True or False: LB agar is classified as a selective, non-differential media -
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CORRECT ANSWER✔✔-False |
What is agar used for in microbiology? (short answer) - CORRECT
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ANSWER✔✔-Agar is used to create a solid, smooth surface on which | | | | | | | | | | |
microbes can grow | |
Alpha hemolysis (matching) - CORRECT ANSWER✔✔-Incomplete
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hemolytic activity |
, Beta hemolysis (matching) - CORRECT ANSWER✔✔-Complete hemolytic
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activity
|
Gamma hemolysis (matching) - CORRECT ANSWER✔✔-No hemolytic
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activity
Columbia CNA agar is most closely related to which media (multiple
| | | | | | | | | | |
choice) - CORRECT ANSWER✔✔-CNA agar is similar to VAP as it is also
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enriched with blood and allows for differentiation based on hemolytic
| | | | | | | | | |
patterns
True or False: Chocolate agar gets its brown color from cocoa to produce
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an enriched media - CORRECT ANSWER✔✔-False
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In an attempt to detect the presence of the pathogenic strain of e coli
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O157:H7, a researcher spread culture onto a MacConkey agar with
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failed results. What type of agar should they (correctly) try next? Why?
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(short answer) - CORRECT ANSWER✔✔-The microbe should be plated
| | | | | | | | |
on SMAC as it is specifically formulated to detect O157:H7. Pathogenic e
| | | | | | | | | | |
|coli cannot ferment sorbitol while non-pathogenic e coli can ferment
| | | | | | | | | |
both sorbitol and lactose. Therefore, colonies that ferment can be
| | | | | | | | | |
differentiated from non-fermenters. | |
With Correct Answers
True or False: Growth media is best suited for distinguishing between
| | | | | | | | | | |
two similar species of bacteria - CORRECT ANSWER✔✔-False. Growth
| | | | | | | | |
media is designed to simply support (and not restrict) microbial growth
| | | | | | | | | |
What are the requirements of a fastidious microbe? (short answer) -
| | | | | | | | | | |
CORRECT ANSWER✔✔-A fastidious microbe is an organism with
| | | | | | | |
complex growth requirements such that is absent it will not grow.
| | | | | | | | | | |
Enriched medias thus contain these specific and essential nutrients
| | | | | | | | |
required for the growth of a particular subset of microorganisms.
| | | | | | | | |
True or False: LB agar is classified as a selective, non-differential media -
| | | | | | | | | | | | |
CORRECT ANSWER✔✔-False |
What is agar used for in microbiology? (short answer) - CORRECT
| | | | | | | | | | |
ANSWER✔✔-Agar is used to create a solid, smooth surface on which | | | | | | | | | | |
microbes can grow | |
Alpha hemolysis (matching) - CORRECT ANSWER✔✔-Incomplete
| | | | | |
hemolytic activity |
, Beta hemolysis (matching) - CORRECT ANSWER✔✔-Complete hemolytic
| | | | | |
activity
|
Gamma hemolysis (matching) - CORRECT ANSWER✔✔-No hemolytic
| | | | | | |
activity
Columbia CNA agar is most closely related to which media (multiple
| | | | | | | | | | |
choice) - CORRECT ANSWER✔✔-CNA agar is similar to VAP as it is also
| | | | | | | | | | | | |
enriched with blood and allows for differentiation based on hemolytic
| | | | | | | | | |
patterns
True or False: Chocolate agar gets its brown color from cocoa to produce
| | | | | | | | | | | |
an enriched media - CORRECT ANSWER✔✔-False
| | | | | |
In an attempt to detect the presence of the pathogenic strain of e coli
| | | | | | | | | | | | | |
O157:H7, a researcher spread culture onto a MacConkey agar with
| | | | | | | | | |
failed results. What type of agar should they (correctly) try next? Why?
| | | | | | | | | | | |
(short answer) - CORRECT ANSWER✔✔-The microbe should be plated
| | | | | | | | |
on SMAC as it is specifically formulated to detect O157:H7. Pathogenic e
| | | | | | | | | | |
|coli cannot ferment sorbitol while non-pathogenic e coli can ferment
| | | | | | | | | |
both sorbitol and lactose. Therefore, colonies that ferment can be
| | | | | | | | | |
differentiated from non-fermenters. | |
