MED 194 | Biochemistry Laboratory
2nd term (A.Y. 2020-2021)
LEYNEIL MARCELINO 2nd year BSMT/MLS
Laboratory Safety and Basic Equipment used in
Biochemistry Laboratory 8. Never taste or smell the chemicals.
9. On-going experiments should never be left
Learning Outcomes
unattended.
At the end of the exercise, the learner should be able to: 10. Dispose wastes properly
Protective laboratory safety while working in BIOCHEMISTRY LABORATORY APPARATUS
the laboratory AND EQUIPMENT
Biochemistry laboratory requires several apparatus
Identify the precautionary measures that must and equipment to be used in the different experiments. It is
be taken to prevent contamination. important that students know their use and proper care and
Discuss the proper use of the different handling, not only for their own safety but also for the
equipment in the laboratory. safety of the personnel around them. Provided below is the
list of different glasswares, equipment, and apparatus
SAFETY PRECAUTIONS IN THE LABORATORY
commonly used in the laboratory.
The laboratory gown is required to be worn at
1. Alcohol lamp 11. Test tube, rack, holder,
all times while inside the laboratory to serve as brush
a primary layer of protection against chemical 2. Aspiratory bulb 12. Tripod
spills. Its length must extend below the knee and 3. Beakers 13. Wire gauze
preferably, should have long sleeves. It must be 4. Bunsen burner 14. Analytical balance
easy to remove in case of an emergency. 5. Clay triangle 15. Water bath
Closed-toe shoes must be worn. 6. Erlenmeyer flasks 16. Fume hood
Long hair must be tied. 7. Funnel 17. UV VIS
spectrophotometer
Loose jewelry pieces or accessories worn must
8. Graduated Cylinders 18. pH meter
be removed. 9. Hot plates 19. Centrifuge
10. Pipettes 20. Cuvettes
RULES ON LABORATORY SAFETY Pipette Classification
1. During the experiment, all bags, books and other I. Design
materials must be placed in the bag racks. No
unnecessary items should be found in the To contain (TC)
working area. To deliver (TD)
2. Turn off/switched to silent mode all the
electronic devices inside the laboratory room, II. Drainage characteristics
unless for documentation purposes.
3. Eating and drinking are strictly prohibited inside Blowout
the laboratory room. Self-draining
4. The experiment should be studied before coming
ACTIVITY #2 MACROMOLECULES
to class. Be aware of the precautions about the
procedure and the reagents to be used. Introduction
5. Be familiar with the location of safety equipment
and the first aid cabinet. Biochemistry is study of the chemical substances
6. Report any accidents (even minor ceases) or found in living organisms & the chemical interactions of
situations that may lead to an accident to the these substances with each other. It is the science concerned
laboratory instructor and laboratory technician. with the chemical basis of life.
7. Do not perform any unauthorized experiment.
Bioinorganic substances do not contain carbon
Aside from posing a risk of accidents, it will also
eg. water. Bioorganic substances contain carbon.
be a waste of time and chemicals.
, All living things are made up of four classes of
large biological molecules or also called as macromolecules:
Carbohydrates, Lipids, Proteins and Nucleic acids which are
Bioorganic substances. Macromolecules are large molecules
composed of thousands of covalently connected atoms.
MED 194 | Biochemistry Laboratory
2nd term (A.Y. 2020-2021)
LEYNEIL MARCELINO 2nd year BSMT/MLS
WATER
Water is important substance inside our body.
It is the most abundant bio inorganic substance.
In human body it composed 70% of water in our
total body mass.
Water Molecule
Bio – Inorganic substance
Irregular, slightly skewed tetrahedron with oxygen
at its center.
IUPAC name for H20 (oxidane)
Is the amount of heat that must be absorb or loss
Properties of Water to change its temperature by 1°c since water has a
high specific heat it can absorb large amount of
Polar molecule
heat energy before it begins to get hot.
Cohesion – relation between water molecule to
Water releases heat energy slowly.
another molecule
Adhesion – water to other substances
High specific heat High heat of vaporization
High heat of vaporization Because of hydrogen bonding water absorbs large
Medium for chemical Reactions amount of heat as it vaporizes.
Water releases large amounts of heat as it
condenses.
Medium for Chemical reactions
It is the Universal Solvent.
Water
40 % - 75% average water content of the human
body. (depends on the age)
Intracellular Fluid (ICF) – two – thirds 2/3 of
Cohesion – relation between water molecule to another total water our body composition.(Inside the cell)
molecule.
