Title : Cloning Vector
Index
1.Examples:-Plasmid & Bacteriophase
Use of plasmid in R-DNA technology
pBR322 Sites
pBR322 Restriction Sites
2.Characteristics of a cloning Vectors
Origin of Replication
3.Selection of Recombinants Non Recombinants
Inactivation Of Antibiotic Resistant gene
Insertion Inactivation of β-galactosidase gene
4.Vectors for cloning gene in Plant and animals
, Page:
Date: /
CLONING V£CTORS
Vectaua UAed ocloning th
alcsiMedgehe
O make _mahy Copies _duined gehe) H
Callhd cloning Vectons
ExamplA 1 - PlaAmid
Bacteiophagus
PlaAmid
plaAmid A the extHachhomDAomal
NA Bactenia
1 LA DoublAHandd ondinculaH DNA
E The
TheReplicaHan plaAmidDNA Aindependeht
maUn maun DNA bactexia
UAA 0blasmid_in R:DNA tchnalogy
UAd a Cloning VecHanA If we 4ain
the Plaamid DXA uwlh the duined genc
then we Cah mulhply th_daiHed gene(cloning
lu'ththe plau mid 0h we ah faim mahy
Capis duuiMed gne
UAed as Genchic VecknNA plasmidQL Aed
A
GenchcVectaya becauu IE A UAedoy
THansfen the deiued ho m mi (HgahlAmo
Qnothek HganismPlaamid Cah be eouily
takenQut om bachaial ClL Can be
bacteulal Cell)
he indenkd into _difunent
Index
1.Examples:-Plasmid & Bacteriophase
Use of plasmid in R-DNA technology
pBR322 Sites
pBR322 Restriction Sites
2.Characteristics of a cloning Vectors
Origin of Replication
3.Selection of Recombinants Non Recombinants
Inactivation Of Antibiotic Resistant gene
Insertion Inactivation of β-galactosidase gene
4.Vectors for cloning gene in Plant and animals
, Page:
Date: /
CLONING V£CTORS
Vectaua UAed ocloning th
alcsiMedgehe
O make _mahy Copies _duined gehe) H
Callhd cloning Vectons
ExamplA 1 - PlaAmid
Bacteiophagus
PlaAmid
plaAmid A the extHachhomDAomal
NA Bactenia
1 LA DoublAHandd ondinculaH DNA
E The
TheReplicaHan plaAmidDNA Aindependeht
maUn maun DNA bactexia
UAA 0blasmid_in R:DNA tchnalogy
UAd a Cloning VecHanA If we 4ain
the Plaamid DXA uwlh the duined genc
then we Cah mulhply th_daiHed gene(cloning
lu'ththe plau mid 0h we ah faim mahy
Capis duuiMed gne
UAed as Genchic VecknNA plasmidQL Aed
A
GenchcVectaya becauu IE A UAedoy
THansfen the deiued ho m mi (HgahlAmo
Qnothek HganismPlaamid Cah be eouily
takenQut om bachaial ClL Can be
bacteulal Cell)
he indenkd into _difunent
