DAY 8 & 9 FECAL ANALYSIS / PERIANAL SWAB
Clinical Internship – CM
PERIANAL SWAB
PRINCIPLE:
• The clear-cellulose tape preparation is the MOST 2. Press the tape firmly several times against the right and
WIDELY USED PROCEDURE for the detection of left perianal folds.
human pinworm infections 3. Smooth the tape back on a clean glass microscope
slide, adhesive side down.
4. Label the glass slide with patient’s name, birthday, initial
of collector, and date and time of collection.
5. Examine the slide under a microscope using a low
power (10x) objective.
E. vermicularis eggs at LPO
Enterobius vermicularis egg
E. vermicularis eggs at LPO
Enterobius vermicularis adult worm (pinworm on the
perianal skin)
AREA AND TIME OF SPECIMEN COLLECTION
• Area: Perianal skin
• Time: EARLY-MORNING SAMPLE (before the patient
has bathed or used the toilet)
NOTE:
• Up to six consecutive day morning samples
should be collected before a negative result is
issued.
MATERIALS
Comparison of E. vermicularis in different microscopic
• PPE identification technique
• Tongue depressor
• Scotch tape
• Glass slide REPORTING
• Microscope
• Report the organism and stage
o Example:
PROCEDURE ▪ Enterobius vermicularis eggs present
SCOTCH TAPE TEST PROCEDURE: ▪ Enterobius vermicularis adult worm
1. Fold the end of a 10 cm piece of transparent tape, present
adhesive out, over the end of a tongue depressor.
CI | First Semester 1
, DAY 8 & 9 FECAL ANALYSIS / PERIANAL SWAB
Clinical Internship – CM
FECAL ANALYSIS
Basic Guidelines in Stool Analysis
1. Do not eat, drink, smoke, apply cosmetics or manipulate
contact lenses in work area.
2. Adopt universal precautions as well as standard
microbiological laboratory practices (BIOSAFETY
LEVEL 2)
3. Wear protective safety glasses, gloves, and laboratory
coat when processing specimens.
4. Use biological safety cabinets as needed
5. If you have cuts or abrasions on the skin of your hands,
cover them with adhesive dressing.
6. If you use any sharp instruments, dispose of them in a
“sharps” container for decontamination.
7. Remove gloves and wash your hands after completing • Amebiasis / Intestinal giardiasis – recommend at
any task involving the handling of fecal material. least 3 stools (every other day)
8. Decontaminate work surface at least once a day and • DIRECT WET MOUNT
after any spill of potentially infectious material. • FECAL CONCENTRATION TECHNIQUE
• PERMANENT STAINED SMEAR
SPECIMEN COLLECTION, TRANSPORT, AND PRESERVATION 2. Specimens must be collected before the start of
PROCEDURES treatment regimens or collection must be delayed until
1. Collect the stool in a dry, clean, leakproof container. after the effects have passed because it may result to
Make sure no urine, water, soil, or other material gets in suppression of parasites.
the container. 3. Such substances includes: antacids, kaolin, mineral oil,
o Stool sample should be collected on a clean and other oily materials, non-absorbable anti-diarrheal
cardboard or a pan. Transport to a clean, dry, preparations, barium or bismuth (7-10 days needed for
sterile, wide-mouthed container with a tight- clearance of effects), antimicrobial agents (2-3
fitting lid with sufficient moisture before weeks), and gallbladder dyes (3 weeks).
submitting to the laboratory. o Specimen collection may need to be repeated
o Never contaminate with urine and water. if the first examination is negative. If possible,
3 specimens passed at intervals of 2-3 days
Possible stool contaminants: should be examined.
4. Fresh stool should be examined, processed, or
• Urine – know to destroy some parasites
preserved immediately. An exception is specimens kept
• Toilet bowl transfer – free-living protozoa and under refrigeration when preservatives are not
nematodes may be confused with human parasites. available; these specimens are suitable for antigen
• Water – destroy select parasite, such as schistosome testing only.
eggs and amebic trophozoites. 5. Fresh stool samples should be delivered and processed
• Toilet paper – mask parasites or make examination of into the laboratory:
the sample difficult. o Liquid stool – within 30 minutes
o Semisolid stool – within 1 hour
2. Container should be properly labeled at the side (body) o Formed stool – within 1 hour
of the container (not on the lid) with necessary
information • Preserved with fixative solution if there will be a delay
o Complete name in processing.
o Date and time of collection
GENERAL CONSIDERATIONS FOR PROPER SPECIMEN 6. Trophozoite: WATERY STOOL
COLLECTION, TRANSPORT, AND PRESERVATION Cyst: FORMED STOOL (easier to be identified)
PROCEDURES
1. Stool sample can be passed and collected on a
sufficient amounts so repetition of examination can be
done if necessary.
o 2-5 grams
o Pea / walnut / thumb-sized
NOTE:
• The image demonstrates the distribution if protozoa
in relation to stool consistency and should be taken
into consideration when specimens are received.
