Biochemistry C 785 Readiness Check 1 2020 – Western Governors University

Biochemistry Readiness Check The 2nd OA Readiness Check is designed to give you additional practice with new assessment-style questions to help prepare for success on the OA. Thus, please try to mimic exam-like conditions by taking the test in one 2 hr sitting without any study aids apart from your whiteboard/scrap paper. Note: Responses cannot be edited. You will be able to see the results and feedback after submitting the test. A score of 70% is required for OA approval. If you score below 70%, please speak with a Course Instructor via appointment to discuss next steps prior to retaking this test. Points: 54/67 1 What is the complementary sequence to 3’ ATG CGA ATA 5’? (1/1 Points) 3’ TAT TCG CAT 5’ The correct answer is 3' TAT TCG CAT 5'. Remember complementary means “Matching or Pairing” You have to remember to pay attention to your numbers as well as your letters (A-T, G-C, 5'-3'). The correct answer is 3’ ATG CGA ATA 5’ (original sequence) 5’ TAC GCT TAT 3’ (complementary sequence) But we asked for it in the 3’ direction, so mirror answer to give correct answer: 3’ TAT TCG CAT 5’ 3’ TAC GCT TAT 5’ 3’ GAT AGC ATA 5’ 5’ ATA AGC GTA 3’ 2 Which amino acid sequence would result from this Coding DNA strand? 3’ ATG CAG ATA 5’? (0/1 Points) Tyr Val Tyr The correct answer is Ile Asp Val. We are starting at the coding strand, and have to remember the relationship between coding DNA and mRNA. These two strands are noncomplementary and parallel. So we copy the coding strand , change T --- U, and then write the mRNA sequence: 3’ ATG CAG ATA 5’ coding 3’ AUG CAG AUA 5’ mRNA Mirror by changing orientation: 5’ AUA GAC GUA 3’ Read chart Ile Asp Val (chart is in direction of 5' --- 3') If you chose Tyr Val Tyr, this answer is incorrect because this is the amino acid sequence that results from the mRNA 5' UAC GUC UAU 3' which would have been complementary to the given coding strand. Coding DNA is non-complementary and parallel to mRNA. Ile Gln Ile Ile Asp Val Leu His Lys 3 Which amino acid sequence would be made from this template DNA strand? 5’ TAT TAC CGA 3’? (1/1 Points) Tyr Tyr Arg Ile Met Ala Ser Val Ile The correct answer is Ser Val Ile because 5’ TAT TAC CGA 3’ template is complementary and antiparallel so 3’ AUA AUG GCU 5’ but it is in the wrong orientation, so mirror 5’ UCG GUA AUA 3’ and read the chart Ser Val Ile Ser His Gln 4 Which of the following are the correct components for a PCR reaction? (1/1 Points) dNTPs, Primer, RNA Polymerase, template RNA dNTPs, Primer, DNA Polymerase, template DNA The correct answer is dNTPs, Primer, DNA Polymerase, template DNA. Notice that all components are about DNA. The Primer is even a DNA Primer. ATP, Primer, mRNA polymerase; template mRNA Acetyl CoA, RNA primer, DNA Ligase, Template phosphate 5 Assuming 100% reaction efficiency, how many DNA copies will be produced after 5 PCR cycles, if we begin with 1 DNA template? (1/1 Points) 16 32 The correct answer is 32. 2x2x2x2x2=32 64 10 6 Which of the following would represent a silent mutation if this is the original sequence: 5’ AUC GUA ACA 3’? (1/1 Points) 5’ AUC GGA ACA 3’ 5' AUA GUA ACA 3' The correct answer is 5' AUC GUA ACA 3' Ile Val Thr