Extracellular Fluid (ECF) – one third – outside
Adhesion – water to other substances. of the cell.
o Intravascular ECF – plasma – fluid
portion of the blood
2nd term (A.Y. 2020-2021)
LEYNEIL MARCELINO 2nd year BSMT/MLS
Laboratory Safety and Basic Equipment used in
Biochemistry Laboratory 8. Never taste or smell the chemicals.
9. On-going experiments should never be left
Learning Outcomes
unattended.
At the end of the exercise, the learner should be able to: 10. Dispose wastes properly
Protective laboratory safety while working in BIOCHEMISTRY LABORATORY APPARATUS
the laboratory AND EQUIPMENT
Biochemistry laboratory requires several apparatus
Identify the precautionary measures that must and equipment to be used in the different experiments. It is
be taken to prevent contamination. important that students know their use and proper care and
Discuss the proper use of the different handling, not only for their own safety but also for the
equipment in the laboratory. safety of the personnel around them. Provided below is the
list of different glasswares, equipment, and apparatus
SAFETY PRECAUTIONS IN THE LABORATORY
commonly used in the laboratory.
The laboratory gown is required to be worn at
1. Alcohol lamp 11. Test tube, rack, holder,
all times while inside the laboratory to serve as brush
a primary layer of protection against chemical 2. Aspiratory bulb 12. Tripod
spills. Its length must extend below the knee and 3. Beakers 13. Wire gauze
preferably, should have long sleeves. It must be 4. Bunsen burner 14. Analytical balance
easy to remove in case of an emergency. 5. Clay triangle 15. Water bath
Closed-toe shoes must be worn. 6. Erlenmeyer flasks 16. Fume hood
Long hair must be tied. 7. Funnel 17. UV VIS
spectrophotometer
Loose jewelry pieces or accessories worn must
8. Graduated Cylinders 18. pH meter
be removed. 9. Hot plates 19. Centrifuge
10. Pipettes 20. Cuvettes
RULES ON LABORATORY SAFETY Pipette Classification
1. During the experiment, all bags, books and other I. Design
materials must be placed in the bag racks. No
unnecessary items should be found in the To contain (TC)
working area. To deliver (TD)
2. Turn off/switched to silent mode all the
electronic devices inside the laboratory room, II. Drainage characteristics
unless for documentation purposes.
3. Eating and drinking are strictly prohibited inside Blowout
the laboratory room. Self-draining
4. The experiment should be studied before coming
ACTIVITY #2 MACROMOLECULES
to class. Be aware of the precautions about the
procedure and the reagents to be used. Introduction
5. Be familiar with the location of safety equipment
and the first aid cabinet. Biochemistry is study of the chemical substances
6. Report any accidents (even minor ceases) or found in living organisms & the chemical interactions of
situations that may lead to an accident to the these substances with each other. It is the science concerned
laboratory instructor and laboratory technician. with the chemical basis of life.
7. Do not perform any unauthorized experiment.
Bioinorganic substances do not contain carbon
Aside from posing a risk of accidents, it will also
eg. water. Bioorganic substances contain carbon.
be a waste of time and chemicals.
, All living things are made up of four classes of
large biological molecules or also called as macromolecules:
Carbohydrates, Lipids, Proteins and Nucleic acids which are
Bioorganic substances. Macromolecules are large molecules
composed of thousands of covalently connected atoms.
MED 194 | Biochemistry Laboratory
2nd term (A.Y. 2020-2021)
LEYNEIL MARCELINO 2nd year BSMT/MLS
WATER
Water is important substance inside our body.
It is the most abundant bio inorganic substance.
In human body it composed 70% of water in our
total body mass.
Water Molecule
Bio – Inorganic substance
Irregular, slightly skewed tetrahedron with oxygen
at its center.
IUPAC name for H20 (oxidane)
Is the amount of heat that must be absorb or loss
Properties of Water to change its temperature by 1°c since water has a
high specific heat it can absorb large amount of
Polar molecule
heat energy before it begins to get hot.
Cohesion – relation between water molecule to
Water releases heat energy slowly.
another molecule
Adhesion – water to other substances
High specific heat High heat of vaporization
High heat of vaporization Because of hydrogen bonding water absorbs large
Medium for chemical Reactions amount of heat as it vaporizes.
Water releases large amounts of heat as it
condenses.
Medium for Chemical reactions
It is the Universal Solvent.
Water
40 % - 75% average water content of the human
body. (depends on the age)
Intracellular Fluid (ICF) – two – thirds 2/3 of
Cohesion – relation between water molecule to another total water our body composition.(Inside the cell)
molecule.
Extracellular Fluid (ECF) – one third – outside
Adhesion – water to other substances. of the cell.
o Intravascular ECF – plasma – fluid
portion of the blood