CI | First Semester 2
Clinical Internship – CM
PERIANAL SWAB
PRINCIPLE:
• The clear-cellulose tape preparation is the MOST 2. Press the tape firmly several times against the right and
WIDELY USED PROCEDURE for the detection of left perianal folds.
human pinworm infections 3. Smooth the tape back on a clean glass microscope
slide, adhesive side down.
4. Label the glass slide with patient’s name, birthday, initial
of collector, and date and time of collection.
5. Examine the slide under a microscope using a low
power (10x) objective.
E. vermicularis eggs at LPO
Enterobius vermicularis egg
E. vermicularis eggs at LPO
Enterobius vermicularis adult worm (pinworm on the
perianal skin)
AREA AND TIME OF SPECIMEN COLLECTION
• Area: Perianal skin
• Time: EARLY-MORNING SAMPLE (before the patient
has bathed or used the toilet)
NOTE:
• Up to six consecutive day morning samples
should be collected before a negative result is
issued.
MATERIALS
Comparison of E. vermicularis in different microscopic
• PPE identification technique
• Tongue depressor
• Scotch tape
• Glass slide REPORTING
• Microscope
• Report the organism and stage
o Example:
PROCEDURE ▪ Enterobius vermicularis eggs present
SCOTCH TAPE TEST PROCEDURE: ▪ Enterobius vermicularis adult worm
1. Fold the end of a 10 cm piece of transparent tape, present
adhesive out, over the end of a tongue depressor.
CI | First Semester 1
, DAY 8 & 9 FECAL ANALYSIS / PERIANAL SWAB
Clinical Internship – CM
FECAL ANALYSIS
Basic Guidelines in Stool Analysis
1. Do not eat, drink, smoke, apply cosmetics or manipulate
contact lenses in work area.
2. Adopt universal precautions as well as standard
microbiological laboratory practices (BIOSAFETY
LEVEL 2)
3. Wear protective safety glasses, gloves, and laboratory
coat when processing specimens.
4. Use biological safety cabinets as needed
5. If you have cuts or abrasions on the skin of your hands,
cover them with adhesive dressing.
6. If you use any sharp instruments, dispose of them in a
“sharps” container for decontamination.
7. Remove gloves and wash your hands after completing • Amebiasis / Intestinal giardiasis – recommend at
any task involving the handling of fecal material. least 3 stools (every other day)
8. Decontaminate work surface at least once a day and • DIRECT WET MOUNT
after any spill of potentially infectious material. • FECAL CONCENTRATION TECHNIQUE
• PERMANENT STAINED SMEAR
SPECIMEN COLLECTION, TRANSPORT, AND PRESERVATION 2. Specimens must be collected before the start of
PROCEDURES treatment regimens or collection must be delayed until
1. Collect the stool in a dry, clean, leakproof container. after the effects have passed because it may result to
Make sure no urine, water, soil, or other material gets in suppression of parasites.
the container. 3. Such substances includes: antacids, kaolin, mineral oil,
o Stool sample should be collected on a clean and other oily materials, non-absorbable anti-diarrheal
cardboard or a pan. Transport to a clean, dry, preparations, barium or bismuth (7-10 days needed for
sterile, wide-mouthed container with a tight- clearance of effects), antimicrobial agents (2-3
fitting lid with sufficient moisture before weeks), and gallbladder dyes (3 weeks).
submitting to the laboratory. o Specimen collection may need to be repeated
o Never contaminate with urine and water. if the first examination is negative. If possible,
3 specimens passed at intervals of 2-3 days
Possible stool contaminants: should be examined.
4. Fresh stool should be examined, processed, or
• Urine – know to destroy some parasites
preserved immediately. An exception is specimens kept
• Toilet bowl transfer – free-living protozoa and under refrigeration when preservatives are not
nematodes may be confused with human parasites. available; these specimens are suitable for antigen
• Water – destroy select parasite, such as schistosome testing only.
eggs and amebic trophozoites. 5. Fresh stool samples should be delivered and processed
• Toilet paper – mask parasites or make examination of into the laboratory:
the sample difficult. o Liquid stool – within 30 minutes
o Semisolid stool – within 1 hour
2. Container should be properly labeled at the side (body) o Formed stool – within 1 hour
of the container (not on the lid) with necessary
information • Preserved with fixative solution if there will be a delay
o Complete name in processing.
o Date and time of collection
GENERAL CONSIDERATIONS FOR PROPER SPECIMEN 6. Trophozoite: WATERY STOOL
COLLECTION, TRANSPORT, AND PRESERVATION Cyst: FORMED STOOL (easier to be identified)
PROCEDURES
1. Stool sample can be passed and collected on a
sufficient amounts so repetition of examination can be
done if necessary.
o 2-5 grams
o Pea / walnut / thumb-sized
NOTE:
• The image demonstrates the distribution if protozoa
in relation to stool consistency and should be taken
into consideration when specimens are received.
CI | First Semester 2
