Lab_Notebook_7

Lab Notebook PLO7 Secondary Assays for Bacterial Characterization Objective: To profile bacterial populations based on additional biochemical approaches to better determine growth/metabolic characteristics. Indole Test: Biochemical test to determine a bacterial species ability to convert (breakdown) tryptophan into indole. Procedure: Test #1 1. Using a sterile loop pick an isolated colony from BAP 2. Smear directly onto the reaction area of the slide. 3. Examine test area for color change within 20 seconds Note: Indole negative= yellow color Indole positive = Red/Pink orange color Test #2 1. Using a sterile loop pick an isolated colony from BAP 2. Inoculate bacteria in media broth 3. Grow at 37C overnight 4. Add ~ 5 drops of Kovac’s reagent to culture Note: Indole negative = Yellow