Clinical Microscopy - Concentration Technique & Fecal Occult Blood

DAY 10 & 11 CONCENTRATION TECHNIQUE / FECAL OCCULT BLOOD
Clinical Internship – CLINICAL MICROSCOPY


FECAL CONCENTRATION TECHNIQUE




SEDIMENTATION TECHNIQUE
• Formalin-ethyl acetate technique
• Use solutions of lower specific gravity than the
parasitic organisms, thus concentrating the latter in the
sediment

Formalin-Ethyl Acetate Concentration Technique
MATERIALS: Parasites Commonly Seen in Formalin-Ethyl Acetate
• Vial Concentration Technique
• Gauze
• 10% formalin
• Ether (Ethyl Acetate)
• Centrifuge tubes (15ml)
• Small beaker 25, 50, or 100ml
• 0.5% NSS
• Applicator sticks
• Rubber stopper or cork
• Slides

Procedures:




ADVANTAGES (Formalin-Ethyl Acetate Technique)
1. Maximizes the numbers of organisms detected which
may be too scanty to be seen by direct microscopy
alone.
2. Separate the parasites from fecal debris.
3. Recovers most ova, cysts, and larvae
4. Morphology of most parasites is retained



CI | First Semester 1