v.3
Exercise 6: Questions
Perm:
Table: Testing the Model, p.117
The model of GA and ABA action summarized previously makes predictions about the effect of these
hormones on alpha-amylase production by the aleurone layer. In this section of the exercise, we will test
these predictions to see if the model is accurate.
Complete We’ll begin by tabulating the predictions of the model. Using the model, predict the level of amylase you
Before would expect to find (zero, low, medium, high) in cereal grains treated as shown in the chart below.
Lab
Treatment Amylase Produced
No Hormone
GA (10 μM)
ABA (10 μM)
GA (10 μM) + ABA (10 μM)
Table: Hormone Treatment, p.119
The incubation medium to which you will add your half-seeds will contain hormones and ions that would
be supplied by the embryo if it were present.
Complete 1. Calculate the volume of each component (with units) that you will need to add to each plate to
Before obtain a final volume of 3 mL. Enter those values in the following table.
Lab
Stock CaCl2 Stock Chloramphenicol Stock GA Stock ABA
Treatment
(10 mM)* (10 mg/mL) (10 mM) (10 mM)
1 No Hormone 2.997 ml 3 ul
2 GA (10 μM)
3 ABA (10 μM)
GA (10 μM) +
4
ABA (10 μM)
, Figure: Cellulose Acetate Plate, p.124
Below is an example of a cellulose acetate plate. Use this figure to answer Q1 and Q2.
Q1. How many bands of activity do you see?
Complete
Before
Lab
Q2. Do all of them change with treatment in the same way? Explain.
Complete
During
Lab
Exercise 6: Questions
Perm:
Table: Testing the Model, p.117
The model of GA and ABA action summarized previously makes predictions about the effect of these
hormones on alpha-amylase production by the aleurone layer. In this section of the exercise, we will test
these predictions to see if the model is accurate.
Complete We’ll begin by tabulating the predictions of the model. Using the model, predict the level of amylase you
Before would expect to find (zero, low, medium, high) in cereal grains treated as shown in the chart below.
Lab
Treatment Amylase Produced
No Hormone
GA (10 μM)
ABA (10 μM)
GA (10 μM) + ABA (10 μM)
Table: Hormone Treatment, p.119
The incubation medium to which you will add your half-seeds will contain hormones and ions that would
be supplied by the embryo if it were present.
Complete 1. Calculate the volume of each component (with units) that you will need to add to each plate to
Before obtain a final volume of 3 mL. Enter those values in the following table.
Lab
Stock CaCl2 Stock Chloramphenicol Stock GA Stock ABA
Treatment
(10 mM)* (10 mg/mL) (10 mM) (10 mM)
1 No Hormone 2.997 ml 3 ul
2 GA (10 μM)
3 ABA (10 μM)
GA (10 μM) +
4
ABA (10 μM)
, Figure: Cellulose Acetate Plate, p.124
Below is an example of a cellulose acetate plate. Use this figure to answer Q1 and Q2.
Q1. How many bands of activity do you see?
Complete
Before
Lab
Q2. Do all of them change with treatment in the same way? Explain.
Complete
During
Lab
