Summary Unit 2C Practical Scientific Procedures and Techniques BTEC Applied
Science
INCLUDES THE CHROMATOGRAPHY ASSIGNMENT AS WELL WITH ALL THE
COMPARISONS AND IMPROVMENTS This assignment biology, chemistry and
physics part of what the teachers ask for in that assignment of BTEC Applied
Science year 3. I was given DISTINCTION without any feedback and its very well
done and explained. All the tasks that the assignment brief asks for I have them
there. I spent more than 10 hours doing it and it has nothing copy and paste from
internet. Feel free and confident to purchase if you dont want to spend your time
doing it and get feedbacks after you submit your assignment.
Unit 2: practical Scientific
Procedures and Techniques.
Part C: undertake chromatographic techniques to identify components in mixtures.
Assignment title: Separate to identify.
Scenario
I’m a newly appointed technical assistant at a large chemical plant, Chemcalequip. As part of my
induction period and to progress in my role, I have to demonstrate skills in a range of practical
procedures and techniques.
I need to demonstrate that I can carry out Chromatographic techniques to separate and identify
components in mixtures.
Paper chromatography introduction
, Paper chromatography is an analytical method used to separate coloured chemicals or
substances. It is primarily used as a teaching tool, having been replaced by other chromatography
methods, such as thin-layer chromatography. A paper chromatography variant, two-dimensional
chromatography involves using two solvents and rotating the paper 90° in between. This is
useful for separating complex mixtures of compounds having similar polarity, for example,
amino acids. The setup has three components.
The mobile phase is a solution that travels up the stationary phase, due to capillary action. The
mobile phase is generally mixture of polar organic solvent with water, while the stationary phase
is water. Paper is used to support stationary phase (water). Difference between TLC and paper
chromatography is that stationary phase in TLC is a layer of adsorbent (usually silica gel, or
aluminium oxide), and stationary phase in paper chromatography is water.
Hypothesis test
The polar components of the analyte will adhere to the silica tightly and thus
travel slowly up the plate, while the less polar or non-polar components will
not adhere that strongly to the silica and travel up the plate relatively fast with the solvent.
Introduction
1/3
Paper chromatography is a process that uses special filter paper to identify the different
substances in a mixture. The substances in the mixture dissolve in the alcohol and move up the
paper. The heavier substances move up the paper more slowly. The lighter substances move up
the paper faster. The enable the heavy and light substances to be separated from one another.
Aim :To find out the colours or pigments presents in spinach leaves by paper chromatography.
Apparatus
Isopropyl alcohol and acetone mixture
Science
INCLUDES THE CHROMATOGRAPHY ASSIGNMENT AS WELL WITH ALL THE
COMPARISONS AND IMPROVMENTS This assignment biology, chemistry and
physics part of what the teachers ask for in that assignment of BTEC Applied
Science year 3. I was given DISTINCTION without any feedback and its very well
done and explained. All the tasks that the assignment brief asks for I have them
there. I spent more than 10 hours doing it and it has nothing copy and paste from
internet. Feel free and confident to purchase if you dont want to spend your time
doing it and get feedbacks after you submit your assignment.
Unit 2: practical Scientific
Procedures and Techniques.
Part C: undertake chromatographic techniques to identify components in mixtures.
Assignment title: Separate to identify.
Scenario
I’m a newly appointed technical assistant at a large chemical plant, Chemcalequip. As part of my
induction period and to progress in my role, I have to demonstrate skills in a range of practical
procedures and techniques.
I need to demonstrate that I can carry out Chromatographic techniques to separate and identify
components in mixtures.
Paper chromatography introduction
, Paper chromatography is an analytical method used to separate coloured chemicals or
substances. It is primarily used as a teaching tool, having been replaced by other chromatography
methods, such as thin-layer chromatography. A paper chromatography variant, two-dimensional
chromatography involves using two solvents and rotating the paper 90° in between. This is
useful for separating complex mixtures of compounds having similar polarity, for example,
amino acids. The setup has three components.
The mobile phase is a solution that travels up the stationary phase, due to capillary action. The
mobile phase is generally mixture of polar organic solvent with water, while the stationary phase
is water. Paper is used to support stationary phase (water). Difference between TLC and paper
chromatography is that stationary phase in TLC is a layer of adsorbent (usually silica gel, or
aluminium oxide), and stationary phase in paper chromatography is water.
Hypothesis test
The polar components of the analyte will adhere to the silica tightly and thus
travel slowly up the plate, while the less polar or non-polar components will
not adhere that strongly to the silica and travel up the plate relatively fast with the solvent.
Introduction
1/3
Paper chromatography is a process that uses special filter paper to identify the different
substances in a mixture. The substances in the mixture dissolve in the alcohol and move up the
paper. The heavier substances move up the paper more slowly. The lighter substances move up
the paper faster. The enable the heavy and light substances to be separated from one another.
Aim :To find out the colours or pigments presents in spinach leaves by paper chromatography.
Apparatus
Isopropyl alcohol and acetone mixture
