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±
, 2.I → MICROSCOPY
" ☒ 1
11 1
Magnification
-
• the number of times that the
€000•
mm µ rn
image
size
of an
object is
bigger than the actual
µm €000m
nm
of the
object
Resolution → the
ability to distinguish two iniiiaijiiñéiisiiié
=
.
points É % 77
close
being se prate
as . i. ii. . " i
"
Magnification imagesize
=
object size
① To calculate
i] Measure the
magnification of diagram
the a :
of the real object in
the actual
length ,
this is mm ,
length
ii] Measure the of the image /diagram in
length .
mm . this is the
image length
iii]
Magnification =
image.org#
actual
length
6km
② To calculate the of the image scale bar
magnification using a : t-1
i] Measure the of the scale bar and convert it to micrometers ( mm item)
ii]
length
Magnification scatebarmeasurement
=
actual
length
TYPES OF MICROSCOPY
① Light microscope
1) Uses lenses to focus
rays of light onto the
2) Lowest resolution ( 0.2µm) specimen
3) Maximum
magnification ✗ 1500
② Electron
1) Usesmicroscopy of electrons
a beam
2) shorter therefore resolution
wavelength
3) Resolution 0.2 nm -
•
,
greater
4) Maximum 500000
magnification
✗
A) Transmission electron microscope ITEM]
the
i] Use to focus a beam of electrons which passes
electromagnets
ii. Specimen is stained with metals (lead /osmium
specimen ,
through
iii. Some parts take heavy large charged ions ,
positively .
more ions so are more dense
up .
±
, 2.I → MICROSCOPY
" ☒ 1
11 1
Magnification
-
• the number of times that the
€000•
mm µ rn
image
size
of an
object is
bigger than the actual
µm €000m
nm
of the
object
Resolution → the
ability to distinguish two iniiiaijiiñéiisiiié
=
.
points É % 77
close
being se prate
as . i. ii. . " i
"
Magnification imagesize
=
object size
① To calculate
i] Measure the
magnification of diagram
the a :
of the real object in
the actual
length ,
this is mm ,
length
ii] Measure the of the image /diagram in
length .
mm . this is the
image length
iii]
Magnification =
image.org#
actual
length
6km
② To calculate the of the image scale bar
magnification using a : t-1
i] Measure the of the scale bar and convert it to micrometers ( mm item)
ii]
length
Magnification scatebarmeasurement
=
actual
length
TYPES OF MICROSCOPY
① Light microscope
1) Uses lenses to focus
rays of light onto the
2) Lowest resolution ( 0.2µm) specimen
3) Maximum
magnification ✗ 1500
② Electron
1) Usesmicroscopy of electrons
a beam
2) shorter therefore resolution
wavelength
3) Resolution 0.2 nm -
•
,
greater
4) Maximum 500000
magnification
✗
A) Transmission electron microscope ITEM]
the
i] Use to focus a beam of electrons which passes
electromagnets
ii. Specimen is stained with metals (lead /osmium
specimen ,
through
iii. Some parts take heavy large charged ions ,
positively .
more ions so are more dense
up .
