Portage Microbiology Exam #3 Questions
With Correct Answers
What is the total magnification of a sample imaged with a 60x objective
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and a 10x eyepiece? (short answer) - CORRECT ANSWER✔✔-60 x 10 =
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600x magnification
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True or False: A cell that is adherent, flat, and unstained is easily
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identified using bright field microscopy - CORRECT ANSWER✔✔-False.
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Adherent, flat cells are almost invisible due to the limits on both
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resolution and contrast | |
Which of the following could be seen clearly by the unaided eye?
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(multiple choice) - CORRECT ANSWER✔✔-Protozoa with diameter of
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150um and skin cell with diameter of 1500 um (have to be greater than
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100um
Label the following unmarked microscope components by matching it
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with the components provided - CORRECT ANSWER✔✔-1. Eyepiece
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2. Neck
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3. Fine adjustment knob
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4. Objective
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, 5. Stage
|
6. Base
|
This type of microscope is best suited for visualizing GFP, RFP and YFP
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proteins (given list of microscopes) - CORRECT ANSWER✔✔-
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Flourescence
This type of microscope can provide detailed images of live cells without
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staining (given list of microscopes) - CORRECT ANSWER✔✔-Phase-
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Contrast
This type of microscope is used to greatly increase the contrast between
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|samples and background by reflecting light off of the specimen (given
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list of microscopes) - CORRECT ANSWER✔✔-Dark Field
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This type of microscope is capable of capturing images in multiple focal
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planes rendering specimen in 3D (given list of microscopes) - CORRECT
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ANSWER✔✔-Confocal
Identify what type of electron microscope was used to capture the
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following image and explain your choice - CORRECT ANSWER✔✔-A:
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TEM, shows 2-D rendering
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B: SEM shows 3-D shell models
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With Correct Answers
What is the total magnification of a sample imaged with a 60x objective
| | | | | | | | | | | | |
and a 10x eyepiece? (short answer) - CORRECT ANSWER✔✔-60 x 10 =
| | | | | | | | | | | |
600x magnification
|
True or False: A cell that is adherent, flat, and unstained is easily
| | | | | | | | | | | | |
identified using bright field microscopy - CORRECT ANSWER✔✔-False.
| | | | | | | |
Adherent, flat cells are almost invisible due to the limits on both
| | | | | | | | | | | |
resolution and contrast | |
Which of the following could be seen clearly by the unaided eye?
| | | | | | | | | | | |
(multiple choice) - CORRECT ANSWER✔✔-Protozoa with diameter of
| | | | | | | |
150um and skin cell with diameter of 1500 um (have to be greater than
| | | | | | | | | | | | | |
100um
Label the following unmarked microscope components by matching it
| | | | | | | | |
with the components provided - CORRECT ANSWER✔✔-1. Eyepiece
| | | | | | |
2. Neck
|
3. Fine adjustment knob
| | |
4. Objective
|
, 5. Stage
|
6. Base
|
This type of microscope is best suited for visualizing GFP, RFP and YFP
| | | | | | | | | | | | |
proteins (given list of microscopes) - CORRECT ANSWER✔✔-
| | | | | | |
Flourescence
This type of microscope can provide detailed images of live cells without
| | | | | | | | | | |
staining (given list of microscopes) - CORRECT ANSWER✔✔-Phase-
| | | | | | | |
Contrast
This type of microscope is used to greatly increase the contrast between
| | | | | | | | | | |
|samples and background by reflecting light off of the specimen (given
| | | | | | | | | | |
list of microscopes) - CORRECT ANSWER✔✔-Dark Field
| | | | | |
This type of microscope is capable of capturing images in multiple focal
| | | | | | | | | | | |
planes rendering specimen in 3D (given list of microscopes) - CORRECT
| | | | | | | | | | |
ANSWER✔✔-Confocal
Identify what type of electron microscope was used to capture the
| | | | | | | | | | |
following image and explain your choice - CORRECT ANSWER✔✔-A:
| | | | | | | | |
TEM, shows 2-D rendering
| | |
B: SEM shows 3-D shell models
| | | | |
